Research Line – Applied Oral Biology
Coordinator: Rafael Rodrigues Lima
Starting Date: 03/02/2015
Coordinator: Rafael Rodrigues Lima
Starting Date: 08/01/2013
Amazonian Biodiversity Incorporated into Dental Materials
Coordinator: Roberta Souza D’Almeida Couto
Starting Date: 04/06/2015
Funding: Coordination of Improvement of Higher Level Personnel – (Post-Graduation Aid)
Coordinator: Rafael Rodrigues Lima
Starting Date: 10/03/2014
Funding: Coordination of Improvement of Higher Level Personnel Sup – (Post-Graduation Aid) and Federal University of Pará – Institutional Program of Scientific Initiation Scholarship (PIBIC)
Coordinator: Rafael Rodrigues Lima
Starting Date: 08/01/2013
Funding: Foundation for Support and Research Development – (Financial Aid)
Use of Technological Resources in Tissue Repairing
Coordinator: Patrícia de Almeida Rodrigues da Silva e Souza
Starting Date: 03/16/2015
Funding: Federal University of Pará – Institutional Program of Scientific Initiation Scholarship (PIBIC)
Research Line – Oral Diagnosis
Correlation of Facial Type and Nasopharyngeal Morphology Through Cone Beam Computed Tomography
Coordinator: Fabrício Mesquita Tuji
Starting Date: 08/01/2013
Funding: Federal University of Pará – Institutional Program of Scientific Initiation Scholarship (PIBIC)
Anatomical Study of Canalis Sinuosus on 100 Cone Beam Tomography Exams
Coordinator: Fabrício Mesquita Tuji
Starting Date: 04/03/2012
Coordinator: Sérgio de Melo Alves Junior
Starting Date: 01/01/2012
Coordinator: João de Jesus Viana Pinheiro
Starting Date: 03/11/2014
Research Line – Epidemiology
Indicadores da Saúde Bucal em Indígenas do Vale Médio do Xingu
Coordinator: Antonio David Corrêa Normando
Starting Date: 01/01/2012
Funding: Coordination of Improvement of Higher Level Personnel – (Post-Graduation Aid), Foundation for Research Support of the State of Pará – (Master’s and Doctorate Scholarship Program), Foundation for Research Support of the State of Pará – (Fapespa Official Notice 003/2012 PPSUS – Research Program for SUS), Federal University of Pará – Institutional Program of Scientific Initiation Scholarship (PIBIC) and Federal University of Pará – (Integration Program: Teaching, Research, and Extension)
Epidemiological Survey of Bone Lesions Diagnosed in Belém - PA
Coordinator: Pedro Luiz de Carvalho
Starting Date: 01/01/2010
Funding:
Malocclusions: diagnosis, self-perception and impact on quality of life
Coordinator: Gustavo Antônio Martins Brandão
Starting Date: 01/01/2014
Funding: Federal University of Pará – Institutional Program of Scientific Initiation Scholarship (PIBIC)
The Impact of Malocclusion on the Quality of Life of Amazonian Populations
Coordinator: Antonio David Corrêa Normando
Starting Date: 03/03/2014
Funding:
Epidemiological Profile of Tooth Injuries in Riverside Populations
Coordinator: Juliana Melo da Silva Brandão
Starting Date: 07/03/2016
Funding: Federal University of Pará – Institutional Program of Scientific Initiation Scholarship (PIBIC)
Third molars: changes in the dental arch, impact on the quality of life and prognosis for exodontia
Coordinatores: Antonio David Corrêa Normando
Gustavo Antônio Martins Brandão
Starting Date: 03/02/2015
Funding: Coordination of Improvement of Higher Level Personnel – (Post-Graduation Aid), Foundation for Research Support of the State of Pará – (Master’s and Doctorate Scholarship Program)
Research Line - Clinical and / or Laboratory Study of Dental Materials
Evaluation of Permeability of Radicular Cervical Dentin Facing Different Irrigation Protocols
Coordinator: Oscar Faciola Pessoa
Starting Date: 03/07/2016
Funding: Coordination of Improvement of Higher Level Personnel – (Post-Graduation Aid)
Effect of Excessive Dental Whitening on the Morphology and Mechanical Properties of Dental Enamel
Coordinator: Mário Honorato da Silva e Souza Junior
Starting Date: 01/01/2014
Funding:
Effcts of the Bioactive Agents in the Dental Whitening Treatment
Coordinator: Juliana Melo da Silva Brandão
Starting Date: 03/16/2015
Funding: Coordination of Improvement of Higher Level Personnel – (Post-Graduation Aid)
Influence of irrigation solutions on resistance to root fracture
Coordinator: Patrícia de Almeida Rodrigues da Silva e Souza
Starting Date: 04/06/2015
Funding: Coordination of Improvement of Higher Level Personnel – (Post-Graduation Aid)
Influence of Etidronate on Microhardness and Dentin Roughness
Coordinator: Oscar Faciola Pessoa
Starting Date: 08/03/2015
Funding: Federal University of Pará – Institutional Program of Scientific Initiation Scholarship (PIBIC)
Coordinator: Sandro Cordeiro Loretto
Starting Date: 03/02/2015
Funding: Federal University of Pará – Institutional Program of Scientific Initiation Scholarship (PIBIC)
Research Line – Clinical, Experimental and Applied Therapy
Coordinator: Fabrício Mesquita Tuji
Starting Date: 01/01/2012
Funding: Coordination of Improvement of Higher Level Personnel – (Post-Graduation Aid)
Coordinator: José Thiers Carneiro Junior
Starting Date: 03/16/2015
Funding:
Coordinator: José Thiers Carneiro Junior
Starting Date: 03/02/2015
Funding:
Coordinator: Juliana Melo da Silva Brandão
Starting Date: 08/01/2015
Funding: Federal University of Pará – Institutional Program of Scientific Initiation Scholarship (PIBIC)
Coordinator: Cecy Martins Silva
Starting Date: 08/03/2015
Funding: Federal University of Pará – Institutional Program of Scientific Initiation Scholarship (PIBIC)
Factors Associated with the Duration and Stability of Orthodontic Treatment
Coordinator: Antonio David Corrêa Normando
Starting Date: 02/03/2015
Funding:
Coordinator: Sandro Cordeiro Loretto
Starting Date: 03/07/2016
Funding: Coordination of Improvement of Higher Level Personnel – (Post-Graduation Aid)
Influence of Single File Systems and Retraction Files on Apical Deviation
Coordinator: Juliana Melo da Silva Brandão
Starting Date: 08/01/2015
Funding: Federal University of Pará – Institutional Program of Scientific Initiation Scholarship (PIBIC)
Penetrability of Endodontic Cement after Different Retreatment Protocols: a Confocal Study
Coordinator: Juliana Melo da Silva Brandão
Starting Date: 03/09/2015
Funding: Coordination of Improvement of Higher Level Personnel – (Post-Graduation Aid)
Pulpal Revascularization in Teeth with Incomplete Rhizogenesis: prospective clinical study
Coordinator: Juliana Melo da Silva Brandão
Starting Date: 01/01/2014
Funding: Federal University of Pará – Institutional Program of Scientific Initiation Scholarship (PIBIC)
2015 – Current
Clinical and Radiographic Effects of the Third Molars on the Dental Arch, Periodontium and Quality of Life: parameters to indicate the need for intervention
Description: The third molars are the teeth that are most frequently impacted, with prevalence between 9.5% and 39.5% in different populations. However, 75% of people who receive dental treatment on a regular basis have third molars removed. The decision of removal or preservation of the third molars still raises doubts to the clinician. In part, due to the inaccuracy of the models of prediction of impaction reported in the literature, and also to the scientific works with inconclusive results. Thus, this decision seems to be centered on a preference for each specialty, rather than clinical conduct based on scientific evidence. In the face of so many controversies, the prophylactic removal of the third molars has been adopted under the pretext of preventing future damage associated with various pathologies. Tertiary crowding in adults or the ultimate instability of orthodontic treatment has been associated with the presence of lower third molars, despite the fact that most studies suggest that third molars have an insignificant influence on changes in the lower arch in the long term. The most conservative approach is the careful monitoring of asymptomatic third molars. This conduct is mainly based on the absence of scientific evidence to justify the indication of prophylactic extraction. The objectives of this project are: to examine the ability of specialists to predict the eruption of third molars; to evaluate the impact of the behaviors adopted on patients' quality of life - Risks, benefits and impact of the third molars Exodontics; to examine the changes produced by the third molars in the morphology of the dental arch; Epidemiology of third molars impactation in remote Amazonian populations and the importance of CT in the diagnosis, prognosis and preservation of third molars.
Status: In progress
Nature: Research
Students involved: Undergraduate Courses: (1) / Specialization: (1) / Academic Master’s: (3).
Members: Antonio David Corrêa Normando - Coordinator / Newton Guerreiro da Silva - Member / Brandão, Gustavo Antônio Martins - Member / Armando Lopes Pereira - Member / José Thiers Carneiro Júnior - Member
Number of S, T & A Productions: 3
Number of counselings: 2
2014 – Current
The Impact of Poor Occlusion and Dental Loss on the Quality of Life of Amazonian Populations
Description: The epidemiological knowledge makes it possible to evaluate the interference of etiological factors in the occurrence of the disease, providing data for the planning of preventive and curative actions. Assessing the effect of oral conditions on quality of life provides important information for researchers, for the patient and for the health system, in addition to estimating the need for treatment. Because it is a public health problem, it is necessary to understand the biopsychosocial aspects of malocclusion and its effect on individuals' quality of life. The study of the quality of life in orthodontic patients is of fundamental importance in understanding the impact of malocclusions in their daily life, especially in relation to functional limitations and psychosocial well-being, besides making possible the planning of strategies of action and destination resources where their employment will be most effective. There is currently no data to evaluate the relationship between malocclusion, dental losses and quality of life in indigenous, riverside and urban communities in the Amazon region. Universal Call - MCTI / CNPq Nº 14/2013. R$ 54,000.00. CAPES Master’s Scholarship. PIBIC-CNPq Scholarship.
Status: In progress
Nature: Research
Students involved: Undergraduate Courses: (1) / Academic Master’s: (2)
Members: Antonio David Corrêa Normando - Coodinator / Newton Guerreiro da Silva - Member / Gustavo Antônio Martins Brandão - Member.
Funding: National Council for Scientific and Technological Development - Financial Aid
Number of S, T & A Productions: 2
Number of counselings: 2
2013 – Current
Indicadores da Saúde Bucal em Indígenas do Vale Médio do Xingu
Description: The scientific literature presents a few studies on the epidemiological characteristics of oral health conditions and biometric data on the morphofunctional characteristics of the Brazilian natives' dentition and face. In addition, there are no reports on the effects of miscegenation, resulting from the fission and fusion processes of indigenous groups, and loss of identity through contact with non-Indians in oral health. Regarding the indigenous populations of the middle valley of Xingu, there is also a recent increase in migratory flows due to the construction of the Belo Monte hydroelectric plant in the region known as the great Xingu. Such studies are important to define important epidemiological aspects related to the characterization of these populations, as well as the understanding of how the anthropomorphic Indian traits contributed to the formation of the Brazilian population, notoriously mixed. In this perspective, the universal contribution that the study of relatively isolated populations can give to the understanding of the influence of the habits and customs of modern man in the etiology of oral diseases. Thus, the objective of this research is to investigate oral health indicators and possible associated etiological factors by conducting a wide survey of the occurrence of oral diseases in the indigenous populations of the middle valley of the Xingu River and its Iriri and Bacajá affluents. Biometric studies on the dentofacial characteristics of these indigenous groups will also be carried out. The sample of the present study will be collected from the examination of the entire indigenous population allocated in the Altamira Indigenous Special Sanitary District (DSEI). This district, with its head office in the municipality of Altamira – state of Pará-Brazil, is composed of 2,141 indigenous people, from 16 villages, belonging to 3 linguistic groups and 9 ethnicities. This scientific research will contribute greatly to the implementation of basic health care programs, based on the family health strategy, taking into account the specific needs of each population group. Also, the benefits of acquiring scientific knowledge about the anthropomorphic characteristics of the Amazonian indigenous populations are highlighted.
Status: In progress
Nature: Research
Students involved: Undergraduate Courses: (2) / Academic Master’s: (2)
Members: Antonio David Corrêa Normando - Coordinator / João de Jesus Viana Pinheiro - Member
Funding: Paraense Amazon Foundation for Research Support - Financial Aid
Number of S, T & A Productions: 5
Number of counselings: 2
2012 – 2016
Avaliação do Efeito do Envelhecimento Intraoral dos Materiais Ortodônticos
Description:
This project aims to evaluate changes in orthodontic materials, brackets, wires and ligatures, orthodontics before and after exposure to the oral environment. The impact of the color change of the elastomers on the aesthetics of the materials, the accumulation of debris and their effect on the friction during the sliding mechanics between the wire and the bracket will be investigated, comparing brackets with conventional ligature system and brackets. Also, the effect of cleaning the materials on the mechanical properties of the material. Funding through PIBIC Scholarship and PARD-UFPA Official Notice.
Status: Completed
Nature: Research
Students involved: Undergraduate Courses: (4) / Specialization: (1) / Academic Master’s: (2) / Professional Master’s: (0) / Doctorate: (0)
Members: Antonio David Corrêa Normando - Coordinator / Isabella Silva Vieira Marques - Member / Adriana M Araújo - Member / Raíssa Costa Araújo - Member / Livia Bichara - Member / Brisa dos Santos Leite - Member / Mônica L. C. Aragón - Member / Leandro Santiago Lima – Member
Number of S, T & A Productions: 7
2011 – 2012
Prevalence of Dental Malocclusion in Indigenous Populations of the Middle Xingu
Description: Pro-doctoral
Status: In progress;
Nature: Research
Students involved: Undergraduate Courses: (1)
Members: Antonio David Corrêa Normando - Coordinator / Elma Pinto Vieira – Member
Funding: Federal University of Pará - Financial Aid
2009 – Current
Colective Oral Health in the Amazon – SBCA (Portuguese acronym)
Description: The group proposes, through proposals of collective health, to carry out epidemiological studies on the oral health of the Amazon, mainly to the State of Pará, with priority for neglected populations. With the knowledge of the epidemiological profile, the group proposes to collaborate in the elaboration of public policies of Dentistry for the State, through the analysis of the management and evaluation of the programs and services within the SUS. It is also proposed to research materials and dental techniques that contemplate the substances that compose Amazonian biodiversity.
Status: In progress
Nature: Research.
Members: Antonio David Corrêa Normando - Coordinator
1993 – 1994
Basic and Limnological Studies of the Guamá River
Description: 1983/84 – Participation through sampling. Responsible for the project: Professor Alexandre Guzman Achá, UFPA.
Status: Completed
Nature: Research
Students involved: Undergraduate Courses: (0) / Specialization: (0) / Academic Master’s: (0) / Professional Master’s: (0) / Doctorate: (0)
Members: Antonio David Corrêa Normando - Coordinator
1988 – 1991
Evaluation of the Craniofacial Pattern in Leukoderma Normal Occlusion Carriers Resident in Baurú, São Paulo
Description:
Participation in scientific research through sample collection and team coordination. 1988 - Participation through sampling. 1990 / 91 – Participation through the organization and coordination of the team responsible for the sample collection. Responsible for the project: Professor Leopoldino Capelloza Filho, HPRLLP-USP.
Status: Completed
Nature: Research
Members: Antonio David Corrêa Normando - Coordinator
2125200 - Armando Rodrigues Lopes Pereira Neto (743.168.772-91)
2017 – Current
In Vitro Study of the Behavior of Human Gingival Fibroblasts and Osteoblasts on Discs of Different Materials: Titanium and Stainless Steel
Description: Dental implants and their prosthetic components usually consist of alloys derived from commercially pure titanium (TiCP – Portuguese acronym) and this material presents excellent compatibility when in contact with biological structures, stimulating the induction of chemical mediators of the organism that will help healing and directly influence their longevity. These artifacts need constant studies and special understanding because the oral cavity is present both in the internal environment, bone and in the external environment, and is therefore directly susceptible to the harmfulness of bacteria and other microorganisms and harmful substances of the bacterial plaque that are prevented to penetrate into the gingival sulcus because of the important biological sealing relationship that exists between the adhesion of the peri-implant gingiva and the prosthetic abutment. Given that the surface roughness is directly related to the accumulation of dental plaque and consequent tissue inflammation, new materials have been proposed for the preparation of these components. The use of Stainless Steel is reported in the literature with high success rates with respect to its mechanical properties, biocompatibility and cost effectiveness. ASTM F138 stainless steel is already used for the manufacture of some of these prosthetic abutments. Thus, the objective of this work is to verify in vitro the behavior of human gingival fibroblasts and human osteoblasts in experimental discs composed of different materials: titanium and stainless steel.
Status: In progress
Nature: Research
Students involved: Academic Master’s: (2)
Members: Armando Rodrigues Lopes Pereira Neto - Coordinator / Felipe Rezende de Albuquerque - Member / Giovanni Pereira Mitre - Member / Maria Sueli da Silva Kataoka - Member / Sergio de Melo Alves Junior - Member
2015 – Current
Clinical and Radiographic Effects of the Third Molars on the Dental Arch, Periodontium and Quality of Life: parameters to indicate the need for intervention
Description: The third molars are the teeth that are most frequently impacted, with prevalence between 9.5% and 39.5% in different populations. However, 75% of people who receive dental treatment on a regular basis have third molars removed. The decision of removal or preservation of the third molars still raises doubts to the clinician. In part, due to the inaccuracy of the models of prediction of impaction reported in the literature, and also to the scientific works with inconclusive results. Thus, this decision seems to be centered on a preference for each specialty, rather than clinical conduct based on scientific evidence. In the face of so many controversies, the prophylactic removal of the third molars has been adopted under the pretext of preventing future damage associated with various pathologies. Tertiary crowding in adults or the ultimate instability of orthodontic treatment has been associated with the presence of lower third molars, despite the fact that most studies suggest that third molars have an insignificant influence on changes in the lower arch in the long term. The most conservative approach is the careful monitoring of asymptomatic third molars. This conduct is mainly based on the absence of scientific evidence to justify the indication of prophylactic extraction. The objectives of this project are: to examine the ability of specialists to predict the eruption of third molars; to evaluate the impact of the behaviors adopted on patients' quality of life - Risks, benefits and impact of the third molars Exodontics; to examine the changes produced by the third molars in the morphology of the dental arch; Epidemiology of third molars impactation in remote Amazonian populations and the importance of CT in the diagnosis, prognosis and preservation of third molars.
Status: In progress
Nature: Research
Students involved: Academic Master’s: (2)
Members: Armando Rodrigues Lopes Pereira Neto - Coordinator / Gustavo Antônio Martins Brandão - Member / Antonio David Correa Normando - Member / José Thieres Carneiro Junior - Member / Newton Guerreiro da Silva Junior – Member
2010 - 2013
Comparative Analysis of diferente types of Peri-Implantitites Treatments
Project certified by Coordinator Marco Aurélio Bianchini on 07/14/2014.
Description: This project aims to evaluate the periodontal index in surgical and non-surgical peri-implantite treatments, as well as the condition of the peri-implant tissues after implant installation.
Status: Completed
Nature: Research
Students involved: Academic Master’s: (2); Doctorate: (3).
Members: Armando Rodrigues Lopes Pereira Neto - Member / Ernesto Barquero Cordero - Member / João Gustavo Oliveira de Souza - Member / Marco Aurélio Bianchini - Coordinator / José Moisés de Souza Júnior - Member / Guenther Schuldt Filho – Member
Number of S, T & A Productions: 2
2010 – 2012
Análise do Grau de Calibração de Diferentes Tipos de Torquímetros Protéticos: estudo in vitro
Description: The objective of this study is to analyze the degree of calibration of prosthetic torque wrenches used in private practice in Florianópolis.
Status: Completed
Nature: Research
Students involved: Undergraduate Courses: (1) / Academic Master’s: (1) / Doctorate: (1)
Members: Armando Rodrigues Lopes Pereira Neto - Coordinator / Antonio Carlos Cardoso – Member / Gustavo Castellazzi Sella – Member / Diego Klee de Vasconcellos – Member / Claudia Sens – Member
2008 - 2010
Culture of Fibroblasts and Osteoblasts on a PLDLA Framework
Description: To evaluate the adhesion and proliferation of osteoblastic and fibroblast cells in three-dimensional cell culture performed in a PLDLA framework as a potential framework for tissue engineering.
Status: Completed
Nature: Research
Students involved: Academic Master’s: (1)
Members: Armando Rodrigues Lopes Pereira Neto - Coordinator / César Augusto Magalhães Benfatti - Member / Ricardo de Souza Magini - Member
2008 - 2009
Histomorphometric evaluation of Algenic Bone Tissue and Demineralized Organic / Inorganic Bone (GenMix®) in Elevation of Maxillary Sinus Floor and its Influence on the Success of Osseointegrated Implants
Status: Completed
Nature: Research
Students involved: Academic Master’s: (6) / Professional Master’s: (1)
Members: Armando Rodrigues Lopes Pereira Neto - Coordinator / César Augusto Magalhães Benfatti - Member / Ricardo de Souza Magini - Member / Ernesto Barquero Cordero - Member / Gustavo Castellazi Sella - Member / João Gustavo Oliveira de Souza - Member / Pamela Candida Aires Ribas de Andrade - Member / Newton Bergamashi Lucchiari Junior - Member / Daniel Kfouri Lopes - Member.
327584 - Cecy Martín Silva (157925792-53)
2017 - Current
Effect of Casein Phosphopeptide – Fluorinated Amorphous Calcium Phosphate (CPP-ACPF) and Low-Power Laser on Decreased Dentin Hypersensitivity: a randomized, double-blind, placebo-controlled clinical study
Status: In progress
Nature: Research
Students involved: Undergraduate Courses: (1) / Academic Master’s: (3).
Members: Cecy Martins Silva - Coordinator / Jesuína Lamartine Nogueira Araújo - Member / Renata Antunes Esteves - Member / Eliane Bemerguy Alves - Member / Beatriz Helena Rodrigues e Silva - Member / Cristiane de Melo Alencar - Member / Brennda Lucy Freitas de Paula - Member / Mariangela Ivette Guanipa Ortiz - Member.
Funding: Coordination of Improvement of Higher Level Personnel – Scholarship / Institutional Program of Scientific Initiation Scholarship – PIBIC – UFPA – Scholarship / National Council for Scientific and Technological Development – Scholarship.
Number of counselings: 1
2017 - Current
Effect of Nano-Hydroxyapatite and Low-Power LASER on Dentin Hypersensitivity Control: a randomized, double-blind, placebo-controlled clinical study
Description: The aim of this study is to evaluate the effect of nano-hydroxyapatite associated with and without and low-power laser therapy (TLBI) in eliminating or reducing dentin hypersensitivity in a randomized, placebo-controlled clinical trial.
Status: In progress
Nature: Research
Students involved: Undergraduate Courses: (2) / Academic Master’s: (3)
Members: Cecy Martins Silva - Coordinator / Jesuína Lamartine Nogueira Araújo - Member / Renata Antunes Esteves - Member / Eliane Bemerguy Alves - Member / Cristiane de Melo Alencar - Member / Brennda Lucy Freitas de Paula - Member / Mariangela Ivette Guanipa Ortiz - Member / Rayssa Costa da Silva Ribeiro - Member / Danielle da Silva Pompeu - Member.
Funding: Federal University of Pará - Scholarship / National Council for Scientific and Technological Development – Scholarship.
Number of counselings: 1
2017 - Current
Analysis of the Influence of Desensitizing Treatments on the Pulmonary Inflammatory Response of Bleached Teeth
Description: Project approved under ordinance No. 244/2017-ICS, aims to evaluate the inflammatory pulp response of healthy human premolars after bleaching treatment with hydrogen peroxide at 35%, and to compare the efficacy of six desensitizing agents in the mechanism of pulpal repair. Ordinance No. 244/2017-ICS.
Status: In progress
Nature: Research
Students involved: Undergraduate Courses: (4) / Academic Master’s: (2)
Members: Cecy Martins Silva - Coordinator / Yasmin do Socorro Batista de Lima Gomes - Member / Beatriz Helena Rodrigues e Silva - Member / Cristiane de Melo Alencar - Member / Brennda Lucy Freitas de Paula - Member / Cláudia Pires Rothbart - Member / Roberta Souza D'Almeida Couto - Member / Rafael Rodrigues Lima - Member / Rayssa Costa da Silva Ribeiro - Member / Danielle da Silva Pompeu - Member.
Funding: National Council for Scientific and Technological Development - Scholarship / Federal University of Pará - Scholarship.
Number of counselings: 3
2016 - 2017
Effect of 1.1% Sodium Fluoride Associated with or not on the Low Intensity Laser and on the Prevention of Post-Bleaching Sensitivity
Description: The aim of this clinical study was to evaluate the effect of sodium fluoride on 1.1% associated with low power laser after dental bleaching, in the prevention of postoperative sensitivity during 2 months of follow-up. Ordinance No. 027/2017-ICS.
Status: Completed
Nature: Research
Students involved: Undergraduate Courses: (1) / Academic Master’s: (1)
Members: Cecy Martins Silva - Coordinator / Jesuína Lamartine Nogueira Araújo - Member / Renata Antunes Esteves - Member / Eliane Bemerguy Alves - Member / Cristiane de Melo Alencar - Member / Brennda Lucy Freitas de Paula - Member / Roberta Souza D'Almeida Couto - Member.
Funding: Institutional Program of Scientific Initiation Scholarship - PIBIC - UFPA - Scholarship / PROPESP/FADESP Support Program for Qualified Publishing - PAPQ- UFPA - Other.
Number of S, T & A Productions: 2
Number of counselings: 1
2016 - 2017
In Vitro Effect of Arginine at Different Concentrations Associated with Calcium Carbonate after Dental Whitening
Description: In vitro study, the effect of dentifrices containing different concentrations of arginine (8% and 12%) associated with 40% calcium carbonate, applied on the enamel whitened with hydrogen peroxide in high concentration in the hardness, roughness, morphology and color.
Status: Completed
Nature: Research
Students involved: Undergraduate Courses: (3)
Members: Cecy Martins Silva - Coordinator / Jesuína Lamartine Nogueira Araújo - Member / Eliane Bemerguy Alves - Member / Beatriz Helena Rodrigues e Silva - Member / Cristiane de Melo Alencar - Member / Brennda Lucy Freitas de Paula - Member / Ana Daniela Silva da Silveira - Member / Fabiana Brito da Cunha - Member.
Funding: Institutional Program of Scientific Initiation Scholarship- PIBIC - UFPA - Scholarship.
Number of S, T & A Productions: 1
2016 - 2017
Does Fluoride in High Concentrations Prevent Changes in Bleached Enamel?
Description: In vitro evaluation of the effect of fluoride in high concentration (5000 pmm) on the surface of human dental enamel after bleaching with 35% hydrogen peroxide by Vickers microhardness, roughness and colorimetry.
Status: Completed
Nature: Research
Students involved: Undergraduate Courses: (3)
Members: Cecy Martins Silva - Coordinator / Jesuína Lamartine Nogueira Araújo - Member / Eliane Bemerguy Alves - Member / Beatriz Helena Rodrigues e Silva - Member / Cristiane de Melo Alencar - Member / Brennda Lucy Freitas de Paula - Member / Ana Daniela Silva da Silveira - Member / Fabiana Brito da Cunha - Member / Brenna Magdalena Lima Nogueira - Member.
Funding: PROPESP/FADESP Support Program for Qualified Publishing - PAPQ- UFPA - Other / Institutional Program of Scientific Initiation Scholarship - PIBIC - UFPA - Scholarship.
2016 - 2016
Rheological Properties of Low, Medium and High Viscosity Composite Resins
Description: Design approved under Ordinance No. 024/2016? ICS. It aims to evaluate the rheological properties of composite resins of low, medium and high viscosity.
Status: Completed
Nature: Research
Students involved: Undergraduate Courses: (2) / Academic Master’s: (1).
Members: Cecy Martins Silva - Member / Eliane Berneguy Alves - Member / Jesuína Lamartine Nogueira Araújo - Coordinator.
2016 - Current
Effect of Low Potential Laser Associated with Potassium Nitrate on Post-Bleaching Sensitivity Control: a randomized, controlled, double-blind, split-mouth clinical trial
Description: Clinically evaluate the effect of Low Potential Laser Therapy (TLBP – Portuguese acronym) associated with potassium nitrate in the control of postsurgical pain sensitivity caused by office dental whitening. The null hypothesis tested in the present study will be: H0 - there will be no difference in postsurgical sensitivity between the bleached groups due to the association of two treatments with desensitizing action (TLBP / KNO3) when compared to the use of KNO3 or only to the use of TLBP in the different evaluation periods.
Status: In progress
Nature: Research
Students involved: Undergraduate Courses: (1) / Academic Master’s: (2)
Members: Cecy Martins Silva - Coordinator / Jesuína Lamartine Nogueira Araújo - Member / Eliane Bemerguy Alves - Member / Beatriz Helena Rodrigues e Silva - Member / Cristiane de Melo Alencar - Member / Brennda Lucy Freitas de Paula - Member / Roberta Souza D'Almeida Couto - Member.
Funding: National Council for Scientific and Technological Development - Scholarship / Coordination of Improvement of Higher Level Personnel - Scholarship / Institutional Program of Scientific Initiation Scholarship- PIBIC - UFPA – Scholarship.
Number of S, T & A Productions: 1
Number of counselings: 1
2015 - 2017
Clinical, Randomized Study of Postsurgical Sensitivity after Nanohydroxyapatite and Arginine-Associated Bleaching Treatment
Description: To assess the efficacy of nanohydroxyapatite and arginine in preventing sensitivity and color changes after home bleaching treatment for 3 months of follow-up. 57 patients will be randomized by numerical draw and allocated in 3 groups (n = 20): G1, treated with only 22% carbamide peroxide (PC 22%); G2, treated with 22% PC followed by application of arginine; G3, treated with PC 22% followed by the application of nano-hydroxyapatite. To evaluate the postsurgical sensitivity, each volunteer will receive a questionnaire containing a modified visual scale (VAS) in which they will report the perceived sensitivity after clinical evaluation by means of a triple syringe. For color evaluation, a spectrophotometer will be used that will gauge the color of the upper incisors. Color and sensitivity assessments were performed before and after 1, 30, 60 and 90 days of bleaching treatment. The results will be analyzed by the Friedman test (p> 0.05).
Status: Completed
Nature: Research
Students involved: Undergraduate Courses: (2)
Members: Cecy Martins Silva - Coordinator / Jesuína Lamartine Nogueira Araújo - Member / Cristiane de Melo Alencar - Member / Ranna Castro da Silva - Member / Ana Daniela Silva da Silveira - Member.
Funding: Institutional Program of Scientific Initiation Scholarship - PIBIC - UFPA - Scholarship / PROPESP/FADESP Support Program for Qualified Publishing - PAPQ- UFPA - Other.
Number of S, T & A Productions: 1
2015 - 2017
In vitro Study of the Effect of Strontium Chloride and Potassium Nitrate Associated with Home Bleaching Treatment on Bovine Enamel
Description: In vitro study of the effect of 10% strontium chloride and 2% potassium nitrate of bovine enamel whitened with 35% hydrogen peroxide by means of the Knoop microhardness, surface roughness and colorimetry triistimulus.
Status: Completed
Nature: Research
Students involved: Undergraduate Courses: (2)
Members: Cecy Martins Silva - Coordinator / Jesuína Lamartine Nogueira Araújo - Member / Renata Antunes Esteves - Member / Eliane Bemerguy Alves - Member / Cristiane de Melo Alencar - Member / Ana Daniela Silva da Silveira - Member / Brennda Lucy Freitas de Paula - Member.
Funding: Institutional Program of Scientific Initiation Scholarship (PIBIC- UFPA- AF) – Scholarship
2015 - 2017
Evaluation of the Efficacy of Low Intensity Laser in the Treatment of Post-Bleaching Dental Hypersensitivity
Description: Clinical controlled study of the effectiveness of the low-intensity laser associated to dental bleaching, in the prevention of postsurgical sensitivity and alteration of tooth color.
Status: Completed
Nature: Research
Students involved: Undergraduate Courses: (2)
Members: Cecy Martins Silva - Coordinator / Jesuína Lamartine Nogueira Araújo - Member / Eliane Bemerguy Alves - Member / Cristiane de Melo Alencar - Member / Ana Daniela Silva da Silveira - Member / Caroline Caldeira e Silva - Member / Brenna Magdalena Lima Nogueira – Member
Funding: Institutional Program of Scientific Initiation Scholarship – PIBIC – UFPA – Scholarship
2015 - 2016
Clinical Study of the Effect of Potassium Nitrate and Chloride of Strontium Associated with Home Bleaching Treatment
Description: Clinical controlled study of the potassium nitrate and strontium chloride effectiveness associated with home bleaching treatment in the prevention of postsurgical sensitivity and enamel color change during 3 months of follow-up.
Status: Completed
Nature: Research
Students involved: Undergraduate Courses: (2)
Members: Cecy Martins Silva - Coordinator / Jesuína Lamartine Nogueira Araújo - Member / Renata Antunes Esteves - Member / Eliane Bemerguy Alves - Member / Cristiane de Melo Alencar - Member / Ana Daniela Silva da Silveira - Member / Ranna Castro da Silva - Member
Funding: Institutional Program of Scientific Initiation Scholarship- PIBIC - UFPA - Scholarship / PROPESP/FADESP Support Program for Qualified Publishing - PAPQ- UFPA – Other
Number of S, T & A Productions: 3
Number of counselings: 1
2015 - 2015
Comparative Analysis of Third Generation LEDs Used in the Photoactivation of Nanoparticulate Composite Resins, Through Nanohardness, Modulus of Elasticity and Degree of Monomeric Conversion
Project certified by Coordinator Jesuina Lamartine Nogueira Araújo on 12/01/2017.
Description: Ordinance No. 014/2015 – ICS. This study aims at the comparative evaluation of third generation LEDs used in the photoactivation of nanoparticulate composite resins through nanodurity, modulus of elasticity and degree of monomeric conversion.
Status: Completed
Nature: Research
Students involved: Undergraduate Courses: (2)
Members: Cecy Martins Silva - Member / Jesuína Lamartine Nogueira Araújo - Coordinator / Eliane Bemerguy Alves – Member
2015 - Current
Efeitos de Agentes Bioativos no Tratamento do Clareamento Dental
Description: O projeto visa analisar os efeitos de agentes bioativos sobre a superfície do esmalte dental diante do clareamento dental. As análises in vitro observarão a morfologia macro e microscópica do tecido dental antes e após o clareamento. O projeto integra diversas especialidades em quatro planos de trabalho inicialmente propostos: 1) O flúor em elevadas concentrações previne alterações no esmalte clareado - avaliação in vitro o efeito do flúor em alta concentração (5000 pmm) sobre a superfície de esmalte dental humano após clareamento com peróxido de hidrogênio a 35% por meio da microdureza Vickers, rugosidade e colorimetria. 2) O efeito do cloreto de estrôncio e do nitrato de potássio associados ao tratamento clareador caseiro no esmalte bovino. - estudo in vitro do efeito do cloreto de estrôncio a 10% e do nitrato de potássio a 2% do esmalte bovino clareado com peróxido de hidrogênio a 35% por meio da microdureza Knoop, rugosidade superficial e colorimetria triistimulus. 4) Efeito in vitro da arginina em diferentes concentrações associado ao carbonato de cálcio após clareamento dental: estudo in vitro do efeito de dentifrícios contendo diferentes concentrações de arginina (8% e 12%) associadas ao carbonato de cálcio 40%, aplicados sobre o esmalte clareado com peróxido de hidrogênio em alta concentração na dureza, rugosidade, morfologia e cor do esmalte.
Status: In progress
Nature: Research
Students involved: Undergraduate Courses: (2) / Academic Master’s: (1)
Members: Cecy Martins Silva - Coordinator / Oscar Faciola Pessoa - Member / Mário Honorato Silva e Souza Júnior - Member / Sandr C. Loreto - Member / Roberta Souza D'Almeida Couto - Member / Juliana Melo da Silva - Member / Patricia de Almeida Rodrigues da Silva e Souza – Member
Funding: Institutional Program of Scientific Initiation Scholarship - PIBIC - UFPA - Scholarship / Coordination of Improvement of Higher Level Personnel - Scholarship
Number of S, T & A Productions: 6
2015 - Current
Is the Bubaline Substrate a Human-Suitable Substitute in the Bonding Resistance Tests of Dental Materials?
Description: Since the adhesion of restorative materials to the tooth structure was achieved, where the first step was the enamel acid conditioning introduced by Buonocore (1955), this factor has represented a great achievement in the development of restorative dentistry, allowing for more conservative clinical treatments. Garcia (2007) describes that such technique makes possible the accomplishment of aesthetic, conservative and preventive restorative procedures. Since then, several generations of adhesive systems have been presented and all with the same purpose: to provide greater bond strength between the adhesive material and the dental remnant. Therefore, the need for evaluation and comparison of the adhesive capacity of the materials offered for clinical applicability is clear, given the wide range of these materials (Kadam et al., 2013; Grassi et al., 2012). According to Yanssen et al. (2013), the difficulty of obtaining sample size with quality and the impossibility of standardization (control of the source and age of the human teeth), the risks of infection and the ethical aspects involved, has made the teeth of mammalian animals used as alternatives to human teeth in in vitro and in situ studies. What had previously been recommended (Rueggeberg et al., 1991; Mellberg et al., 1992; Zero et al., 1995; Skene et al., 2002; Camargo et al., 2007). As substitutes, bovine teeth are widely used in the most diverse specialties, due to their micro and macroscopic similarity to human teeth, ease of acquisition, and low caries index. According to Schilke et al. (2000), the permanent inferior bovine incisors are frequently used due to the similarities of size and curvature with superior central incisors. The teeth of other mammalian animals (sheep, horses and pigs) have also been used as substitutes for human teeth in dental research. Nogueira et al., (2014) compared the ultrastructure and physical properties of bovine, bubaline and human enamel, concluding that the morphology, microhardness and mineral composition of the dental enamel of the animals analyzed were similar to human dental tissue. In this way, it made the bubaline substrate - enamel level - a reference for later studies. In this sense, the literature is still limited comparing the buffalo dental substrates to the human, and totally non-existent as to its use as another alternative for bond strength tests. Other clarifications must also be made regarding the morphological characteristics of bubaline dentin and to evaluate if there is similarity of this one with human dentin. According to Barbosa (2005) and Santana et al. (2011), it is mainly in the Northern region of Brazil where the buffalo herd can be found (about 50%). Thus, it is evident that, by offering the bubaline substrate as another viable and efficient alternative to the human substrate in mechanical tests, this will entail more subsidies for the accomplishment of several tests within the research in Dentistry. This research will focus on understanding the evolution of dental materials and can be performed to evaluate the bond strength of these dental substrates.
Status: In progress
Nature: Research
Students involved: Undergraduate Courses: (1) / Academic Master’s: (1)
Members: Cecy Martins Silva - Member / Mário Honorato Silva e Souza Júnior - Member / Sandr C. Loreto – Coordinator
2014 - 2015
In Situ and In Vivo Study of the Effects of Bioactive Agents on Bleached Enamel
Description: Approved by Ordinance No. 0311/2014 - ICS, the objective of this work will be to evaluate, in situ and in vivo, the effects of different bioactive agents (NovaMin®, CPP-ACP and nanohydroxyapatite) applied to human enamel after bleaching with 37.5%, by means of the following evaluation methods: Knoop microhardness, surface roughness, ion chromatography, scanning electron microscopy, measurement of pain sensitivity and spectrophotometry.
Status: Completed
Nature: Research
Students involved: Undergraduate Courses: (6)
Members: Cecy Martins Silva - Coordinator / Jesuína Lamartine Nogueira Araújo - Member / Renata Antunes Esteves - Member / Eliane Bemerguy Alves - Member / Hilton Túlio Costi - Member / Kelson Fayal – Member
Funding: Institutional Program of Scientific Initiation Scholarship- PIBIC - UFPA - Scholarship / PROPESP/FADESP Support Program for Qualified Publishing - PAPQ- UFPA - Other / Paraense Amazon Foundation for Research Support - Scholarship
Number of counselings: 4
2014 - 2014
Study of the Ethanol Influence on Microhardness and Toughness of Resin Cement Fractions
Status: Completed
Nature: Research
Students involved: Undergraduate Courses: (1)
Members: Cecy Martins Silva - Member / Renata Antunes Esteves - Coordinator / Simone Soares Pedrosa – Member
Funding: Institutional Program of Scientific Initiation Scholarship – PIBIC - UFPA - Scholarship
2013 - 2015
Randomized Clinical Study of the Effect of Sodium Phosphosilicate and the Amorphous Calcium Phosphate Casein Phosphopeptide with Fluoride Associated with Dental Whitening
Status: Completed
Nature: Research
Students involved: Undergraduate Courses: (2)
Members: Cecy Martins Silva - Coordinator / Renata Antunes Esteves - Member / Larissa Dias Alexandrino - Member / Cristiane de Melo Alencar - Member / Ana Daniela Silva da Silveira – Member
Funding: Institutional Program of Scientific Initiation Scholarship- PIBIC - UFPA - Scholarship / PROPESP/FADESP Support Program for Qualified Publishing - PAPQ- UFPA - Outra / National Council for Scientific and Technological Development – Scholarship
Number of S, T & A Productions: 1
Number of counselings: 1
2012 - 2014
Effect of Hydrogen Peroxide at 35% with Different Fluorine Concentrations and pH on the Hardness, Roughness and Morphology of Enamel
Status: Completed
Nature: Research
Students involved: Undergraduate Courses: (2)
Members: Cecy Martins Silva - Coordinator / Jesuína Lamartine Nogueira Araújo - Member / Renata Antunes Esteves - Member / Eliane Bemerguy Alves - Member / Hilton Túlio Costi - Member / José Otávio Carrera Silva Júnior – Member
Funding: Institutional Program of Scientific Initiation Scholarship- PIBIC - UFPA – Scholarship
Number of S, T & A Productions: 1
Number of counselings: 1
2012 - 2014
In Situ Study of Bleached Enamel with Bleaching Agents with Fluoride
Status: Completed
Nature: Research
Students involved: Undergraduate Courses: (3)
Members: Cecy Martins Silva - Coordinator / Renata Antunes Esteves - Member / Hilton Túlio Costi - Member / Kelson Fayal - Member / Kleber Fayal - Member / Thais de Mendonça Petta - Member.
Funding: National Council for Scientific and Technological Development - Scholarship / PROPESP/FADESP Support Program for Qualified Publishing - PAPQ- UFPA – Other
Number of counselings: 1
2012 - 2013
The In Vitro and In Situ Effects of Hydroxyapatite on the Enamel Submitted to the Bleaching Treatment
Description: Ordinance: 119/2012-ICS
Status: Completed
Nature: Research
Students involved: Undergraduate Courses: (5) / Doctorate: (2)
Members: Cecy Martins Silva - Coordinator / Eliane Berneguy Alves - Member / Jesuína Lamartine Nogueira Araújo - Member / Renata Antunes Esteves - Member / Danielle Tupinambá Emmi - Member / Hilton Túlio Costi - Member / Hervé Louis Ghislain Rogez – Member
Funding: Paraense Amazon Foundation for Research Support - Scholarship / PROPESP/FADESP Support Program for Qualified Publishing - PAPQ- UFPA - Other / Institutional Program of Scientific Initiation Scholarship- PIBIC - UFPA - Scholarship
Number of counselings: 2
2011 - 2013
Evaluation of Calcium Hydrogen Peroxide in Bovine Enamel Whitening
Status: Completed
Nature: Research
Students involved: Undergraduate Courses: (3)
Members: Cecy Martins Silva - Coordinator / Eliane Bemerguy Alves - Member / Hilton Túlio Costi - Member / Hervé Louis Ghislain Rogez - Member / Kelson Fayal - Member / Kleber Fayal – Member
Funding: National Council for Scientific and Technological Development - Scholarship / Paraense Amazon Foundation for Research Support - Scholarship
Number of S, T & A Productions: 3
Number of counselings: 1
2010 - 2012
Influence of Dental Whitening on the Surface Roughness of Low Contraction Composite Resin
Description: Approved by UFPA’s Health Science Institute. Ordinance No. 022/2010 - GAB/DIR/ICS.
Status: In progress
Nature: Research.
Students involved: Undergraduate Courses: (1)
Members: Cecy Martins Silva - Member / Mário Honorato Silva e Souza Júnior - Member / Sandro Cordeiro Loretto – Coordinator
2010 - 2011
Evaluation of the Effects of Disinfection of Thermopolymerizable Acrylic Resin with Different Sanitizing Solutions
Description: Ordinance No. 109/2011-GD/ICS
Status: Completed
Nature: Research
Students involved: Undergraduate Courses: (1)
Members: Cecy Martins Silva - Member / Renata Antunes Esteves – Coordinator
Funding: Federal University of Pará – Other
Number of S, T & A Productions: 1
Number of counselings: 1
2008 - 2011
In Vitro Evaluation of Properties and Microstructure of Bovine Dental Enamel Subjected to Bleaching by Surface Roughness, Tristimulus Colorimetry, Bracket Bonding Shear Strength, Vickers Microhardness and SEM
Description: Research Project approved by FAPESPA/PA according to the rules of the SECTAM/CNPq Third Official Notice.
Status: In progress
Nature: Research
Students involved: Undergraduate Courses: (10) / Specialization: (2)
Members: Cecy Martins Silva - Coordinator / Jesuína Lamartine Nogueira Araújo - Member / Eliza Burlamaqui Klautau - Member / Oscar Faciola Pessoa - Member / Eliane Bemerguy Alves - Member / Kátia Regina Hostílio Cervante Dias - Member / Haroldo Amorim de Almeida - Member / Bruno Pareira Alves - Member / Carmem Gilda Barroso Tavares Dias - Member / Hilton Túlio Costi – Member
Funding: Paraense Amazon Foundation for Research Support - Financial Aid
Number of counselings: 11
2008 - 2011
Evaluation of the Knoop Microhardness, Flexural Strength and Degree of Monomeric Conversion of Composite Resins Photoactivated by Different Sources Light Source and Photoactivation
Description: Approved by the Official Notice 003/2008 – Technology and Innovation Research Project - Universal.
Status: In progress
Nature: Research
Students involved: Undergraduate Courses: (2)
Members: Cecy Martins Silva - Member / Jesuína Lamartine Nogueira Araújo - Member / Armando de Brito Chermont - Member / Eliane Bemerguy Alves - Coordinator / Carmem Gilda Barroso Tavares Dias – Member
Funding: Paraense Amazon Foundation for Research Support - Financial Aid
2004 - 2007
In Vitro Study of the Color Change of a Composite Resin Photoactivated by Different Sources of Light When Undergoing the Action of Coffee and Acai Associated or Not with Alcoholic Solutions
Description: Project approved by CNPq (Universal Notice)
Status: Completed
Nature: Research
Students involved: Undergraduate Courses: (1)
Members: Cecy Martins Silva - Member / Jesuína Lamartine Nogueira Araújo - Member / Eliane Bemerguy Alves - Member / Kátia Regina Hostílio Cervante Dias - Member / Hervé rogez - Coordinator.
Funding: National Council for Scientific and Technological Development - Financial Aid
Number of S, T & A Productions: 5
Number of counselings: 1
2002 - 2003
Microbiological Study of the Efficiency of Disinfectant Solutions in Alginate Molds
Description: Scientific Research Project approved by the Research Support Program - PIBIC.
Status: Completed
Nature: Research
Members: Cecy Martins Silva - Member / Eliza Burlamaqui Klautau - Coordinator / Renata Antunes Esteves - Member / Maria das Graças Carvalho de Almeida - Member.
Funding: Centro Universitário do Pará - Financial Aid
2002 - 2003
In vitro evaluation of Marginal Infiltration in Class V Cavity Restored with Flow Resin Isolate and Associated with Different Types of Composite Resin
Description: Project approved by the Research Support Program - CESUPA.
Status: Completed
Nature: Research
Students involved: Undergraduate Courses: (2)
Members: Cecy Martins Silva - Coordinator / Sandra Chaves Daher - Member / Vaneska Galindo – Member
Number of S, T & A Productions: 1
Number of counselings: 1
2002 - 2002
Comparative Study of Color Change of Different Trademarks of Photopolymerizable Microhybrid Composite Resins by Exposure to Drink Containing Dye
Description: Research Project Approved by PROPESP-UFPA, PPQ-CCS-0041/2002. Coordinator: Cecy Martins Silva.
Status: Completed
Nature: Research
Students involved: Undergraduate Courses: (2)
Members: Cecy Martins Silva - Coordinator / Eliza Burlamaqui Klautau - Member.
Funding: Federal University of Pará - Financial Aid / Cento Universitário do Pará - Financial Aid
Number of S, T & A Productions: 3
2002 - 2002
Evaluation of the Prevalence of Abfraction Associated or not with Dentinary Hyperesthesia in Patients with Paraprofessional Habits
Description: Scientific Research Project approved by the Research Support Program - PIBIC.
Status: Completed
Nature: Research
Students involved: Undergraduate Courses: (2)
Members: Cecy Martins Silva - Coordinator / Eliza Burlamaqui Klautau - Member / Renata Antunes Esteves – Member
Funding: CESUPA - Financial Aid
Number of S, T & A Productions: 4
Number of counselings: 2
2000 - 2002
Evaluation, Rehabilitation and Control of Oral Health of Geriatric Patients Residing in the Municipality of Belém-PA
Description: Project approved by the Integrated Program to Support Teaching, Research and Extension – PROINT No. 0131/2000, Carried out by the UFPA’s Dentistry Undergraduate Course.
Status: Completed
Nature: Research
Students involved: Undergraduate Courses: (2)
Members: Cecy Martins Silva - Member / Regina Fátima Feio Barroso - Coordinator.
Funding: Federal University of Pará - Financial Aid
Number of S, T & A Productions: 6
Number of counselings: 4
1999 - 2001
Evaluation of Oral Health of Patients Dependent on Chemical Drugs
Description: Project carried out by the UFPA’s Dentistry Undergraduate Course.
Status: Completed
Nature: Research.
Students involved: Undergraduate Courses: (2)
Members: Cecy Martins Silva - Member / Eliane Berneguy Alves - Member / Regina Fátima Feio Barroso – Coordinator
Funding: Federal University of Pará - Financial Aid
Number of counselings: 2
1998 - 1999
Diagnosis of Oral Health in Families of Rural Producers in the Municipality of Mojú – PA
Description: Project Code: PPQ - CCS - 003/1998.
Status: Completed
Nature: Research
Students involved: Undergraduate Courses: (2)
Members: Cecy Martins Silva - Coordinator / Maria de Nazaré Carvalho Almeida - Member / Regina Fátima Feio Barroso - Member / Claudionor Rodrigues Pimentel – Member
Funding: State Office for the Environment – Financial Aid / State Office for Science, Technology and the Environment – Financial Aid
Number of S, T & A Productions: 1
Number of counselings: 1
1997 - 1998
Agroenvironmental Diagnosis of Rural Properties of Agricultural Border Areas of the State of Pará – Mojú
Description: Interinstitutional and Multidisciplinary Project. Institutions Involved: EMBRAPA-CPATU (Coordination); UFPA; SESPA; EMATER; IDESP Approved by the Science and Technology State Fund – FUNTEC and State Office for Science and Technology – SECTAM.
Status: Completed
Nature: Research
Students involved: Undergraduate Courses: (2)
Members: Cecy Martins Silva - Member / Regina Fátima Feio Barroso - Member / Luiz Guilherme Teixeira silva – Coordinator
Funding: Studies and Projects Funder – Financial Aid
Number of S, T & A Productions: 1
Number of counselings: 1
2346057 - Fabricio Mesquita Tuji (562.989.272-04)
2611325 - Gustavo Antonio Martins Brandao (686.252.432-15)
2449316 - Helder Henrique Costa Pinheiro (635.878.232-00)
2017 - Current
Neglected Diseases in the State of Pará: Temporal and Spatial Analysis
Description:
Neglected diseases have common characteristics of high endemicity in the rural and less-favored urban areas of developing countries, as well as the scarcity of research for the development of new drugs. These diseases can hamper child growth and intellectual development as well as labor productivity. Thus, neglected diseases are those that do not present economic attractiveness for the development of drugs, either because of their low prevalence or because they reach low-level populations (ANVISA, 2007). In this sense, they not only occur more frequently in impoverished areas, but also are conditions that promote poverty (HOTEZ et al., 2006). In this scenario, the current study is justified in order to analyze the records of neglected diseases to verify their epidemiological nature and, consequently, to contribute to the epidemiological and sanitary surveillance services of the State of Pará and its Municipalities. The objective of this study was to analyze the epidemiological characteristics of records of detected cases of neglected diseases in the State of Pará, in temporal and spatial perspectives. The Project was approved and registered with the Institute of Health Sciences under Ordinance No. 327/2017, and was approved in Propesp / UFPA Notice 05/2017, under the Researcher Support Program (PRODOUTOR).
Status: In progress
Nature: Research.
Students involved: Undergraduate Courses: (6) / Academic Master’s: (1)
Members: Helder Henrique Costa Pinheiro - Coordinator / Marizeli Viana de Aragão Araújo – Member
Funding: Federal University of Pará - Scholarship
2017 - Current
Oral Health Conditions of Workers Served in the Dental Service of the Social Service of Commerce – SESC
Description:
Health surveillance, identification and reduction of risks, has become the central strategy of health services actions focused on promotion (CZERESNIA, 2004), including worker health. The dentistry of work is the dental specialty that has the attribution of promoting, preserving and rehabilitating the oral health of workers and verifying (NOGUEIRA, 1972). For this, the oral health team needs to identify target populations and their characteristics based on an epidemiological survey of the workers through dental examinations and analyzes of their medical records (BARROS; DIAS; MELLO, 2006). The characterization of a population of workers in relation to oral health is necessary for the identification of the oral health conditions of individuals who demand dental care for health services, such as the Dental Service of the Social Service of Commerce (SESC) State of Pará. The epidemiological analysis collaborates with information about what are the main problems that the workers' public presents in their initial dental examination and the return examinations for maintenance of oral health. This analysis can also guide the management of assistance resources to meet the needs that trade sector workers most frequently present. The objective was to analyze the oral health conditions of workers assisted by the Social Service of Commerce – SESC / Pará. The project was approved and registered with the Health Sciences Institute under Ordinance No. 356/2017.
Status: In progress
Nature: Research.
Students involved: Undergraduate Courses: (6) / Academic Master’s: (1)
Members: Helder Henrique Costa Pinheiro - Coordinator / Marizeli Viana de Aragão Araújo - Member.
2017 - Current
Prevalence of Epstein-Barr Virus, Cytomegalovirus and Human Papillomavirus in Periodontal Disease
Project certified by Coordinator Marizeli Viana de Aragão Araújo on 07/18/2017.
Description: The implication of the Epstein-Barr virus (EBV), cytomegalovirus (CMV) and human papillomavirus (HPV) in the development of periodontal disease has been highlighted. Carrying out studies to try to elucidate the relationship of these viruses with periodontal disease becomes important because of the possibility of performing a correct and early diagnosis avoiding losses that reflect on the quality of life of the individuals carrying this disease. In this sense, the study aims to verify the prevalence of EBV, CMV and HPV in the gingival sulcus of individuals affected by periodontal disease and in individuals with periodontal health. The Project was approved and registered in the Institute of Health Sciences under Ordinance No. 243/2017 and was contemplated in Propesp/UFPA Official Notice No. 06/2017 in the Institutional Program of Scientific Initiation and Technological Development and Innovation Scholarship of UFPA (PIBIC-UFPA), with a PIBIC / CNPq scholarship.
Status: In progress
Nature: Research.
Students involved: Undergraduate Courses: (2) / Academic Master’s: (1).
Members: Helder Henrique Costa Pinheiro - Member / Marizeli Viana de Aragão Araújo – Coordinator
Funding: National Council for Scientific and Technological Development – Scholarship
2017 - Current
Temporal and Spatial Analysis of Viral Hepatitis in the State of Pará
Description:
In February 2002, the Ministry of Health created the National Program for the Prevention and Control of Viral Hepatitis (PNHV – Portuguese acronym), which was integrated into the Epidemiological Surveillance Department Office (DEVEP) of the Office of Health Surveillance (SVS) (BRASIL, 2005) in October 2003. Among its objectives is to strengthen epidemiological and sanitary surveillance in the control of hepatitis in the population. It is known that the lack of reliable information can compromise both the formulation of health indicators and epidemiological studies and the implementation of measures to improve the standard of living of the population (MASCARENHAS; GOMES, 2011). However, the information obtained from the notifications allows the spatial-temporal monitoring of epidemics in the country, subsidizing the actions for their prevention and control (CERQUEIRA et al, 2011). In this scenario, the present study is justified in order to analyze the viral hepatitis registries to verify their epidemiological nature and, consequently, to contribute to the epidemiological and sanitary surveillance services of the State of Pará and its Municipalities. The objective of the present study is to analyze the epidemiological characteristics of the records of detected cases of viral hepatitis in the State of Pará, in temporal and spatial perspectives. The Project was approved and registered at the Institute of Health Sciences under Ordinance No. 357/2017.
Status: In progress
Nature: Research.
Students involved: Undergraduate Courses: (6) / Academic Master’s: (1)
Members: Helder Henrique Costa Pinheiro - Coordinator / Marizeli Viana de Aragão Araújo – Member
2017 - Current
Evaluation of Health Technologies in the Amazonian Context
Description: To develop technical-scientific reports and systematic review studies to evaluate health technologies. The technologies evaluated are of interest to the dental clinic and especially those of application in collective oral health under the Unified Health System (SUS) in the Amazon context.
Status: In progress
Nature: Research.
Students involved: Undergraduate Courses: (12) / Academic Master’s: (4)
Members: Helder Henrique Costa Pinheiro - Coordinator / Marizeli Viana de Aragão Araújo - Member / Regina Fátima Feio Barroso - Member / Danielle Tupinambá Emmi - Member / Ana Daniela Silva da Silveira - Member / Antônio David Corrêa Normando - Member / Rafael Rodrigues Lima – Member
2017 - Current
Surveillance in Collective Oral Health in the Amazon
Description:
To develop studies of Public Collective Health Surveillance in the territory of the Amazon in the areas of Epidemiological Surveillance (oral problems of public health interest), Sanitary Surveillance (monitoring fluoridation of public water supply and products and services used individually by the population that affect oral health), Environmental Surveillance (in the control of the fluoride concentration in waters of natural sources of human consumption) and Worker Health (impact of the labor activities of the various productive systems on the oral health of workers).
Status: In progress
Nature: Research.
Students involved: Undergraduate Courses: (10) / Academic Master’s: (4)
Members: Helder Henrique Costa Pinheiro - Coordinator / Marizeli Viana de Aragão Araújo - Member / Regina Fátima Feio Barroso – Member / Danielle Tupinambá Emmi - Member / Ana Daniela Silva da Silveira - Member / Antônio David Corrêa Normando - Member / Rafael Rodrigues Lima – Member
2013 - 2016
Coverage and Surveillance of Fluoridation of Public Water Supply in Brazil
Description:
To describe the population coverage of water fluoridation of the public water supply system in municipalities with more than 50 thousand inhabitants in the first half of the second decade of the 21st century and to identify the localities that have been carrying out monitoring activities of this measure in the municipal and state spheres. In a complementary way, the fluoride concentration in selected localities will be analyzed, in order to confirm or know the content of halogen present in public water supply for human consumption.
Status: Completed
Nature: Research
Students involved: Academic Master’s: (2) Doctorate: (2)
Members: Helder Henrique Costa Pinheiro - Member / Paulo Frazão - Coordinator / Paulo Capel Narvai - Member / Jaime Aparecido Cury - Member / Tiago Araújo Coelho de Souza - Member / Marco Antônio Manfredini - Member / Luiz Roberto Noro - Member / Helenita Corrêa Ely – Member
2011 - 2013
Cariology and the Amazon: the trace elements in food and the process of formation of dental caries
Description:
Project in the area of Collective Health (Cariology), Researching the fruits of the Amazon (Brazil Nut, Bacuri, Pupunha and Tucumã), in the proposal to analyze them as protective foods in the process of dental caries formation. Ordinance No. 061/2011-ICS rectified by Ordinance No. 122/2011-ICS, which was rectified by Ordinance No. 0345/2011, with an allocation of 05 hours of work per week.
Status: Completed
Nature: Research
Students involved: Undergraduate Courses: (2) / Academic Master’s: (2) / Doctorate: (2)
Members: Helder Henrique Costa Pinheiro - Coordinator / Marizeli Viana de Aragão Araújo - Member / Regina Fátima Feio Barroso - Member / Danielle Tupinambá Emmi - Member / Tamea Lacerda Monteiro - Member / Etiane Prestes Batirola – Member
2011 - 2012
Analysis of the Oral Health Conditions of Individuals with Sickle Cell Anemia at the HEMOPA Foundation
Description:
The aim of the Research Project is to know the oral health conditions of individuals with sickle cell disease treated at the HEMOPA Foundation, aiming to estimate the prevalence and severity of dental caries, dental treatment needs and periodontal condition, as well as to investigate the susceptibility of these individuals to oral problems and to analyze self-care and self-perception of oral health. The study has a Transversal design, with an Observational and Analytical character, through an Epidemiological Survey based on oral exams and surveys with the study subjects. To register the oral problems, we used the Dental Caries and Treatment Need Index and the Community Periodontal Index and a questionnaire was developed to obtain information on socioeconomic status and self-perception of oral health. A Free and Informed Consent Form was also prepared to be obtained with all the individuals inspected, and the Project obtained a favorable opinion for its accomplishment by the Research Ethics Committee of the Hematology and Hematology Center of Pará - HEMOPA under No. 00006.0.324.324-11. Ordinance No. 302/2011 ICS / UFPA: Project approval until December 2012, allocating a workload of 5 hours per week.
Status: Completed
Nature: Research.
Students involved: Undergraduate Courses: (4) / Academic Master’s: (2)
Members: Helder Henrique Costa Pinheiro - Coordinator / Alda França Costa - Member / Marizeli Viana de Aragão Araújo - Member / Regina Fátima Feio Barroso - Member / Danielle Tupinambá Emmi - Member / Ana Daniela Silva da Silveira - Member / Liliane Silva do Nascimento - Member.
Number of counselings: 1
2010 - 2012
Multicentric Study of Demography and Health in the Marajó Archipelago (EMDSAM)
Description:
This project was recently approved in the scope of Notice 023/2009 – FAPESPA / PPSUS. It obtained a financial contribution of R$ 1,800,000.00 (one million, eight hundred thousand reais) for the development of the proposal in a period of two years. It is among 33 specific objectives with the participation of several Research Institutions and Teaching under Coordination of the Evandro Chagas Institute. Approval of the Institute of Health Science / UFPA under Ordinance No. 198/2010-ICS.
Status: In progress
Nature: Research
Students involved: Undergraduate Courses: (2) / Academic Master’s: (2)
Members: Helder Henrique Costa Pinheiro - Coordinator / Regina Fátima Feio Barroso - Member / Danielle Tupinambá Emmi - Member / Ana Daniela Silva da Silveira – Member
Funding: Paraense Amazon Foundation for Research Support - Financial Aid / National Council for Scientific and Technological Development - Financial Aid
2010 - 2011
Analysis of Oral Health Conditions in Pregnant Women in the Municipality of Benevides
Description:
The objective of the study is to analyze the occurrence of dental caries, periodontal disease, use and need for prosthesis in addition to the oral health attitudes and practices of pregnant women assisted by the "Santos Dumont" Family Health Team of the Municipality of Benevides / PA.
Status: In progress
Nature: Research
Students involved: Undergraduate Courses: (1)
Members: Helder Henrique Costa Pinheiro - Coordinator / Alda França Costa - Member / Regina Fátima Feio Barroso - Member / Danielle Tupinambá Emmi - Member / Ana Daniela Silva da Silveira - Member / Elder Dioney Noronha Ferreira – Member
2010 - 2010
Hepatitis C in the Perception of the Undergraduates of the Dentistry Courses in Belém-PA
Description:
The objective of this study was to verify the knowledge of Undergraduates of Dentistry courses in the city of Belém / PA on Hepatitis B and C and its prevention methods. Approval of the Project by the Institute of Health Sciences / UFPA under Ordinance No. 149/2010.
Status: Completed
Nature: Research
Students involved: Undergraduate Courses: (2)
Members: Helder Henrique Costa Pinheiro - Member / Alda França Costa - Member / Marizeli Viana de Aragão Araújo - Member / Regina Fátima Feio Barroso - Member / Danielle Tupinambá Emmi - Member / Ana Daniela Silva da Silveira – Coordinator
2009 - 2011
Oral Health in the Island of Marajó: diagnosis of the problems and proposal of the implementation of public policies of Dentistry
Description:
Project submitted to the Official Notice No. 13/2009 MS / FAPESPA / CNPq and approved with financing of R$ 113,184.50. Ordinance No. 049/2011-ICS / UFPA of Inclusion of Professors in the Project, rectified by Ordinance No. 131/2011-ICS / UFPA.
Status: Completed
Nature: Research
Students involved: Undergraduate Courses: (2) / Academic Master’s: (1)
Members: Helder Henrique Costa Pinheiro - Member / Alda França Costa - Member / Marizeli Viana de Aragão Araújo - Member / Ceci Baker de Melo - Member / Regina Fátima Feio Barroso - Coordinator / Maria Sueli da Silva Kataoka - Member / João de Jesus Viana Pinheiro – Member
Funding: Paraense Amazon Foundation for Research Support – Financial Aid
1152832 - Joao de Jesus Viana Pinheiro (257.152.672-34)
2017 - Current
Expression of Invadapod-Related Proteins and Their Role in In Vitro Invasivity of Odontogenic Keratocyst Cell Line
Description:
Odontogenic keratocyst (CO – Portuguese acronym) is a benign odontogenic neoplasia, although it presents aggressive behavior and a high rate of recurrence. Recent studies associate the pathogenesis and development of CO to the epithelial-mesenchymal transition process, a condition in which changes in the cohesion, polarization and mobility of oral epithelial cells occur. In order to better understand the biological behavior of these neoplastic cells and their morphology, the objective of this work is to verify the expression of invadopodal proteins and the in vitro study of the invasiveness of a CO cell line. For this, a CO lineage will be cultured and expression experiments of the TKS-4, TKS5, MMP14 and Cortactin proteins will be performed. Interference RNA for TKS-4 and TKS-5 will also be performed to highlight the possible relationship between cell invasion and invadopod.
Status: In progress
Nature: Research.
Students involved: Undergraduate Courses: (3) / Academic Master’s: (2)
Members: João de Jesus Viana Pinheiro - Coordinator / Maria Sueli da Silva Kataoka - Member / Sergio de Melo Alves Jr. - Member / Sâmia Cordovil de Almeida - Member / Geovanni Pereira Mitre - Member / Giordanna Pereira Chemelo - Member / Elizeu da Silva Botelho - Member / Karina Pereira Rosa – Member
2017 - Current
In Vitro Expression of Proteins TKS4, TKS5, MT1-MMP and Cortactin Related to Invadopod Formation and the Process of Invasion in the Odontogenic Tumor Ameloblastoma
Description:
Ameloblastoma (AME) is a benign odontogenic tumor of epithelial origin that presents slow growth, locally invasive, high rates of recurrence and is subdivided into three variants: unicystic, multicystic and peripheral / extraosseous. Recent studies elucidate the mechanisms associated with cellular invasiveness to characterize the tumor microregions of this neoplasm, revealing that localized proteolysis is mediated by invadopodes, which are cell-membrane protrusions that have pericellular proteolytic activity. In order to better understand the biological behavior of these neoplastic cells and their morphology, the objective of this work is to verify the expression of proteins related to the invadopodium and its participation in the invasion process. By means of immunofluorescence, new studies can be carried out allowing a better understanding of cellular events important for neoplastic progression.
Status: In progress
Nature: Research
Students involved: Undergraduate Courses: (3) / Academic Master’s: (2)
Members: João de Jesus Viana Pinheiro - Coordinator / Maria Sueli da Silva Kataoka - Member / Sergio de Melo Alves Jr. - Member / Sâmia Cordovil de Almeida - Member / Geovanni Pereira Mitre - Member / Giordanna Pereira Chemelo - Member / Elizeu da Silva Botelho - Member / Karina Pereira Rosa – Member
2016 - Current
UCL´s Global Engagement Funds
Description: Odontogenic tumours constitute a group of neoplasms that range from benign non-aggressive to malignant tumours with metastatic potential. Ameloblastoma and keratocystic odontogenic tumours (KOT) are highly prevalent and aggressive odontogenic tumors. The recommended treatment is local resection, which results in considerable morbidity and extensive reconstructive surgery to the jaw. Due to the excessive costs and surgical proficiency associated with reconstructive surgery, non-developed countries implement alternative less affective treatments. These include marsupialization and enucleation with/without adjuvant treatment (liquid nitrogen, Carnoy´s solution, peripheral ostectomy). Marsupialization is a conservative surgical technique that reduces the intralesional pressure by exposing the lumen of the tumour to the oral cavity. This treatment is believed to benefit the patient in two ways: 1- removal of intralesional pressure and; 2- exposition of the neoplastic cells to the oral bacteria. Exposure of the tumor to the oral bacteria induces an inflammatory response, which is believed to aid the anti-tumor response. We would like to investigate the tumors response to immune stimulation, in regards to activity, viability and invasiveness. Gaining a better understanding of how these tumors respond to immune stimulation may facilitate the development of adjunct therapies, which will have the potential to improve the effectiveness of marsupialization. My colleagues in Brazil have established two stable human cell lines derived from ameloblastoma and KOT. We intend bringing these cells to my laboratory and study the cellular responses to a range of immune stimulants.
Status: In progress
Nature: Research
Students involved: Undergraduate Courses: (2) / Academic Master’s: (3) / Doctorate: (1)
Members: João de Jesus Viana Pinheiro - Member / André Luis Ribeiro Ribeiro - Member / Andrew M. Smith – Coordinator
Funding: University College London - Financial Aid
2015 - Current
Tissue, Biochemistry and Proteomics Evaluation of Salivary Glands in Animal Models of Exposure to Metals
Project certified by Coordinator Rafael Rodrigues Lima on 12/27/2017.
Description:
This project aims to evaluate the effects of systemic exposure to potentially toxic metals such as mercury, aluminum, lead and cadmium, common in mining or mineral exploration areas in the Amazon. The mining activity, legal and clandestine, together with the waste dumped in the rivers from the large mining companies make the Amazon region a region where toxicological damage from exposure to metals can be considered endemic, being one of the greatest challenges in public health. The objective is to simulate the toxicological damages caused by environmental exposure to rodent salivary glands. For this purpose, a variety of toxicological evaluation parameters will be used, from the measurement of metal deposits in the glandular parenchyma, analysis of oxidative biochemistry, proteomic evaluation and tissue / immunohistochemical characterization of the reaction and / or tissue components damage in salivary glands.
Status: In progress
Nature: Research
Students involved: Undergraduate Courses: (2) / Academic Master’s: (1)
Members: João de Jesus Viana Pinheiro - Member / Sérgio de Melo Alves-Junior - Member / Rafael Rodrigues Lima – Coordinator
2014 - Current
Mecanismos de Invasão Celular no Ameloblastoma “Cross Talk” entre NOTCH1, ADAM-12, HIF-1α e HB-EGF
Description:
Ameloblastoma is a benign neoplasm characterized by local invasiveness and high rates of recurrence. The tumor microenvironment is an important regulator of invasive behavior and its study can help elucidate the molecular mechanisms that permeate the progression of neoplasias and in the determination of markers that allow a better design in the treatment of tumors. It is known that the concentration of oxygen in the microenvironment around the neoplastic cells is reduced, resulting in intratumoral hypoxia. Hypoxia was associated with increased metastasis and neoplastic invasion, besides potentiating the formation of invadopodia, which are cell membrane protrusions rich in actin and endowed with intrinsic proteolytic activity. Under conditions of hypoxia, the proteins NOTCH1, ADAM-12, HIF-1α and HB-EGF, together, have been related to the formation of invadopodia. In this condition, HIF-1α levels are stabilized and NOTCH1 signaling is activated by increasing the level of the JAG2 linker by recruiting the γ-secretase that will cleave NOTCH1 and release its intracellular domain. This intracellular domain, translocated to the nucleus, will increase levels of ADAM-12, whose activity promotes the cleavage of the pro-HB-EGF domain, releasing HB-EGF into the extracellular region.
HB-EGF will bind to EGFR, located on the plasma membrane of both the cell that released it and adjacent cells. These autocrine and paracrine signaling lead to the formation of invadopodia, and may additionally stimulate invadopodia in cells located in normoxic regions, which suggests cross-signaling between the areas of normoxia and tumor hypoxia. This signaling model clearly demonstrates the interrelationship between the proteins we propose to study in this project. Thus, our goal in this project is to analyze the expression of the proteins NOTCH1, ADAM-12, HIF-1α and HB-EGF in the ameloblastoma, to better understand the initial phases of the process of tumor invasion related to hypoxia. In the first phase of the project, in vivo studies will be performed, using immunohistochemistry, in order to identify if there is expression and correlation of the proteins studied in samples of ameloblastoma. We will verify the expression of these proteins in human ameloblastoma cell line (AME-hTERT) using immunofluorescence and transcriptomic analysis. Using an in vitro model, we will analyze invasion and invadopodia formation under conditions of hypoxia and normoxia. Additionally, by depleting the expression of NOTCH-1 by RNAi, we will evaluate cell invasion and invadopodia formation under conditions of hypoxia and normoxia. We believe that these data may bring new information that may help in controlling the invasive spread of this neoplasm.
Status: In progress
Nature: Research.
Students involved: Undergraduate Courses: (3) / Academic Master’s: (4)
Members: João de Jesus Viana Pinheiro - Coordinator / Vanessa M. Freitas - Member / Ruy Gastaldoni Jaeger - Member / Maria Sueli da Silva Kataoka - Member / Sergio de Melo Alves Jr. - Member / Adriane Sousa de Siqueira - Member / João Rafael Habib Souza Aquime - Member / Lara Carolina D?Araújo Pinto - Member / Jéssica de Almeida Lopes - Member / Natacha Malu Miranda da Costa - Member / Artur Luiz da Silva - Member / Giordanna Pereira Chemelo - Member / Elizeu da Silva Botelho - Member / Karina Pereira Rosa - Member.
Funding: National Council for Scientific and Technological Development - Financial Aid
Number of S, T & A Productions: 15
Number of counselings: 1
2013 - Current
C 16 Peptide, Laminin Derivative, Regulating the Expression of Potential Breast Cancer Biomarkers
Project certified by Coordinator Ruy Gastaldoni Jaeger on 12/22/2013.
Description:
Breast cancer represents an important public health problem. The microenvironment where the neoplastic cells are found plays an important role in tumorigenesis and tumor progression. Our Laboratory studies the role of laminin and its bioactive peptides in tumor biology. We verified that the C16 peptide, derived from laminin-111, regulates the differential gene expression in breast tumors (DE SOUZA SANTOS, 2011). The C16 peptide, present in the short arm of the gamma 1 chain, stimulates the expression of GPNMB and SPOCK1 genes. These results were obtained in the breast tumor line MDA-MB-231. In this project, we will evaluate the action of the C16 peptide on the expression of the molecules encoded by the GPNMB and SPOCK1 genes, which in addition to regular cell adhesion, migration and metastasis may be potential tumor biomarkers. Thus, we will use breast cancer strains with different behaviors, such as MBA-MB-231 (basal-like, triple negative for estrogen, progesterone and HER2 receptors) and MCF-7 (luminal B HER2 positive). For comparison purposes we will use normal breast cells (MCF-10A). The three lines will be treated by C16, the expression of GPNMB and SPOCK1 being analyzed by immunoblot. To correlate protein expression and cellular function, we will investigate whether the C16 peptide would induce invasiveness of the MDA-MB-231 and MCF-7 tumor lines. Additionally, through blocking of function by antibodies, we will analyze if GPNMB and SPOCK1 would be related to the invasion possibly triggered by the peptide. Finally, in order to contextualize our in vitro results in vivo, we will study the potential role of GPNMB and SPOCK1, as tumor biomarkers, through the immunodetection of these molecules in normal and tumoral mammary tissue, using Tissue Microarrays (TMAs). We will try to correlate the expression profile of these molecules with clinical data of patients, including staging and survival curve.
Status: In progress
Nature: Research.
Students involved: Academic Master’s: (2) Doctorate: (1)
Members: João de Jesus Viana Pinheiro - Member / Vanessa M. Freitas - Member / Ruy Gastaldoni Jaeger - Coordinator / Siqueirra AS - Member / Emerson S Santos - Member / Basílio Smuczek - Member / Lívia Caires dos Santos - Member.
Funding: National Council for Scientific and Technological Development – Financial Aid
2012 - Current
In Vitro Study of the Relationship between Metallothionein Expression, Matrix Metalloproteinases, Growth Factors and Events of Cell Proliferation in Adenoid Cystic Carcinoma
Estudo in Vitro da Relação entre a Expressão de Metalotioneína, Metaloproteinases da Matriz, Fatores de Crescimento e Eventos de Proliferação Celular no Carcinoma Adenoide Cístico
Description:
Cystic adenoid carcinoma (CAC) is a malignant neoplasm that mainly affects the minor salivary glands, accounting for about 10% to 15% of all tumors in the head and neck region (Neville et al., 2009, Khan et al. 2001, NAMAZIE et al., 2001). This neoplasm often gives rise to slow, painless but highly invasive lesions with high recurrence rates, distant metastasis and mortality (KHAFIF et al., 2005). Histologically, it is characterized by showing epithelial and myoepithelial cells arranged in histological patterns called cribiform, tubular and solid (PEREZ et al., 2006). Many studies suggest that each of the histological subtypes of CAC is related to different clinical behaviors (SZANTO et al., 1984, ALVES et al., 2007, WGI et al., 2005). Tumor invasion inherent to CAC is a complex process mediated, among other factors, by cell proliferation and localized proteolysis of the extracellular matrix, mainly performed by enzymes called matrix metalloproteinases (MMPs). Among these proteases, MMP-2 and -9 have a prominent role in the invasiveness of tumors, since they degrade several components of the matrix, such as collagen (STERNLICHT, WERB, 2001; ZHANG et al., 2009). It is well known that MMPs are zinc dependent endopeptidases to exert their enzymatic function. (BIRKEDAL-HANSEN et al., 2003). Thus, the phenomena related to MMPs can occur with the help of metallothionein, a protein that functions as an intracellular reservoir of zinc ions for the activation of these proteases, as well as certain transcription factors. A study of the expression of metallothionein in ameloblastoma suggests not only its pro-mitotic and antiapoptotic role, but also its function as a reservoir of zinc ions for important proteases (RIBEIRO et al., 2011, Cing, 2011).
Status: In progress
Nature: Research
Students involved: Undergraduate Courses: (2) / Academic Master’s: (2) / Doctorate: (1)
Members: João de Jesus Viana Pinheiro - Member / Ruy G Jaeger - Member / Maria Sueli da Silva Kataoka - Member / José Antônio Picanço Diniz Junior - Member / Sergio de Melo Alves Jr. - Coordinator / Adriane Sousa de Siqueira - Member / Aline Semblano Dias Carreira - Member / Marina Rolo Pinheiro da Rosa - Member / João Rafael Habib Souza Aquime - Member / Lara Carolina D'Araújo Pinto - Member.
Funding: National Council for Scientific and Technological Development – Financial Aid
2012 - Current
Indicators of Oral Health in Indigenous People of the Xingu Middle Valley
Indicadores da Saúde Bucal em Indígenas do Vale Médio do Xingu
Description:
Census data showed an average annual growth rate of 10.8% among individuals who declared themselves to be indigenous between the 1991 and 2000 censuses. However, in parallel with the growth scenario, there is an increase in the process of acculturation of Brazilian natives. Data from IBGE (2005) show that, in 2000, about 50% of the indigenous population lived in urban centers. This contact with the non-indigenous population may lead to an increase in oral diseases as a consequence of the miscegenation process, as a result of the increase in consumption of industrialized products, including sugar and the loss of cultural and morphological characteristics, even before they are duly identified. The scientific literature presents a few studies on the epidemiological characteristics of oral health conditions and biometric data on the morphofunctional characteristics of the Brazilian natives' dentition and face. In addition, there are no reports on the effects of miscegenation, resulting from the processes of fission and fusion of indigenous groups and loss of identity through contact with non-Indians in oral health. Regarding the indigenous populations of the middle Xingu valley, there is also a recent increase in migratory flows due to the construction of the Belo Monte hydroelectric plant in the region known as the great Xingu.
Such studies are crucial to define important epidemiological aspects related to the characterization of these populations, as well as the understanding of how the anthropomorphic Indian traits contributed to the formation of the Brazilian population, notoriously mixed. In this perspective, the universal contribution that the study of relatively isolated populations can give to the understanding of the influence of the habits and customs of modern man in the etiology of oral diseases. Thus, the objective of this research is to investigate oral health indicators and possible associated etiological factors by conducting a wide survey of the occurrence of oral diseases in the indigenous populations of the middle valley of the Xingu River and its affluents Iriri and Bacajá. Biometric studies on the dentofacial characteristics of these indigenous groups will also be carried out. The sample of the present study will be collected from the examination of the entire indigenous population allocated in the Altamira Indigenous Special Sanitary District (DSEI – Portuguese Acronym). This district, with its head office in the municipality of Altamira-state of Pará-Brazil, is composed of 2,141 indigenous people, from 16 villages, belonging to 3 linguistic groups and 9 ethnicities. This scientific research will contribute greatly to the implementation of basic care programs, based on the family health strategy, meeting the specific needs of each population group. Also, the benefits of acquiring scientific knowledge about the anthropomorphic characteristics of the Amazonian indigenous populations are highlighted.
Status: In progress
Nature: Research.
Students involved: Undergraduate Courses: (2) / Academic Master’s: (2)
Members: João de Jesus Viana Pinheiro - Member / Regina Fátima Feio Barroso - Member / Isabela Almeida Pordeus - Member / Ana Cláudia Braga Amoras Alves - Member / Antônio David Corrêa Normando - Coordinator / Alberto Fábio Pereira Lima - Member / Cátia Cardoso Abdo Quintão - Member / Liliane Silva do Nascimento - Member / Saul Martins de Paiva – Member
Funding: Paraense Amazon Foundation for Research Support - Financial Aid
2012 - Current
Application of Amazonian Herbal Medicine in Oncology
Description:
One of the most frequent oral complications of chemotherapy is mucositis, a change in inflammatory character for which there is no definite treatment yet. This oral complication causes severe discomfort and pain, makes feeding difficult, and may result in discontinuation of the antineoplastic treatment. Considering the clinical relevance of mucositis, it is important to find ways to treat it. Carapa Guianensis Aubl, popularly known as “Andiroba” has active principles that gives this phytotherapeutic potential anti-inflammatory, analgesic and antiallergenic. This set of characteristics aroused the attention to study the action of Andiroba in the treatment of oral mucositis (OM) symptomatology in patients on cancer treatment. This Research Line aims to evaluate the potential action potential of Andiroba (Carapa Guianensis Aubl) on two fronts. In the healing of oral mucositis induced by 5-fluorouracil (5-FU) in hamsters, through clinical, histopathological and immunohistochemical evaluation and in cancer patients submitted to chemotherapy.
Status: In progress
Nature: Research
Students involved: Undergraduate Courses: (2) / Academic Master’s: (1)
Members: João de Jesus Viana Pinheiro - Member / Fabrício Mesquita Tuji - Coordinator / Sergio de Melo Alves Jr. – Member
2010 - 2013
Isolation and Characterization of Ameloblastoma-Derived Cells. In Vitro Study of Modulation of Matrix Metalloproteinases by Growth Factors
Description: This project will enable the implementation of new technologies in the undergraduate and graduate courses in Dentistry at the Federal University of Pará, resulting, therefore, in the fulfillment of what is recommended in the recent initiatives of the research development agencies of the country, regarding the greater integration of educational institutions, in terms of research and graduate studies, given the need to consolidate research in emerging institutions and, consequently, its Graduate Programs, which depends on the qualification of its professors and technicians. The development of tumor cell lines is a procedure that has been used by several researchers in order to better understand the biological behavior of the most varied neoplasms in vitro, as well as to contribute to the advances in the prognosis of the lesion, diagnosis and therapeutics. The methods used will allow the characterization of recent isolation of tumor parenchyma and stroma cells, which is believed to be a primary cell line originated from an Ameloblastoma. This method, therefore, will allow the execution of more mechanistic studies where, using gene silencing, we hope to correlate the growth factors with the synthesis of MMPs. According to the established correlation, an important step has been taken in elucidating the way in which growth factors can modulate the release of MMPs, their possible interactions with TIMPs and the participation of these growth factors with remodeling and invasion site of this neoplasm. On the other hand, several factors can alter normal cell regulation affecting the function of the genes that control cell division and repair, apoptosis, and angiogenesis. Mutations in DNA may include large rearrangements such as translocations, inversions, amplifications and deletions of genes, as well as nucleotide mutations with insertions and deletions of bases.
Status: Completed
Nature: Research.
Students involved: Undergraduate Courses: (2) / Academic Master’s: (2)
Members: João de Jesus Viana Pinheiro - Member / Ruy Gastaldoni Jaeger - Member / Fabrício Mesquita Tuji - Member / Sérgio Melo Alves Júnior - Coordinator / Maria Sueli da Silva Kataoka - Member / José Antônio Picanço Diniz Junior - Member / Aline Semblano Dias Carreira - Member / Alann Thaffarell Portilho de Souza - Member / Marina Rolo Pinheiro da Rosa - Member / Nayara Picanço Kataoka - Member / Nelson Antonio Bailão Ribeiro - Member.
Funding: National Council for Scientific and Technological Development – Financial Aid.
Number of S, T & A Productions: 1
2010 - 2012
In Vitro Study of the Interrelation between Expression of Growth Factors and Expression of Matrix Metalloproteinases in Pleomorphic Adenoma
Description: Among the neoplasias that affect the salivary glands, the most frequent is the pleomorphic adenoma. Despite its slow growth and benign behavior, there is evidence that the neoplastic and stromal cells of this tumor secrete MMPs, possibly indicating the participation of these enzymes in their process of local invasiveness. However, the expression of MMPs and their regulatory mechanisms in pleomorphic adenoma have been poorly documented. The development of tumor cell lines is a procedure that has been used by several researchers in order to better understand the biological behavior of the most varied neoplasms in vitro, as well as to contribute to the advances in the prognosis of the lesion, diagnosis and therapeutics. The previous establishment and characterization of a cell line derived from human pleomorphic adenoma will allow us to carry out more mechanistic studies using gene silencing (siRNA). The methods that will be used will allow correlating the expression of growth factors with the expression of MMPs. According to the established correlation, it is expected to elucidate the way in which growth factors can modulate the expression of MMPs and their participation in local remodeling and invasiveness mechanisms of this neoplasm. Proof of the correlation of these molecules may be of great importance for the treatment of pleomorphic adenoma.
Status: Completed
Nature: Research
Students involved: Undergraduate Courses: (4) / Academic Master’s: (2) / Doctorate: (1)
Members: João de Jesus Viana Pinheiro - Coordinator / Ruy Gastaldoni Jaeger - Member / Sérgio Melo Alves Júnior - Member / Maria Sueli da Silva Kataoka - Member / José Antônio Picanço Diniz Junior - Member / Adriane Sousa de Siqueira - Member / Aline Semblano Dias Carreira - Member / Nayara Picanço Kataoka - Member / Nelson Antonio Bailão Ribeiro - Member / Maria Clara Rodrigues Pinheiro - Member / João Rafael Habib Souza Aquime - Member / Lara Carolina D?Araújo Pinto - Member / Marina Rolo Pinheiro da Rosa - Member.
Funding: National Council for Scientific and Technological Development - Financial Aid.
Number of S, T & A Productions: 1
2009 - 2015
Oral Health in the Island of Marajó: diagnosis of the problems and proposal of the implementation of public policies of dentistry
Description:
The Marajó archipelago, given the size of the social demands, the lack of structural basic services and its environmental characteristics, has high levels of poverty, resulting in low educational level, high infant mortality rates, morbidity due to endemic diseases and consequently, scarce health services, resulting in one of the lowest levels of quality of life in the country. Oral diseases are part of this overwhelming picture, culminating in a process of social exclusion and low human development. The epidemiology of oral diseases in Brazil in recent years shows a more encouraging picture of the knowledge of the diseases and the possibility of planning new services. This encouragement is not the same in all regions of the country and the Northern region still needs knowledge of the epidemiology of diseases and better access to services, since the surveys of oral health conditions in the State of Pará are scarce, making it difficult to plan actions that cover the treatment needs of the population. Most of the epidemiological data available refer to the metropolitan area of Belém. This project proposes to collect epidemiological data and to know the profile of oral health in municipalities located in Marajó Island, in the State of Pará. The sample will consist of 1,171 individuals, distributed according to the size of each selected municipality, observing the age groups or ages indexes of 5 years, 12 years, 15 to 19 years, 35 to 44 years and 64 to 74 years. The oral health conditions related to dental caries and periodontal disease, the most prevalent diseases of the oral cavity, will be raised; use and need of dental prosthesis, aiming at the adequate functionality of the stomatognathic system; and oral neoplasms, which is one of the primary neoplastic sites most incident to proportional cancer mortality. All surveys will be made through the indexes.
Status: Completed
Nature: Research
Students involved: Undergraduate Courses: (2) / Academic Master’s: (2)
Members: João de Jesus Viana Pinheiro - Member / Maria das Graças Rodrigues Pinheiro - Member / Maria Sueli da Silva Kataoka - Member / Regina Fátima Feio Barroso - Coordinator / Sergio de Melo Alves Jr. - Member / Marizeli Viana de Aragão Araújo - Member / Alda França Costa - Member / Ceci Baker de Melo - Member.
Funding: Paraense Amazon Foundation for Research Support - Financial Aid.
2009 - 2013
Immunohistochemical Study of Tumor Signaling Pathways in Ameloblastoma. Immunoexpression and Correlation of Akt, NF-κB, β-Catenin, Cyclin-D1 and Cox-2 Proteins
Description:
Since ameloblastoma is a benign tumor, but with aggressive and recurrent behavior and of great incidence, many researches have been carried out in order to better understand its pathogenesis, progression and bone invasion. Many of these researches aim to identify biological markers, such as metalloproteinases and laminins, in order to elucidate the tumor evolution and to unveil less aggressive therapeutic strategies than the current ones. Because, due to the high rate of recurrence, the main treatment is the hemisection of the affected area, greatly impairing the patient's social life. The AKT, β-Catenin, Cyclin-D1, COX-2, NF-қB proteins have been proposed as biological markers of great relevance in neoplastic progression. Studies have also raised the interaction of these proteins as a signaling pathway for other head and neck neoplasms, such as squamous cell carcinoma. Thus, it becomes valid to evaluate the expression of the aforementioned proteins in order to better understand the pathogenesis of the tumor and to raise new therapeutic perspectives for it.
Status: Completed
Nature: Research.
Students involved: Undergraduate Courses: (1) / Academic Master’s: (1).
Members: João de Jesus Viana Pinheiro - Coordinator / Vanessa M. Freitas - Member / Ruy Gastaldoni Jaeger - Member / Sérgio Melo Alves Júnior - Member / Maria Sueli da Silva Kataoka - Member / Rodolpho Lobão Cecim - Member.
Number of counselings: 2
2009 - 2011
Dento-Skeletal Manifestations of Gaucher Disease
Description:
Research Project that covers all 14 patients with Gaucher Disease in the state of Pará, diagnosed and treated at the Center for Hematology and Haemotherapy of Pará - HEMOPA. After consent, patients undergo physical examination and imaging including panoramic radiographs, periapical and cone beam computed tomography for the purpose of diagnosis of bone changes in the face of such patients. Considering that this disease of the lysosomal deposit presents as main characteristics visceromegalias, pancytopenals and bone alterations.
Status: Completed
Nature: Research.
Students involved: Undergraduate Courses: (0) / Specialization: (0) / Academic Master’s: (1) / Professional Master’s: (0) / Doctorate: (0)
Members: João de Jesus Viana Pinheiro - Coordinator / Rafaela M. Nobre - Member.
Funding: Genzyme do Brasil - Financial Aid
Number of S, T & A Productions: 1
Number of counselings: 1
2009 - 2010
Different Isoforms of Laminin and its Bioactive Peptides Regulating Migration and Invasion of Cell Lines Derived from Carcinomas and Adenocarcinomas
Description: Malignant neoplasias affecting the head and neck region have been studied by many authors, given their frequency, morbidity and high mortality rates that make them a public health problem. Metastasis, the major cause of mortality, is an extremely analyzed phenomenon, but it still needs investigations to clarify its mechanisms. Invasion and cell migration are the main mechanisms that a neoplastic cell uses to cause metastases. These phenomena cause different molecules involved in cell migration to be targets of therapeutic strategies in the fight against a wide range of diseases. Therefore, knowledge of the molecular interactions that regulate the migration of a given cell type is crucial, not only in terms of biological knowledge, but especially in its medical application. On the other hand, the activity of bioactive peptides in different diseases has been analyzed in the literature, not only to elucidate its possible role in the development of pathologies, but also as a strategy to discover potential therapeutic drugs. This project follows this line of investigation, evaluating potential stimulators and inhibitors of tumor migration and / or invasion of neoplasms. In view of the experience accumulated in the area of in vitro study of the effects of laminin in tumor biology, we intend to extend our results by correlating new data to be obtained in vitro with in vivo studies of laminin in human carcinoma and adenocarcinomas. We will start by studying in vivo by immunohistochemistry the expression of different laminin chains in human tumors. Since laminin is cleaved by extracellular matrix metalloproteinases (MMPs), we will also analyze the distribution of these proteases in carcinomas and adenocarcinomas. We will then complement this in vivo study through biochemical analysis of laminin and MMPs in these tumors.
Status: Completed
Nature: Research
Students involved: Undergraduate Courses: (1) / Academic Master’s: (2) / Doctorate: (1)
Members: João de Jesus Viana Pinheiro - Member / Ruy Gastaldoni Jaeger – Coordinator
Funding: National Council for Scientific and Technological Development - Financial Aid
Number of S, T & A Productions: 4
2008 - 2015
Epidemiological Survey of Oral Health in the Municipalities of Curuçá, Maracanã, Salinópolis and São João De Pirabas. Salgado Region of the State of Pará
Description:
This project is a consortium between the Dentistry Graduate Program of UFPA and the Dentistry Graduate Program of UFMG, aiming at the interaction of epidemiological surveys of caries and oral cancer, the use of phytotherapeutic drugs for popular use and its anti-neoplastic action in Cell Cuture, bringing to this study an epidemiological and biotechnological profile.
Status: Completed
Nature: Research
Students involved: Undergraduate Courses: (2) / Academic Master’s: (1)
Members: João de Jesus Viana Pinheiro - Coordinator / Maria das Graças Rodrigues Pinheiro - Member / Maria Sueli da Silva Kataoka - Member / José Antônio Picanço Diniz Junior - Member / Ricardo Alves de Mesquita - Member / Regina Fátima Feio Barroso - Member / Erick Nelo Pedreira - Member / Sergio de Melo Alves Jr. - Member / Maria Cássia Ferreira Aguiar - Member / Marizeli Viana de Aragão Araújo - Member.
Funding: Paraense Amazon Foundation for Research Support – Financial Aid
Number of S, T & A Productions: 2
2008 - 2012
Isolation and Characterization of Cells Derived from Central Nervous System Tumors
Description:
In this project, cell cultures derived from CNS tumors will be used, an important model that has been used by many researchers in the study of the behavior of tumor cells, understanding the transformation processes by which these cells pass, their morphological, biochemical and genetic alterations, until the identification of drugs that could interfere or control the mechanisms of tumor genesis. The use of this methodology has helped to understand several mechanisms developed by neoplastic cells from the interactions with the extracellular matrix, invasiveness and chromosomal alterations to the most current genetic therapies. Although several companies currently produce and commercialize cell lines from several tumors, the acquisition of these lines is highly expensive and some are not even available, such as cell lines derived from benign, untransformed tumors. Unlike malignant cell lines, which are usually immortal, in the benign tumor cell lines the cells age, limiting the experimental studies. This work aims at the establishment and characterization of tumor cell lines, benign and malignant, which will allow the production of cells in a significant quantity for cytogenetic analyzes and the development of studies of interactions with pathogens. These procedures constitute the first step for future in vitro studies of the effects of drugs and / or phytotherapics on the control of some CNS tumors, from tumor cells isolated from patients from the North.
Status: Completed
Nature: Research
Students involved: Undergraduate Courses: (2) / Academic Master’s: (1)
Members: João de Jesus Viana Pinheiro - Coordinator / Maria Sueli da Silva Kataoka - Member / José Antônio Picanço Diniz Junior - Member / Juarez Antônio Simões Quaresma – Member
Funding: Paraense Amazon Foundation for Research Support - Financial Aid
Number of S, T & A Productions: 25
2008 - 2011
Evaluation of MMP-2 and MMP-9, TIMP-1 and TIMP-2, Growth Factors EGF and TGFα, its Receptor, EGFr and Protein ERK and its Active Form pERK in Odontogenic Keratocystic Tumor
Description:
This project aims to evaluate by immunohistochemistry the expression of proteins related to local invasiveness and proliferative activity that may influence the biological behavior of keratocystic odontogenic tumor. Tissue samples from the oral pathology laboratory of the University of Pará College of Dentistry will be used, in which immunohistochemistry will be performed using the immunoperoxidase technique with antibodies to MMP-2 and MMP-9, TIMP-1 and TIMP-2, EGFr, TGFα, EGF, ERK 1/2 and pERK 1/2. This project is based on the theory of MMPs as the focal point of a series of events that could culminate in increased TIMP-regulated protein synthesis, cell proliferation and invasion. The MMPs present in the TOQ would promote localized proteolysis of the ECM, facilitating the release of a series of factors with biological activity, among them, growth factors. These growth factors upon reaching specific receptors on the cell membrane (EGFr), would activate a cascade of cellular signaling proteins (ERK). This chain of events would lead to an increase in proliferative activity inducing mitoses, as well as an increase in the protein synthesis of more MMPs in an autocrine feeding process.
Status: Completed
Nature: Research
Students involved: Undergraduate Courses: (0) / Specialization: (0) / Academic Master’s: (2) / Professional Master’s: (0) / Doctorate: (0)
Members: João de Jesus Viana Pinheiro - Coordinator / R G Jaeger - Member / Maria Sueli da Silva Kataoka - Member / Regina Fátima Feio Barroso - Member / Sergio de Melo Alves Jr. - Member / André Luis Ribeiro Ribeiro - Member / Rafaela Moutinho Nobre – Member
Number of S, T & A Productions: 1
Number of counselings: 1
2007 - 2009
In vivo and in vitro study of the Interrelation between Proliferative Activity and Secretion of Proteases in Pleomorphic Adenoma
Description:
This project, unprecedented in the North Region of the country, aims to implement methodologies that were incorporated by the Coordinator of the project during his doctorate at the Faculty of Dentistry of USP (Oral Pathology), a course graded seven by CAPES. The present study will enable the implantation of these technologies in the undergraduate and graduate courses in Dentistry of the Federal University of Pará, resulting, therefore, in compliance with what is recommended in the recent initiatives of the regional research agencies in Brazil, regarding the greater integration of the institutions of the national regions, in terms of Research and graduate studies, given the need to consolidate research in emerging institutions, which depends on the qualification of its professors and technicians. It is expected to correlate the expression of growth factors with the expression of the MMPs and TIMPS studied in the pleomorphic adenoma (AP). According to the established correlation, we will have taken an important step in elucidating the way in which growth factors can modulate the release of MMPs, their possible interactions with TIMPS and the participation of these growth factors with the remodeling of the AP.
Status: Completed
Nature: Research
Students involved: Undergraduate Courses: (1) / Academic Master’s: (1)
Members: João de Jesus Viana Pinheiro - Coordinator / Ruy Gastaldoni Jaeger - Member / Maria Sueli da Silva Kataoka - Member / José Antônio Picanço Diniz Junior - Member / Edmar Tavares da Costa - Member / Marina Rolo Pinheiro da Rosa - Member / Aline Semblano Dias Carreira – Member
Funding: Paraense Amazon Foundation for Research Support - Financial Aid
Number of S, T & A Productions: 2
Number of counselings: 2
379.918.172-53 - Jose Thiers Carneiro Junior
2016 - Current
Tomographic Evaluation of Dental and Skeletal Alterations in the Rapid Expansion of the Surgically Assisted Maxilla. Comparison between Transpalatal Bone Dystoric and Dental Supported Disjunction
Description:
Theoretical basis and guiding questions: the indications for surgically assisted rapid expansion of the maxilla are skeletal maturity, transverse maxillary hypoplasia, either uni or bilateral and narrow buccal corridor. The treatment is a combination of surgical procedure and orthodontic treatment provided by means of osteogenic distraction, providing space in the dental arch for the alignment of the dentition. The procedure causes a substantial enlargement of the maxillary base and the palatine vault. Traditionally, an orthodontic appliance called the Hyrax type expander is placed preoperatively to expand the maxilla through dental anchorage, however, such anchoring may cause several undesirable side effects, including damage to the dentition, possible loss of anchorage, compression of the periodontium, root resorption, cortical fenestration. To avoid these complications, several bone-anchored devices (transpalatal distractors) have been developed. These are installed directly on the palatal bone bi-laterally during the surgery. It is claimed that they avoid several problems encountered with hyrax. The advantage of bone-supported devices is that the forces are applied at the desired level of position, avoiding dental vestibularization, in addition to maintaining a minimal segmental inflection. Formulation of the objectives: To evaluate the difference between the two groups, considering the amount of recurrence, the amount of displacement of the maxilla and the bone quality formed. Hypothesis: The transpalatina maxillary distraction presents better results as the decrease of the undesirable effects caused by Hyrax. Methodology: A prospective randomized clinical study comparing the difference between the groups regarding stability and bone quality through linear measurements and bone density obtained on pre, immediate postoperative, post-surgical, 6-month, and 12-month post-surgical. The surgery will be the same for all cases, on general anesthesia, with leftotic corticotomies with section of nasal lateral wall, pterygoid disjunction and osteotomy between central incisors. Sampling: 20 patients treated with Hyrax and 20 patients treated with transpalatal distractor after surgically assisted maxillary expansion surgery operated by a single surgeon at T0, T1, T2 and T3 times before and after surgery. This sample size is effective to demonstrate a significant difference between groups at 5% error and statistical test power at 90%. Exclusion criteria are patients younger than 16 years, syndromic patients (including cleft palate), irradiated or mentally incapacitated patients. Expected procedures: collect and analyze high-precision quantitative data from patients' tomographic measurements, obtained digitally through softwares. Variables to be analyzed: quantity and quality of dental expansion of molars and maxillary premolars as well as bone level of palatal expansion and nasal base in the two distinct methods of expansion, in the preoperative, immediate postoperative, postoperative period of 6 months and postoperative of 12 months. Predicted statistical method: completely randomized design, with 2 distinct clinical treatments containing 20 samples each. Comparison of the means through the multivariate statistical test of anova with post-roc of t. Students, seeking differences between groups. Expected results: we did not find statistically significant differences between the two groups, with distinct palatine expansion at the canine, premolar and molar levels. Significant increase in bone distance maintained, with the maxilla moving slightly downward. Less vestibular bone fenestration and lower vestibularization in the transpalatal distractor.
Status: In progress
Nature: Research
Students involved: Academic Master’s: (1)
Members: José Thiers Carneiro Junior - Coordinator / Douglas Voss – Member
2016 - Current
Analysis of Finite Elements on Implantable Expansion Devices
Description:
Introduction: Transverse maxillary deficiency is characterized by atresic maxillae, oval palate, crowding and dental rotation, as well as posterior crossbite (uni or bilateral) (Nary Filho, Gonçales and Ribeiro Jr., 2004). The effects of surgically assisted expansion are not only seen in the upper dental arch; its effects on the nasal cavity have been known since 1961 (Haas, 1961). Starnbach et al. (1966) also reported dental alterations following the expansion procedure, since the expander apparatus causes compression of the periodontal ligament, causing the supporting dental element to move towards the expansive movement. The treatment of patients with transverse maxillary deficiency aims to increase transverse dimensions, and in patients with skeletal maturity, surgically assisted maxillary expansion (EMCA) has shown good results (Lanigan and Mintz, 2002; Ribeiro Jr, 2006). With the technological advancement, finite element analysis has been used more frequently to verify tensions (traction and compression) distributed along a studied object. The finite element method (MEF) was created for applications in aerospace engineering, but over the years it has been adapted for use in biomodels, allowing the study of parts of the human body under different hypotheses.
The method fragments the study model into small parts (finite elements) that join together to form a mesh. Mathematical equations applied to these interconnected elements simulating the hypotheses raised make the answer to be interpreted by the whole (study model) (Lotti, 2006). In 2012, Assis demonstrated through MEF that variations of Le Fort I osteotomies influence the tensile and compression tensions that dissipate through the maxilla. The tensile tension was concentrated in the meeting of the alveolar ridge with the horizontal blade of the palate leading to the posterior region with tension dissipation on the pterygoid process of the sphenoid bone. These tensions were significantly neutralized when the osteotomy included separation of the maxilla from the pterygoid process. Some undesirable effects related to the EMCA procedure are related to the type of expander used, such as dental changes after the expansion procedure, since the dento-supported expander apparatus causes compression of the periodontal ligament, causing the supporting dental element to move in the direction of the expansive movement, in addition to radicular and alveolar resorption (Garib, 2006). To avoid undesirable effects on the teeth that support the expanders, devices supported on screws and palatal implants have been created and used (Boyor, 2013). Objective: The objective of this study is to observe the behavior of the tensions on the implants and the maxilla (von Misses strain) to evaluate the critical stresses and behavior of the study bodies. Methodology: the implant-supported device will be modeled in CAD together with the jaws, generating a mesh of finite elements on the model. An opening of 1 mm will be imposed on the expander screw (corresponding to the initial opening of the procedure) and to verify how the tensions on the bodies in this condition behave. Expected results: the prediction in this study will be the accumulation of tensions at the ends of the horizontal rods of the expander apparatus, in the region of union with the implants that support it, dissipating these tensions to the posterior region of the jaw, thus freeing the teeth from this condition.
Status: In progress
Nature: Research
Students involved: Academic Master’s: (1)
Members: José Thiers Carneiro Junior - Coordinator / Fernando Jordão de Souza Júnior - Member / Diogo Souza Ferreira Rubim de Assis - Member / Newton Guerreiro da Silva Junior – Member
2015 - Current
Comparison of Stability of Maxillary Advancement in Le Fort I Osteotomy through Cone Bean Tomography between Rigid Internal Fixation with Four Mini Plates Fixation with Two Mini Plates in Canine Pillars and Steel Wire in Two Zygomatic Pillars
Description: Le Fort I type osteotomy, alone or in combination with mandibular osteotomy, is commonly used for the correction of dentofacial deformities. The literature presents several studies documenting the stability of these procedures obtained with mini-plates and screws (Baker DL, 1992, Kwon, TG, 2000) or with mini plates and steel wire (Quejada, JG 1987) while others compared the use of both. (Skoczylas, 1988, Ish LC, 1987). Currently, the tendency of most surgeons is to use stable internal fixation with 4 mini plates and screws as a method of osteosynthesis for the Le Fort I osteotomies. However, this type of fixation is not always successful because its adaptation and its contour may displace the newly positioned jaw and produce an undesirable effect on dental occlusion. There are no scientific data or rules that determine the exact number of mini plates required for proper fixation of the maxilla and a consequent prevention of postoperative skeletal recurrence. This issue may have important implications, considering the addition of material costs and surgical time generated by the use of a greater amount of osteosynthesis material. Objective of the study: the objective of this study is to compare the osteosynthesis obtained with the fixation of the maxilla by the method of 4 mini plates and screws with the osteosynthesis obtained in the method of two mini plates with steel wire.
Using current surgical techniques and cutting-edge diagnostic technology with the use of cone beam tomography, to add new scientific data to this discussion. Hypothesis: it is possible to reduce the amount of the material for osteosynthesis and the time of surgery, maintaining adequate stability of the maxilla. Methodology: Type of study: prospective study to evaluate the postoperative stability of maxillary advancement with Le Fort I osteotomy. Ophir Loiola Hospital Sampling: 20 patients treated by a single surgeon. Over a period of 36 months. 10 patients will be treated with four mini plates, two on the canine abutments and two on the zygomatic abutments, another 10 will be treated with two mini plates on the canine abutments and steel wire on the zygomatic abutments. Predicted procedures: Patients treated by maxillary advancement by Le Fort I osteotomy will be evaluated by preoperative lateral cone bean tomography and postoperative tomography of 15 days and 6 months, measuring the skeletal changes occurring both in the sense of movement as in the opposite direction through cephalometric measurements applied on lateral tomography. Variables to be analyzed: Skeletal changes occurring both in the direction of movement and in the opposite direction through cephalometric measurements applied on the lateral tomography pre- and postoperative. Predicted statistical method: a completely randomized design with 2 treatments and 10 replicates. Comparison of means by t test. Correlation coefficients will be applied by multivariate analysis. Statistical design. Expected results: the data obtained will allow a comparative analysis of the skeletal changes in maxilla treated by Le Fort I osteotomy using fixation by 4 mini-plates and screws or using 2 mini plates and steel wire.
Status: In progress
Nature: Research
Students involved: Specialization: (3) / Academic Master’s: (1)
Members: José Thiers Carneiro Junior - Coordinator / Ana Karla da Silva Tabosa - Member / Bruno Thiago Cruz e Silva - Member / Francisco de Souza Neves - Member / Douglas Voss – Member
2015 - Current
Evaluation of bone repair in patients with cleft lip and cleft palate using rhBMP-2
Description:
The alveolar graft will allow the fissured patient a continuity of the maxilla that previously did not exist. Secondary bone graft is preferred today as a protocol in the treatment of patients with cleft lip and it is also the most indicated by orthodontists because it offers children a superior aligned intact dental arch and without need for prostheses in the cleft area. The tertiary bone graft is performed after the end of the corrective orthodontic treatment in the permanent denture (SILVA FILHO et al., 2000; GARIB et al., 2011). The careful attention to the health of the patient of this condition promotes an improvement in their quality of life since they present difficulties related to speech, swallowing, social life as well as challenges directed to the correction of facial esthetics. At the moment of the secondary or tertiary bone graft for cleft closure, autogenous bone is used as the standard and donor areas include the iliac, rib, tibia and skullcap. These areas bring with them an increase in surgical morbidity for longer intraoperative as well as possible complications such as bleeding, hematoma, chronic pain, pneumotorax, undue fractures.
The performance of the dental surgeon is very intense, as it begins whenever possible shortly after birth and ends when the dental occlusion is normalized according to the individual characteristics of each case. Today we have a new choice of material for rehabilitation of patients with cleft lip palates, the rh Bone Morphogenetic Protein-2 (rhBMP-2) which is a growth factor that induces a new bone formation. RhBMP-2 is of great interest to surgeons because it allows for reduced time and surgical morbidity by eliminating the need for a donor bed. Objectives: To use rhBMP-2 as a surrogate for iliac crest graft in patients with cleft lip palates by collecting their results and verifying whether it can be justified as a good treatment option for the gold standard. Materials and methods. The data analyzed in this study will be collected from patients at the Ophir Loyola Hospital, from the department of buco-maxillofacial surgery, from May 2015 to May 2017, totaling 20 patients. Individuals will be selected between men and women with cleft palatal complete with variable age, already submitted to surgery of queiloplasty and palatoplasty, with orthodontic preparation that present the segments of the upper arch aligned and level with each other, the patients will be operated by a single surgeon. Individuals will be divided into two groups, control and test. The former will receive iliac crest graft and the test will receive rhBMP-2. The tomographic analyzes of the grafted area will be performed by Cone Bean tomography with sagittal sections of the cleft area. CT scans will be classified into: preoperative (T0), 7-day postoperative (T1), 1-month postoperative (T2) and 6-month postoperative (T3).
Status: In progress
Nature: Research
Students involved: Specialization: (2) / Academic Master’s: (1)
Members: José Thiers Carneiro Junior - Coordinator / Bruno Thiago Cruz e Silva - Member / Francisco de Souza Neves - Member / Ana Karla da Silva Tabosa – Member
2008 - 2010
Risk Assessment of Inferior Alveolar Nerve Injury in Molar Third-Party Exodontia
Status: Completed
Nature: Research
Students involved: Doctorate: (1)
Members: José Thiers Carneiro Junior - Coordinator / Rommel Mario Rodriguez Burbano – Member
2002 - 2005
Ultrastructure and Mesoscopy of Elements of Digestive Tract
Status: Completed
Nature: Research
Members: José Thiers Carneiro Junior - Coordinator
2611242 - Juliana Melo da Silva (743.359.522-87)
2017 - Current
Confocal Microscopy Evaluation of Intracanal Injection of Calcium Hydroxide by Different Irrigants and Ultrasonic Activation
Status: In progress
Nature: Research
Students involved: Academic Master’s: (2)
Members: Juliana Melo da Silva Brandão - Coordinator / Joao Nogueira Leal Silva, Emmanuel - Member / Roberta Fonseca de Castro - Member / Luiz Carlos de Lima Dias Junior - Member / Bruna Araújo Smith – Member
2017 - Current
Effect of Irrigation with Sodium Hypochlorite and Surfactants on the Adaptation of an Epoxy Resin Based Cement to Root Dentine
Status: In progress
Nature: Research.
Students involved: Academic Master’s: (2)
Members: Juliana Melo da Silva Brandão - Coordinator / Emmanuel João Nogueira Leal da Silva - Member / Roberta Fonseca de Castro - Member / Luiz Carlos de Lima Dias Junior – Member
2016 - Current
Epidemiology
Description: It studies the oral health conditions of typical populations of the Amazon, in the urban context, riverside, indigenous and quilombolas, through studies based on the health-disease process, oral health indicators and health systems. It is an objective, to monitor the effects of changes resulting from the process of acculturation of traditional populations in the manifestation of oral diseases and changes in dental occlusion.
Status: In progress
Nature: Research
Students involved: Undergraduate Courses: (3) / Academic Master’s: (5)
Members: Juliana Melo da Silva Brandão - Member / Brandão, Gustavo Antônio Martins - Member / David Normando - Coordinator / Helder Pinheiro – Member
2016 - Current
Confocal Microscopy and its Applicability to Endodontics
Description:
This project aims to use the confocal laser scanning microscope as an in vitro laboratory research method. Evaluating the filling quality of the material and sealant in the root canals and dentinal tubules, varying the type of sealant material used, chemical auxiliary substances used, different instrumentation and cleaning protocols to remove debris and smear layer. This method of analysis does not require special processing of the specimens, reducing the potential of technical artifacts. Confocal laser scanning microscopy (CLSM) is performed using fluorescent dyes and allows the analysis of materials with different composition.
Status: In progress
Nature: Research
Students involved: Undergraduate Courses: (2) / Academic Master’s: (2)
Members: Juliana Melo da Silva Brandão - Coordinator / Emmanuel João Nogueira Leal da Silva - Member / Roberta Fonseca de Castro - Member / Luiz Carlos de Lima Dias Junior - Member / Bruna Araújo Smith - Member / Daniela Feitosa Saavedra - Member / Francisco Montagner – Member
2015 - 2016
Correlation of Instrumentation with NI-TI Rotary Files in Amount of Extrusion of Dentinary Debris after Foraminal Amplification
Description:
During the operative procedure, the use of systems and solutions capable of completely sanitizing the root canals without developing undesirable inflammatory reactions are essential for successful endodontic treatment. The objective of this work is to evaluate the amount of extruded dentinal debris during the preparation of root canals. Using different reciprocating systems and instrumenting them at zero and 1mm below the working length. Contributing positively with the works developed today. The objective of the present study was to analyze the amount of dentinal debris extruded apically after instrumentation at zero and 1mm below the working length (CT – Portuguese acronym), using three different rotary systems that recommend a single file to prepare the root canal: R40 Reciproc® System (VDW, Munich, Germany), Large 40 WaveOne® (Dentsply Maillefer, Ballaigues, Switzerland) and SF 40.06 ProDesing Logic (Easy, Belo Horizonte, Brazil).
Status: Completed
Nature: Research
Students involved: Undergraduate Courses: (1) / Academic Master’s: (1)
Members: Juliana Melo da Silva Brandão - Member / Juliana Melo da Silva - Coordinator / Juliana do Socorro Soares Melo - Member / Roberta Fonseca de Castro - Member.
Number of counselings: 1
2015 - Current
Penetrability of Endodontic Cement after Different Retreatment Protocols: a Confocal Study
Description:
Endodontic retreatment is the first-choice option for cases of failure. During the removal phase of the material, the ideal is to obtain a dentin wall free of remnants, but no available technique provides complete removal of the sealing material and this may interfere with the quality of the post-retreatment filling. The objective of this study is to evaluate the penetrability of the post-retreatment endodontic cement, varying the solvent use and the type of irrigant used. Ten mesial roots of lower molars will be selected. To standardize the root length, the crowns of the teeth will be removed at the level of the cementoenamel junction, establishing a length of 12mm for each mesial root of the lower molars. The biomechanical preparation of the specimens will be performed 1mm from the apical foramen using rotating files from the Protaper Universal system to the F3 file. Firstly, the specimens will be randomized into three groups according to the irrigant used: one group will be irrigated with 3 ml sodium hypochlorite 1%; another with 0.5 ml of chlorhexidine gel 2% and 3 ml of saline solution and; the other will be irrigated with 3ml of saline at each exchange of file.
The final lavage will be done with 3 ml of the main irrigant, 5 ml of saline followed by 3 minutes of 17% EDTA and then 5 ml of saline solution again. For the obturation, the main gutta-percha cones of the ProTaper system and AH Plus cement will be used. For observation in confocal microscopy, the dye Rhodamine B, with a concentration of 0.1%, will be added to the cement. The samples will then be stored for 7 days at 100% humidity and 37 ° C, so that after this period they can be submitted to retreatment. At this moment, the samples will be randomized and divided into subgroups: a- Retreatment with ProTaper Retreatment system; b- Retreatment with ProTaper system Retreatment + Solvent Eucaliptol; c- Retreatment will not be performed and will serve as control. Irrigation and obturation will be performed similar to the treatment phase. The dye used for this second filling will be Fluorescein with 0.1% concentration to the AH Plus cement. The samples will be sectioned on 2mm discs at 2, 4 and 6mm at the apex, then polished and taken to confocal laser scanning (MCVL) microscopy. The images obtained will be taken to an image editing program to analyze the results. The data will be collected, tabulated and analyzed for normality for subsequent selection of the statistical test. The significance level of 5% will be adopted. Keywords: Endodontics; Retreatment; Confocal Microscopy; Root canal obturation.
Status: In progress
Nature: Research
Students involved: Academic Master’s: (1)
Members: Juliana Melo da Silva Brandão - Member / Juliana Melo da Silva - Coordinator / Emmanuel João Nogueira Leal da Silva - Member / Roberta Fonseca de Castro – Member
2015 - Current
Influence of Single File Systems and Retraction Files on Apical Deviation
Description:
Due to the large number of channel deviations and the limitations they can cause during instrumentation and in the later phases, as well as in the disinfection of the channels, this work has the objective of verifying which of the three studied files cause less deviation of the root canal. Since it is essential for the success of the treatment during the instrumentation that the professional maintains to the conicity and original curvature of the canal, the present study has the objective of analyzing radiographically the occurrence of apical deviation in curved channels after instrumentation and after retreatment. To analyze the amount of apical deviation that three systems and single files to prepare the canal cause and compare them, as well as the risk that the need for a reintervention in the root canal causes in relation to the apical deviation.
Status: In progress
Nature: Research
Students involved: Undergraduate Courses: (1) / Academic Master’s: (1)
Members: Juliana Melo da Silva Brandão - Member / Juliana Melo da Silva - Coordinator / Débora Ferreira Monteiro da Silva - Member / Roberta Fonseca de Castro - Member.
Number of counselings: 1
2015 - Current
Effects of Bioactive Agents in the Treatment of Dental Bleaching
Description:
The project aims to analyze the effects of bioactive agents on the surface of the dental enamel before dental bleaching. In vitro analyzes will observe the macro and microscopic morphology of dental tissue before and after bleaching. The project integrates several specialties in four work plans initially proposed: 1) Does fluorine in high concentrations prevent changes in bleached enamel? - in vitro evaluation of the effect of fluoride in high concentration (5000 pmm) on the surface of human dental enamel after bleaching with 35% hydrogen peroxide by Vickers microhardness, roughness and colorimetry. 2) The effect of strontium chloride and potassium nitrate associated with the home bleaching treatment on bovine enamel. - In vitro study of the effect of 10% strontium chloride and 2% potassium nitrate of 35% hydrogen peroxide bleached bovine enamel by Knoop microhardness, surface roughness and triistimulus colorimetry. 4) In vitro effect of arginine in different concentrations associated with calcium carbonate after dental bleaching: an in vitro study of the effect of dentifrices containing different concentrations of arginine (8% and 12%) associated with 40% calcium carbonate applied on the enamel bleached with hydrogen peroxide in high concentration on the hardness, roughness, morphology and color of the enamel.
Status: In progress; Nature: Research.
Students involved: Undergraduate Courses: (3) / Academic Master’s: (5).
Members: Juliana Melo da Silva Brandão - Member / Juliana Melo da Silva - Member / Oscar Faciola Pessoa - Member / Patricia de Almeida Rodrigues - Member / Cecy Martins Silva - Coordinator / Mário Honorato Silva e Souza Júnior - Member / Sandr Cordeiro Loreto - Member / Roberta Souza D'Almeida Couto - Member.
2015 - Current
Influence of Etidronate on Microhardness and Dentin Roughness
Description: Many chemical substances can be used during the biomechanical preparation of the root canal, in order to completely remove Smear Layer. Among these chelating substances, we highlight the ethylenediaminetetraacetic acid (EDTA) and Etidronate (HEPB). This work aims to analyze the influence of the use of HEPB on the roughness and microhardness of the root dentin through the analysis of thirty half-roots that will be randomly divided into three groups. Group 1 will be immersed in 40 ml of 18% HEPB solution mixed with 2.5% NaOCl. Group 2 will be placed in a solution of 40 ml of 2.5% NaOCl for 30 minutes, simulating the instrumentation, and then be submerged in a solution of 9% HEPB for five minutes simulating final washing. After this, the G2 will be immersed again in a 40ml solution of 2.5% NaOCl for fifteen seconds. Group 3 will be immersed in a solution of 40 ml of 2.5% NaOCl for 30 minutes, simulating the instrumentation process. After this procedure, the solution will be immersed in 40 ml of solution containing EDTA 17% for three minutes. In the last step, the sample will be immersed in 40 ml of solution containing 2.5% NaOCl for 15 seconds. From these procedures, comparisons will be made between the groups using Student t-test (p <0.05) and (ANOVA) (p <0.05) for microhardness analysis. In the case of roughness, the tests used will be Wilcoxon (<0.05) and Friedman test (p <0.05).
Status: In progress;
Nature: Research.
Students involved: Undergraduate Courses: (2) / Academic Master’s: (3).
Members: Juliana Melo da Silva Brandão - Member / Oscar Faciola Pessoa - Coordinator / Patricia de Almeida Rodrigues – Member
2014 - 2015
Evaluation of Apical foramen morphology after use of the Easy Prodesign Duo Hybrid System
Description:
The objective of this research was to evaluate the influence of apical patency performed with Ni-Ti rotary files in the foraminal anatomy. Methods: 30 upper molars of extracted human teeth were selected. The distal vestibular roots (with a standardized length of 12 mm) were used to analyze the foraminal anatomy in a scanning electron microscope. Samples were randomly divided into: Group 1 (n = 15) Rotation lane 25.01 (Easy) and; Group 2 (n = 15) Path File 0.19 (Dentsply). The instrumentation technique was performed with EasyEndo SI® electric motor with torque and speed according to the manufacturer's instructions. As auxiliary chemical in all groups, 2% Chlorhexidine gel associated with saline solution was used as the irrigating solution. Final irrigation was performed with 17% EDTA. For the microscopic analysis, the specific parameters of 3 kV and 50x magnification were used. Two images were taken from each sample, the first one before instrumentation, the second after instrumentation at zero of the localizers. Always with the same orientation, in order to be evaluated and photomicrographs in the same position. Keywords: Endodontics; apical foramen; apical enlargement.
Status: Completed
Nature: Research
Students involved: Undergraduate Courses: (1) / Academic Master’s: (1)
Members: Juliana Melo da Silva Brandão - Member / Juliana Melo da Silva - Coordinator / Débora Ferreira Monteiro da Silva - Member / Roberta Fonseca de Castro – Member
Number of counselings: 1
2014 - 2015
Study of the Internal Anatomy of the Root Canal in Inferior Incisors by the Diaphanization Technique
Description:
No study in the literature reports the prevalence of root canal branches in samples of lower incisors in the North of Brazil. Knowledge of the usual endodontic morphology and its possible anatomical variations is important. The objective of this study was to evaluate the prevalence of canals in lower incisors in the North of Brazil, through the decalcification and diaphanization method. In addition, external measurements were taken and the direction of the apical foramen was observed. 100 teeth were used, where it was observed that 22.37% presented two channels, type 3 being more prevalent and type 5 being the lowest incidence. The mean length of the teeth was 13.7 mm and 76.31% presented the apical foramen coinciding with the long axis of the tooth. Prior knowledge of such changes should be weighed before endodontic therapy. Keywords: Root Canal; Incisive Mandibular; Morphology.
Status: Completed
Nature: Research
Students involved: Undergraduate Courses: (2)
Members: Juliana Melo da Silva Brandão - Member / Juliana Melo da Silva - Coordinator / Brenna M L Nogueira - Member / Juliana do Socorro Soares Melo - Member
Number of counselings: 1
2013 - Current
Pre-Clinical Restorative Dentistry I: development of a practical manual as a teaching tool
Description:
Preclinical activities consist of an important stage of teaching in dentistry, since they are the first contact of students to activities that simulate clinical practice, being based on theoretical-practical activities. In this context, monitoring is a major ally in the teaching-learning process, presenting itself as an instrument for the improvement of undergraduate education, through the development of pedagogical practices and experiences aimed at strengthening the articulation between theory and practice, promoting mutual cooperation between teacher and student (NATÁRIO, 2007). The monitor, because he has already experienced the student situation in this same discipline, is able to capture the possible difficulties of the content, as well as being more sensitive to the student's problems. In addition, the presence of a great volume of practical activities in the module of the discipline points out the possibility of making a practical manual involving the techniques and practices of the discipline, thus being an auxiliary tool in the teaching-learning process. The student / monitor is often a strong facilitator of learning, favoring a better understanding between teachers and students and for bringing to the discussion the learning process from the student point of view. We have previous experiences with students / monitors / volunteers and the results demonstrate that the return is positive for all involved in this academic process. We are convinced that the creation of this Monitoring Program will strengthen undergraduate education and bring progressive benefits.
Status: In progress
Nature: Research.
Members: Juliana Melo da Silva Brandão - Member / Juliana Melo da Silva - Coordinator / Maria Sueli da Silva Kataoka - Member.
2013 - Current
Pulpal Revascularization in Teeth with Incomplete Rhizogenesis: prospective clinical study
Description:
The objective of this prospective clinical study is to evaluate a treatment protocol in teeth with incomplete rhizogenesis through pulp tissue revascularization procedures. After approval by the Ethics Committee in Research in Human Beings of the Institute of Health Sciences of the Federal University of Pará, volunteers will be selected to carry out research. Inclusion criteria will be: patients with immature teeth, with or without signs / symptoms of apical periodontitis, aged 8 to 12 years, and incomplete root formation verified by periapical radiography. Later, the patients will perform computed tomography of the tooth in question, to serve as a comparative analysis and measurement of the thickness of the root walls, as well as measurement of the diameter of the apical foramen. A pulp tissue revascularization protocol will be performed by induction of clot formation into the root canal and insertion of calcium hydroxide and mineral trioxide aggregate (MTA). The tooth will be sealed with composite resin and radiographed for postoperative control obeying the same positioning of the initial periapical radiography. Clinical and radiographic monitoring will be performed every 3 months. Clinically, percussion test, apical palpation, inspection, bleeding at probing, depth of pocket at probing, measurements of the periodontal insertion level, and degree of mobility of the dental element will be performed. The periapical region will be evaluated radiographically; hard blade; height and root thickness. The data obtained will be tabulated and analyzed by means of frequency distribution tables and 95% confidence interval for proportion.
Status: In progress
Nature: Research.
Members: Juliana Melo da Silva Brandão - Member / Juliana Melo da Silva – Coordinator
2012 - 2013
Evaluation of the Fracture Strength of Incomplete Root Formation Teeth Using Different Intracanal Medication Protocols
Description:
The first-choice endodontic treatment for non-vital immature permanent teeth is apexification. Calcium hydroxide is the most used product due to its antimicrobial and osteoinductive properties, but requires long periods to induce formation of calcified apical barrier. It is known that when in prolonged contact with the dental structure, it changes the biomechanical properties of the dentin. The objective of this research was to evaluate the influence of intracanal medication on radicular resistance to fracture in teeth with incomplete root formation from different medication protocols. Thirty bovine teeth were used, which after extraction, the crowns and roots were sectioned. The root canals received biomechanical preparation and irrigation with 1% sodium hypochlorite. The teeth were embrittled with the 703 drill to simulate incomplete rhizogenesis. The roots were included in blocks of acrylic resin and divided into 3 experimental groups - group 1: calcium hydroxide P.A and saline; Group 2: calcium hydroxide P.A gel 2% chlorhexidine digluconate and zinc oxide and Group 3: Control, no intracanal medication received. After 30 days, the specimens were subjected to a compression test at 90° with a speed of 1mm / min until a fracture, the data were subjected to ANOVA statistical analysis (5%) and Tukey test. Mean values of resistance in Kilogram-force were verified, group 1 presented 192,12, group 2 188,57 and group 3 261,12. It is concluded that the use of calcium hydroxide reduces root fracture resistance. Keywords: Apexification; Preparation of root canal; Calcium hydroxide.
Status: Completed
Nature: Research
Students involved: Undergraduate Courses: (2)
Members: Juliana Melo da Silva Brandão - Coordinator / Ana Júlia Lemos - Member / Amarildo Ubirajara da Silva Ferreira – Member
2011 - 2012
Analysis of Coronary Infiltration Faced with Penetration of Nanking in Endodontically Treated Teeth
Description:
To achieve the success of endodontic therapy, it is not enough to have an effective three-dimensional obturation of the root canal system. The acquisition of a good sealing of the coronary cavity using a temporary restorative material to avoid infiltration is also indispensable between the sessions or at the conclusion of the treatment, being this determinant step for the success or failure of the therapy. The aim of this study was to evaluate the efficacy of three temporary restorative materials by means of dye infiltration in order to obtain reliable alternatives to enable the use of materials that achieve the objectives of a good temporary sealing and the prevention of infiltration through the dentinal wall interface / obturator material.
Status: Completed
Nature: Research.
Students involved: Undergraduate Courses: (2)
Members: Juliana Melo da Silva Brandão - Coordinator / Aurilene da Silva Cardoso – Member
2009 - 2011
Influence of Foraminal Enlargement on Apical Anatomy and Sealing Quality After Sealing
Description:
Introduction: The objective of this research was to evaluate the influence of foraminal enlargement and limit of instrumentation on apical anatomy alteration, and sealing capacity after obturation. Methods: Fifty-five palatine roots of maxillary molars of extracted human teeth were selected. Subsequently, the samples were divided into three groups (n = 15) according to the working length, in which group 1 was instrumented 1 mm below the foramen; group 2 was instrumented at the limit of the apical foramen; and group 3 instrumented 1 mm beyond the foramen. In all groups, nickel-titanium rotary instruments were used, using three gage files subsequent to the first instrument that adjusted the working length, and the obturation was performed with AH Plus cement and gutta-percha. Photomicrographs were performed in scanning electron microscopy (SEM), to record the foraminal anatomy before instrumentation, after the use of each instrument, after final instrumentation and after obturation. In addition, in all groups, the coronary infiltration test for Enterococcus faecalis was performed, including positive controls (n = 5) and negative controls (n = 5). Results: the results of this study showed the preserved cementum channel when the instrumentation is performed 1mm short of the apical foramen. Regarding the quality of the foraminal enlargement, there was no statistical difference between the magnification performed at the foramen border and the magnification performed 1mm beyond the foramen. When this enlargement was conducted beyond the apical foramen, larger deviations occurred, but these deviations did not interfere in the quality of sealing or bacterial infiltration. Conclusions: scanning electron microscopy showed a better quality of apical sealing for the groups that foraminal enlargement was performed. However, the experimental model of bacterial infiltration.
Status: Completed
Nature: Research
Students involved: Undergraduate Courses: (1) / Academic Master’s: (1) / Doctorate: (1)
Members: Juliana Melo da Silva Brandão - Coordinator / Zaia, AA – Member
2009 - 2010
Evaluation of Endodontic Treatments performed at the FOP-UNICAMP Undergraduate Clinic: success rate related to the foraminal enlargement technique and use of chlorhexidine gel 2%
Description:
The objective of this study was to evaluate endodontic treatments performed by undergraduate students of the School of Dentistry of Piracicaba - Unicamp - SP, Brazil, using the technique of foraminal enlargement and use of chlorhexidine gel 2% as an auxiliary chemical. The clinical and radiographic criteria used are in accordance with the American Association of Endodontics (Quality Assurance Guidelines, 1987). Clinically percussion and apical palpation tests are performed to observe the presence or absence of symptomatology, inspection of the oral mucosa and inspection of the dental element. Bleeding at the probing, bacterial plaque on the dental surface, depth of probing, measurements of the periodontal insertion level and degree of mobility of the dental element, make up the periodontal clinical indicators. Radiographically the evaluation criteria of the endodontic treatment recorded in the individual record are: (A) normal periapical region with a hard blade surrounding the apex; (B) periapical region with less periapical lesion than before endodontic treatment; (C) periapical region with equal lesion of endodontic treatment; (D) periapical region with greater lesion than before endodontic treatment. All records of adult individuals who have completed endodontic treatment for at least six months will be evaluated by the Researchers. The data obtained will be tabulated and analyzed by means of frequency distribution tables and 95% confidence interval for proportion.
Status: Completed
Nature: Research
Students involved: Undergraduate Courses: (2) / Academic Master’s: (1) / Doctorate: (1)
Members: Juliana Melo da Silva Brandão - Coordinator / Zaia, AA – Member
2006 - 2008
Evaluation of the Cleaning of the Apical Dentinal Surface after the Removal of Different Associations of Calcium Hydroxide
Description:
In order to evaluate the apical dentinal surface, after the removal of different calcium hydroxide slurries (Ca (OH) 2), we selected 68 uniradicular teeth, prepared by a single operator, using the regressive technique. The canals were irrigated with 5ml of 1% sodium hypochlorite (NaOCl) followed by final irrigation with 10 ml of 17% tergene-associated ethylenediaminetetraacetic acid (EDTA-T). The samples were randomly divided into 4 groups (n = 15), according to the type of association of Ca (OH) 2: associated with the chlorhexidine solution 0.2% (Group 1); to propylene glycol (Group 2); to ciprofloxacin, metronidazole and distilled water (Group 3); to ciprofloxacin, metronidazole and propylene glycol (Group 4). Negative control teeth (n = 4) were not filled with Ca (OH) 2, and in the positive control, (n = 4) the Ca (OH) 2 removal was not done. Samples were stored at 37 ° C at 100% relative humidity for 21 days. Removal of the medication was performed with: irrigation with 5 ml of 1% NaOCl; circumferential frame with memory instrument; 5ml 1% NaOCl; patency with K # 10 file; ultrasound with K # 15 file; and 10 ml of 17% EDTA-T. After the application of the Kruskal-Wallis test (α = 5%), it was observed that there was no difference between the groups in relation to the removal of Ca (OH) 2 (p = 0.0951). The chi-square test (α = 5%) indicated the predominance of Ca (OH) 2, obstructing the dentinal tubules, regardless of the vehicle used. After spot chemistry (X-ray dispersion spectrometry - EDS) and Tukey's variance analysis (α = 5%), it was observed that there was a difference in the amount of calcium ions remaining between groups 1 and 4 (p <0.01). Based on the methodology employed, it was concluded that, regardless of the type of vehicle used, the apical dentinal surface also remained covered by Ca (OH)2.
Status: Completed
Nature: Research
Members: Juliana Melo da Silva Brandão - Coordinator / Oscar Faciola Pessoa – Member
2004 - 2005
Deformation of Titanium Nickel Rotating Instruments Due to Use
Description: Deformation of Nickel-Titanium Rotating Instruments Due to Use. General Objective: to evaluate the influence of the number of uses in the deformation of nickel-titanium rotary instruments. Specific Objectives: 1. Identify the presence of deformation in nickel-titanium instruments under conditions of clinical use. 2. Identify the types of deformation in nickel-titanium instruments under conditions of clinical use. 3. To compare the frequency of deformations in the active part of Nickel-titanium endodontic instruments against different numbers of cycles of use.
Status: Completed
Nature: Research
Students involved: Undergraduate Courses: (2) / Specialization: (0) / Academic Master’s: (0) / Professional Master’s: (0) / Doctorate: (0)
Members: Juliana Melo da Silva Brandão - Member / Daniela Almeida Sousa - Member / Luciana Jorge Moraes – Coordinator
Funding: National Foundation for the Development of Private Higher Education - Other / Coordination of the Institutional Program of Scientific Initiation - Financial Aid / University Center of the State of Pará - Financial Aid
3350057 - Mario Honorato da Silva e Souza Junior (021.722.508-05)
2016 - Current
Influence of Dental Bleaching with Hydrogen Peroxide Adhesive Bonding Resistance in Enamel
Description:
Home bleaching, the currently widely used cosmetic procedure, can affect the physical and chemical properties of the restorative material, including the decrease in bond strength. The longevity of composite resin restorations is directly influenced by the stability of the adhesive bond, and the concern becomes even greater with the indiscriminate use of bleaching agents, especially by the patient in the home-made technique. It has been reported that adding fluoride and calcium to the bleaching gel can reduce enamel softening after bleaching, preserve maximum strength and reduce mineral loss of the enamel without influencing tooth whitening. The objective of this work was to evaluate the bond strength of the composite resin in bovine enamel whitened by the time recommended by the manufacturer and excessively (50% and 100%) with 4% Hydrogen Peroxide with Calcium. 120 bovine teeth will be used, which will receive standardized restorations and then divided into 4 groups: G1 (control - without bleaching), G2 (according to the manufacturer - 14 days of bleaching), G3 (excessively cleared for 21 days) and G4 (excessively cleared for 28 days). The whitening protocol will be 2 hours a day, being kept between sessions in a greenhouse at 37º, and stored in artificial saliva. After bleaching, the bond strength will be tested by means of a micro-shear test, 24 hours after the end of bleaching in each group. Excessive bleaching can significantly decrease adhesive bond strength to the enamel, compromising the quality and longevity of the restorations. Thus, evaluation of the bond strength of the composite resin in bleached enamel is important to establish an effective and safe treatment.
Status: In progress
Nature: Research.
Students involved: Academic Master’s: (2)
Members: Mário Honorato da Silva e Souza Junior - Coordinator / Sandro Cordeiro Loretto - Member / Roberta Pimentel de Oliveira - Member / Rafael Rodrigues Lima - Member / Juliana Costa Pereira Baia – Member
2015 - 2017
Do HEMA-Free Adhesive Systems Have Better Clinical Performance than HEMA-Containing Ones in Non-Carious Cervical Lesions? A Systematic Review and Meta-Analysis.
Description: OBJECTIVES: To present a systematic review in order to determine if HEMA-free adhesive systems have better clinical performance than HEMA-containing ones. SOURCES: An electronic search was performed in PubMed, The Cochrane Library, Scopus, Web of Science and Open Grey databases. The search strategy included MeSH terms, synonyms and keywords with no language or date restriction. A hand search was performed in the reference lists of included articles. STUDY SELECTION: Randomized and no randomized controlled clinical trials that compared the effectiveness of HEMA-free and HEMA-containing adhesive systems in restorations of NCCL were included. The risk of bias in the included studies was assessed and classified through the Cochrane Collaboration's common scheme for bias. Data were subgrouped and heterogeneity was tested via RevMan 5.3. DATE: A total of 2,889 potentially relevant studies were identified. After title and abstract examination, 51 studies remained and this number reduced to 25 after examination of the full-texts, which were included in the qualitative synthesis. Only 13 studies were classified as having a "low risk of bias" and were included in meta-analysis. No statistical difference was found between the clinical performances of HEMA-free and HEMA-containing adhesive systems for all parameters analized: 0.03 [-0.04, 0.09] (p = 0.42) for retention, 0.02 [-0.02, 0.06] (p = 0.25) for marginal discoloration, -0.02 [-0.07, 0.02] (p = 0.34) for marginal adaptation, 0.00 [-0.01, 0.01] (p = 0.92) for caries and -0.00 [-0.02, 0.01] (p = 0.77) for post-operative sensitivity. CONCLUSIONS: HEMA-free and HEMA-containing adhesive systems showed similar good clinical performance in restorations of NCCL.
Status: Completed
Nature: Research
Students involved: Academic Master’s: (2) Doctorate: (3)
Members: Mário Honorato da Silva e Souza Junior - Coordinator / Taise Sousa Pamplona da Silva - Member / Marcela Baraúna Magno - Member / Lucianne Copple Maia - Member / Roberta Fonseca de Castro Valino – Member
2015 - Current
Effect of Excessive Dental Whitening on the Morphology and Mechanical Properties of Dental Enamel
Description:
The search for a harmonic smile, coupled with a lack of proper professional guidance, may expose patients to excessive bleaching treatments, which can be detrimental to the integrity of the dental elements. Thus, the present study aims to evaluate the effects of excessive exposure to different modalities of bleaching treatment on the morphology and mechanical properties of dental enamel.
Status: In progress
Nature: Research
Students involved: Undergraduate Courses: (2) / Academic Master’s: (2) / Doctorate: (3)
Members: Mário Honorato da Silva e Souza Junior - Coordinator / Sandro Cordeiro Loretto - Member / Kelly Fernanda B. Vilhena - Member / Barbara Catarina Lima Nogueira - Member / Rafael Rodrigues Lima – Member
2015 - Current
Is the Bubaline Substrate a Human-Suitable Substitute in the Bonding Resistance Tests of Dental Materials?
Project certified by Coordinator Sandro Cordeiro Loretto on 10/02/2017.
Description: Since the adhesion of restorative materials to the tooth structure was achieved, where the first step was the enamel acid conditioning introduced by Buonocore (1955), this factor has represented a great achievement in the development of restorative dentistry, allowing for more conservative clinical treatments. Garcia (2007) describes that such technique makes possible the accomplishment of aesthetic, conservative and preventive restorative procedures. Since then, several generations of adhesive systems have been presented and all with the same purpose: to provide greater bond strength between the adhesive material and the dental remnant. Therefore, the need for evaluation and comparison of the adhesive capacity of the materials offered for clinical applicability is clear, given the wide range of these materials (Kadam et al., 2013; Grassi et al., 2012). According to Yanssen et al. (2013), the difficulty of obtaining sample size with quality and the impossibility of standardization (control of the source and age of the human teeth), the risks of infection and the ethical aspects involved, has made the teeth of mammalian animals used as alternatives to human teeth in in vitro and in situ studies. What had previously been recommended (Rueggeberg et al., 1991; Mellberg et al., 1992; Zero et al., 1995; Skene et al., 2002; Camargo et al., 2007). As substitutes, bovine teeth are widely used in the most diverse specialties, due to their micro and macroscopic similarity to human teeth, ease of acquisition, and low caries index. According to Schilke et al. (2000), the permanent inferior bovine incisors are frequently used due to the similarities of size and curvature with superior central incisors. The teeth of other mammalian animals (sheep, horses and pigs) have also been used as substitutes for human teeth in dental research. Nogueira et al., (2014) compared the ultrastructure and physical properties of bovine, bubaline and human enamel, concluding that the morphology, microhardness and mineral composition of the dental enamel of the animals analyzed were similar to human dental tissue. In this way, it made the bubaline substrate - enamel level - a reference for later studies. In this sense, the literature is still limited comparing the buffalo dental substrates to the human, and totally non-existent as to its use as another alternative for bond strength tests. Other clarifications must also be made regarding the morphological characteristics of bubaline dentin and to evaluate if there is similarity of this one with human dentin. According to Barbosa (2005) and Santana et al. (2011), it is mainly in the Northern region of Brazil where the buffalo herd can be found (about 50%). Thus, it is evident that, by offering the bubaline substrate as another viable and efficient alternative to the human substrate in mechanical tests, this will entail more subsidies for the accomplishment of several tests within the research in Dentistry. This research will focus on understanding the evolution of dental materials and can be performed to evaluate the bond strength of these dental substrates.
Status: In progress
Nature: Research
Students involved: Undergraduate Courses: (1) / Academic Master’s: (2) / Doctorate: (3)
Members: Mário Honorato da Silva e Souza Junior - Member / Sandro Cordeiro Loretto - Coordinator / Barbara Catarina Lima Nogueira - Member / Rafael Rodrigues Lima - Member / Juliana Costa Pereira Baia - Member / Jackelyne Noriko Kikuchi de Freitas – Member
2015 - Current
Influence of Excessive Dental Bleaching on the Resistance of Adhesive to Enamel
Description:
It is already defined that tooth bleaching can affect the quality of the adhesive interface when performed before and after making an adhesive restoration. This study aims to evaluate the effect of prolonged bleaching on the bond strength of existing restorations.
Status: In progress
Nature: Research
Students involved: Academic Master’s: (2) Doctorate: (3)
Members: Mário Honorato da Silva e Souza Junior - Coordinator / Sandro Cordeiro Loretto - Member / Rafael Rodrigues Lima - Member / Taise Sousa Pamplona da Silva - Member / Patrícia Timbó Soares – Member
2013 - 2015
Evaluation of Weak Chelating Agents in the Bond Strength of Self Adhesive Cements
Description: Dentinal surfaces will receive provisional cementations with calcium hydroxide for seven days. After the surfaces will be cleaned with pumice paste and water and treated with two EDTA and HEBP chelating agents. Then self-adhesive cements will be applied to the treated and photoactivated surfaces. CPs will undergo micro-shear tests.
Status: Completed
Nature: Research.
Students involved: Doctorate: (2)
Members: Mário Honorato da Silva e Souza Junior - Coordinator / Sandro Cordeiro Loretto – Member
2012 - 2015
Twenty-four Months Assessment of the Ethanol Molecular Adhesion Technique: a randomized clinical trial and nanodurity laboratory tests
Description:
Continuation of the clinical evaluation of restorations made in non-carious cervical lesions using pre-saturation of the dentin with gradual solutions of ethanol prior to the application of a hydrophilic primer. In parallel, the nanodness of the adhesin and MEV interface will be determined.
Status: Completed
Nature: Research
Students involved: Undergraduate Courses: (2) / Academic Master’s: (1)
Members: Mário Honorato da Silva e Souza Junior - Coordinator / Sandro Cordeiro Loretto - Member / Thais Andrade Figueiredo - Member / Patricia de almeida Rodrigues Silva e Souza - Member / Gabriela Monteiro Barbosa - Member / Kelly Fernanda B. Vilhena – Member
2010 - 2012
Clinical Study of Restorations Made in Non-Carious Cervical Cavities Saturated with Ethanol Prior to the Application of a Hydrophobic Adhesive Agent
Description:
A clinical evaluation will be performed on restorations of non-carious cervical lesions with CR and treated with acidic agent and subsequently dehydrated with ethanol application and then receiving the application of a hydrophobic adhesive agent. The aspects to be observed are marginal staining, postoperative sensitivity and retention.
Status: Completed
Nature: Research.
Students involved: Undergraduate Courses: (1) / Academic Master’s: (2)
Members: Mário Honorato da Silva e Souza Junior - Coordinator / Sandro Cordeiro Loretto - Member / Railson de Oliveira Ferreira - Member / Joyce Figueira Araújo - Member / Thais Andrade Figueiredo - Member / Patrícia A R Silva e Souza - Member.
Funding: Coordination of Improvement of Higher Level Personnel - Financial Aid / National Council for Scientific and Technological Development - Financial Aid
1153622 - Newton Guerreiro da Silva Junior (236.719.802-06)
2017 - Current
Three-Dimensional Evaluation of Condylar Alterations after Orthognathic Surgery through the Early Benefit Protocol
Description:
Condylar resorption after orthognathic surgery can be defined as a progressive alteration in the shape and volume of the mandibular condyle, being a multifactorial process that may be associated with surgical or non-surgical factors. These effects have a higher incidence in cases of bimaxillary surgeries and, possibly, related to risk factors. Methodology: The method will describe the condylar alterations after the orthognathic surgeries of 20 patients that are part of the database of the Federal University of Pará, through the comparison and overlap of digitized images obtained from computed tomography at two different time points, which are: the preoperative and the postoperative of 90 days. The software used will be Meshlab® (Meshlab, Open source, http://meshlab.net) for alignment, Autodesk (autodesk, http://www.autodesk.com.br) for virtual planning and Cloudcompare® (Cloudcompare, open source, http://www.daneilgm.net) to overlap and calculate the discrepancies using the closest interactive point (ICP) by surface mapping and the Bland-Altman statistical method. Each surface deviation will be represented on the scale graded by color. Two groups will be created: Group A, composed of patients operated with the Early Benefit protocol and Group B, control group, represented by patients submitted to the conventional surgical technique. Proposal: This is a randomized, retrospective clinical study in a database of patients with dentofacial deformity, which aims to evaluate mandibular condyle changes in patients undergoing Orthognathic Surgery through Early Benefit.
Status: In progress
Nature: Research
Members: Newton Guerreiro da Silva Júnior - Coordinator / Flávio Henrique Real - Member / José Thiers Carneiro Júnior - Member / Douglas Voss de Oliveira – Member
2015 - Current
The Impact of Malocclusion and Dental Loss on the Quality of Life of Amazonian Populations
Description: The epidemiological knowledge makes it possible to evaluate the interference of etiological factors in the occurrence of the disease, providing data for the planning of preventive and curative actions. Assessing the effect of oral conditions on quality of life provides important information for researchers, for the patient and for the health system, in addition to estimating the need for treatment. Because it is a public health problem, it is necessary to understand the biopsychosocial aspects of malocclusion and its effect on individuals' quality of life. The study of the quality of life in orthodontic patients is of fundamental importance in understanding the impact of malocclusions in their daily life, especially in relation to functional limitations and psychosocial well-being, besides making possible the planning of strategies of action and destination resources where their employment will be most effective. There is currently no data to evaluate the relationship between malocclusion, dental losses and quality of life in the indigenous, riverine and urban communities of the Amazon region.
Status: In progress
Nature: Research.
Members: Newton Guerreiro da Silva Júnior - Member / Antônio David Corrêa Normando - Member / João de Jesus Viana Pinheiro - Coordinator / Helder Henrique Costa Pinheiro - Member / Gustavo Antônio Martins Brandão – Member
2015 - Current
Tomographic Evaluation of Dental and Skeletal Changes in the Rapid Expansion of the Surgically Assisted Maxilla. Comparison between Transpalatal Bone Dystoric and Supported Dento Circuit Breaker
Description:
Theoretical basis and guiding questions: The indications for surgically assisted rapid expansion of the maxilla are skeletal maturity, transverse maxillary hypoplasia, either uni or bilateral and narrow buccal corridor. The treatment is a combination of surgical procedure and orthodontic treatment provided by means of osteogenic distraction, providing space in the dental arch for the alignment of the dentition. The procedure causes a substantial enlargement of the maxillary base and the palatine vault. Traditionally, an orthodontic appliance called the Hyrax type expander is placed preoperatively to expand the maxilla through dental anchorage, however, such anchoring may cause several undesirable side effects, including damage to the dentition, possible loss of anchorage, compression of the periodontium, root resorption, cortical fenestration. To avoid these complications, several bone-anchored devices (transpalatal distractors) have been developed. These are installed directly into the palatine bone bilaterally during surgery. It is claimed that they avoid several problems encountered with hyrax. The advantage of bone-supported devices is that the forces are applied at the desired level of position, avoiding dental vestibularization, in addition to maintaining a minimal segmental inflection. Formulation of the objectives: To evaluate the difference between the two groups, considering the amount of recurrence, the amount of displacement of the maxilla and the bone quality formed.
Status: In progress
Nature: Research.
Members: Newton Guerreiro da Silva Júnior - Coordinator / José Thiers Carneiro Júnior – Member
2015 - Current
Clinical and Radiographic Effects of the Third Molars on the Dental Arch, Periodontium and Quality of Life: parameters to indicate the need for intervention
Description:
The third molars are the teeth that are most frequently impacted, with prevalence between 9.5% and 39.5% in different populations. However, 75% of people who receive dental treatment on a regular basis have third molars removed. The decision of removal or proservation of the third molars still raises doubts to the clinician. In part, due to the inaccuracy of the models of prediction of impaction reported in the literature, and also to the scientific works with inconclusive results. Thus, this decision seems to be centered on a preference for each specialty, rather than clinical conduct based on scientific evidence. In the face of so many controversies, the prophylactic removal of the third molars has been adopted under the pretext of preventing future damage associated with various pathologies. Tertiary crowding in adults or the ultimate instability of orthodontic treatment have been associated with the presence of lower third molars, despite the fact that most studies suggest that third molars have an insignificant influence on changes in the lower arch in the long term. The most conservative approach is the careful monitoring of asymptomatic third molars. This conduct is mainly based on the absence of scientific evidence to justify the indication of prophylactic extraction. The objectives of this project are to: examine the ability of specialists to predict the eruption of third molars; to evaluate the impact of the behaviors adopted on the patients' quality of life - Risks, benefits and impact of the third molars Exodontics; to examine the changes produced by the third molars in the morphology of the dental arch; Epidemiology of third molars impactation in remote Amazonian populations and the importance of CT in the diagnosis, prognosis and proservation of third molars.
Status: In progress
Nature: Research
Members: Newton Guerreiro da Silva Júnior - Coordinator / Antônio David Corrêa Normando - Member / Gustavo Antônio Martins Brandão - Member / ARMANDO Rodrigues Lopes Pereira Neto - Member / José Thiers Carneiro Júnior - Member
2170818 - Oscar Faciola Pessoa (257.032.422-15)
1640163 - Patricia de Almeida Rodrigues (292.219.002-15)
2017 - Current
Study of the Actions of the Irrigation Protocols and Agents in the Areas of Internal Dental Resorption
Description:
To study the efficacy of protocols and irrigation agents in the removal of intra-canal medication, dissolution of organic material and fracture resistance in teeth with internal resorption.
Status: In progress
Nature: Research
Students involved: Undergraduate Courses: (3) / Academic Master’s: (1) / Professional Master’s: (1)
Members: Patricia de Almeida Rodrigues - Coordinator / Victor Feliz Pedrinha - Member / Ana Raquel Lopes dos Santos Miranda – Member
2017 - Current
Association between Sickle Cell Anemia and Pulp Necrosis in Patients with Age Groups of 6 to 12 Years Old
Description:
Asymptomatic pulpal necrosis in permanent teeth with clinically intact dental crown has been suggested in adult patients with sickle cell anemia. The relevant literature does not reveal data between the relationship of this pulp pathology in children, so this study will evaluate the association between sickle cell anemia and pulpal necrosis in individuals with different age groups. Patients registered in the Hematology and Hematology sector of PARÁ (HEMOPA) will be included. The non-exposed group will be composed of patients from the University Center of the State of Pará (CESUPA) odontological clinic that does not have sickle cell anemia, sickle cell trait, or any other systemic condition, the hemoglobin electrophoresis test will be performed to diagnose with abnormal hemoglobins. The inclusion criteria will be: minimum age of six years and the presence of at least one permanent tooth with a clinically intact dental crown. Pulp necrosis of teeth will be determined by Oximetry and by cold thermal testing. A pulse oximeter adapted to measure SaO2 from the dental pulp will be used. First, the percentage of SaO2 will be measured on the right index finger of each participant and then the SaO2 percentage of the selected permanent tooth pulp. For teeth where the oximeter readings are null, it will be considered without pulp vitality. The cold thermal test will be performed 5 minutes after pulse oximetry. A microbrush soaked with dichlorodifluoromethane gas will be applied to the cervical portion on the buccal surface of the tooth for 4 seconds. The participant will raise his hand immediately upon perceiving the sensitivity to the cold and will lower it by reducing the pain. Pulp necrosis will be considered when the patient remains insensitive to cold. Co-variables: self-reported ethnicity; sex; socioeconomic class; history of orofacial trauma; and the use of folic acid. The prevalence of necrosis with a 95% confidence interval (CI) will be calculated for both groups and compared with the chi-square and Kruskal-Wallis tests (P <0.05). The Spearman correlation test will be used to compare the two methods of evaluation of the pulp condition.
Status: In progress
Nature: Research
Members: Patricia de Almeida Rodrigues - Coordinator / Camila Ribeiro Ribeiro - Member / Larissa Dias Alexandrino - Member / Taiane Santos da Silva – Member
2016 - 2018
Influence of Etidronate on Microhardness and Dentin Roughness
Description:
Many chemical substances can be used during the biomechanical preparation of the root canal, in order to completely remove Smear Layer. Among these chelating substances, we highlight the ethylenediaminetetraacetic acid (EDTA) and Etidronate (HEPB). This work aims to analyze the influence of the use of HEPB on the roughness and microhardness of the root dentin through the analysis of thirty half-roots that will be randomly divided into three groups. Group 1 will be immersed in 40 ml of 18% HEPB solution mixed with 2.5% NaOCl. Group 2 will be placed in a solution of 40 ml of 2.5% NaOCl for 30 minutes, simulating the instrumentation, and then be submerged in a solution of 9% HEPB for five minutes simulating final washing. After this, the G2 will be immersed again in a 40ml solution of 2.5% NaOCl for fifteen seconds. Group 3 will be immersed in a solution of 40 ml of 2.5% NaOCl for 30 minutes, simulating the instrumentation process. After this procedure, the solution will be immersed in 40 ml of solution containing EDTA 17% for three minutes. In the last step, the sample will be immersed in 40 ml of solution containing 2.5% NaOCl for 15 seconds. From these procedures will be made the comparisons between the groups using the Student's t-test (p <0.05) and (ANOVA) (<0.05) for microhardness analysis. In the case of roughness, the tests used will be Wilcoxon (<0.05) and Friedman test (<0.05).
Status: Completed
Nature: Research
Students involved: Undergraduate Courses: (1) / Academic Master’s: (1)
Members: Patricia de Almeida Rodrigues - Member / Oscar Faciola Pessoa - Coordinator / Juliana Melo da Silva Brandão – Member
2016 - 2018
The Use of the Video Lesson as a Teaching Resource in Dentistry
Description:
The objective of this study is to evaluate the influence of a video lesson as a learning tool in undergraduate students of the Dentistry course. Volunteers from 1st to 6th period will be divided into three groups according to the knowledge regarding the proposed study topic: Suture applied to Dentistry. Group 1: Students who did not attend college suturing techniques; Group 2: Students who have had suture classes in college; Group 3: Students who did not have a suture class in college, but who already had some contact with the subject in a theoretical or practical way, in complementary activities to the college. Students will receive a kit containing an incised bullock tongue and instruments to perform the suture test, which will be done in three moments (before, after and during the video lesson). At each moment will be computed the time of execution of the test of each volunteer and photographs will be taken in order to evaluate the correctness and error in the execution of the requested techniques. The results obtained will be tabulated and statistically calculated.
Status: Completed
Nature: Research
Students involved: Undergraduate Courses: (1) / Professional Master’s: (2)
Members: Patricia de Almeida Rodrigues - Coordinator / Larissa Dias Alexandrino - Member / Ana Paula Guerreiro Rodrigues Couto - Member / Marcio Ruffel Cristino - Member / Thais Helena de Souza Almeida – Member
2016 - 2016
Modeling, Extrusion of Apical Debris and Related Defects Associated with Automated Systems: critical literature review
Description:
The objective of this review was to provide a detailed analysis of the literature on the correlation between different motion kinematics and modeling ability, apical extrusion of debris and dentin defects from endodontic preparation with nickel-titanium rotary instruments (NiTi). From March 2016 to January 2017, electronic searches were conducted in the PubMed and SCOPUS databases systematically for papers published since January 2010. In addition to electronic research, manual searches were made to include references listed as high-published articles impact and were not indexed in the databases. Only in vitro studies performed on human teeth that were in the English language were considered for this review. Based on the inclusion criteria, 71 papers were selected for analysis of full-text copies. Specific analysis was carried out from 44 articles describing the effects of reciprocating, continuous and adaptive movements on WaveOne Gold, Reciproc, HyFlex CM and Twisted File Adaptive systems respectively. A wide range of assay conditions and methodologies were used for comparative analysis between systems. Due to controversies among the results, the characteristics of the instruments seem to interfere with the design and the alloys, in addition, laboratory studies are limited when comparing the kinematics of the systems. Further in vivo studies are needed for further comparisons.
Status: Completed
Nature: Research
Members: Patrícia de Almeida Rodrigues - Coordinator / Brenna Magdalena Lima Nogueira - Member / Victor Feliz Pedrinha – Member
2016 - 2016
Analysis of Radicular Dentin Permeation Promoted by Different Concentrations of Sodium Hypochlorite
Description: Radial instrumentation promotes the formation of smear layer that acts as a physical barrier reducing dentin permeability. The use of certain irrigating substances increases the permeability of the dentin, aiding in the penetration of irrigating substances, intracanal medication and the sealing materials. The objective of the work plan was to evaluate the root dentin permeability when the concentration of NaOCl in 1%, 2,5%, 5,25%. Roots of bovine teeth were used. At the first moment, the roots were sectioned longitudinally and all the cement removed, and a second cross section was made. The samples were subdivided into: G1? immersion with NaOCl 1% and final wash with 17% EDTA; G2 - immersion with NaOCl 2.5% and final wash with 17% EDTA; G3 - immersion with 5.25% NaOCl and final wash with 17% EDTA; G4 - immersion with 5.25% NaOCl and final etidronate wash; G5 - immersion with 0.9% saline and final wash with 17% EDTA. The dentin samples were water-polished, washed at the end with chelating agent and immersed at different concentrations of the hypochlorite and fed to the THD-02c permeation apparatus. The fluid transport study model was used, measuring the conductance of fluid induced by hydrostatic pressure. The data were annotated in spreadsheet, being statistically evaluated by the ANOVA test to a criterion and Tukey post-test. All hypotheses were tested at a significance level of 95%. It was observed a statistically significant difference in the evaluation of the bubble displacement and the hydraulic conductance of the dentin (p <0.0001), being G1 <g5 <g2 <g3.
Status: Completed
Nature: Research
Students involved: Undergraduate Courses: (1) / Academic Master’s: (1)
Members: Patrícia de Almeida Rodrigues - Coordinator / Oscar Faciola Pessoa - Member / Brenna Magdalena Lima Nogueira - Member / Raquel de Sousa Franco Nassar – Member
2016 - Current
Application of Cone Beam Computed Tomography in the Study of Endodontic Anatomy
Description:
The project aims to evaluate and compare the parameters in the acquisition of the image and to define the best condition for identification of the number of root canals.
Status: In progress
Nature: Research
Students involved: Undergraduate Courses: (1) / Professional Master’s: (1)
Members: Patrícia de Almeida Rodrigues - Coordinator / Oscar Faciola Pessoa - Member / Camila Ribeiro Ribeiro - Member / Lucas Pinheiro - Member.
Number of counselings: 1
2016 - Current
Correlação da Anemia Falciforme com Patologias Pulpares
Description:
In patients with sickle cell anemia, mutant red blood cells are recognized by the reticuloendothelial system and destroyed, thereby reducing the cell's life span from 120 to 15 to 25 days. The accumulation of altered cells may cause vaso-occlusion; Sickle erythrocytes are more adherent than red blood cells normal to the vascular endothelium being an additional mechanism that causes vascular obstruction. All of these factors can lead to anoxia, stroke, necrosis and pain. Clinical problems include: Hemolytic anemia, bacterial infections, and painful, vast-occlusive crises may lead to ischemic necrosis of the pulpal tissue, even without other risk factors. These infections may evolve from osteomyelitis because of bone marrow hypovascularisation secondary to thrombosis. This reinforces the importance of adopting preventive measures against the adverse effects of sickle cell anemia, because there is no cure for red blood cell fouling. When pulp necrosis occurs, the root canal can become an environment conducive to bacterial planting and multiplication. The inability of defense cells to act in the root canal causes the microbial aggression to the periapical tissues to be permanent. Thus, it is important that all possible sources of chronic infection be diagnosed early in order to avoid the development of osteomyelitis (BISHOP; KELLEHER, 1995), which is present in at least 29% of patients with sickle cell disease. (KELLEHER, BISHOP, BRIGGS, 1996) For this reason, the study on the correlation between sickle cell anemia and frequency of pulpal necrosis is substantial, since this morbidity causes a great impact on patients' life expectancy (CORREA, 2016). It is also considered the importance of this research, due to the lack of clinical studies in the pertinent literature, especially regarding the age group.
Status: In progress
Nature: Research
Students involved: Undergraduate Courses: (1) / Professional Master’s: (2)
Members: Patricia de Almeida Rodrigues - Coordinator / Oscar Faciola Pessoa - Member / Camila Ribeiro Ribeiro - Member / Larissa Dias Alexandrino - Member / Taiane Santos da Silva – Member
2016 - Current
Study of Irrigating Solutions and their Effects on Root Canal Decontamination and Marginal Sealing Quality
Status: In progress
Nature: Research
Students involved: Academic Master’s: (2)
Members: Patricia de Almeida Rodrigues - Coordinator / Oscar Faciola Pessoa - Member / juliana melo da silva - Member / Lorena CAssia Oliveira Athaide da Silva - Member / Victor Feliz Pedrinha – Member
Number of counselings: 2
2016 - Current
Plantas da Amazônia Aplicadas na Terapêutica Endodôntica
Description:
Jambu (Spilanthes acmella) is a plant native to the America of the Amazon region widely used in the control of odontalgias by the local population. Studies have shown that jambu has numerous pharmacological properties, among them antimicrobial, anti-inflammatory activity and an anesthetic power comparable to classic drugs, such as xylocaine. The objective of this study will be to evaluate the antimicrobial activity of the jambu extract in different bacterial groups of the buccal flora, especially those found in the root canals.
Status: In progress;
Nature: Research.
Students involved: Undergraduate Courses: (1) / Academic Master’s: (1) / Professional Master’s: (2)
Members: Patricia de Almeida Rodrigues - Member / Oscar Faciola Pessoa - Member / Camila Ribeiro Ribeiro - Member / Larissa Dias Alexandrino - Member / Ludmylla Gomes e Silva - Member / Julyanne dos Anjos Silva Furtado - Member / Mileide da Paz Brito - Coordinator.
2016 - Current
Evaluation of Various Resin Properties Bulk Fill
Description:
The composite resins of the Bulk Fill type (BF) with the aim of simplifying the restorative procedure, since they allow the use of a single increment of up to 5mm thickness, which optimizes restorative procedures and reduces clinical time. This study aims to evaluate the various physical and mechanical properties of resin (BF): color stability, microhardness, degree of conversion, aging and surface smoothness. For this study, specimens of conventional composite resins (CC) and BF and will be randomized into groups according to the experimental study to be investigated.
Status: In progress;
Nature: Research.
Students involved: Undergraduate Courses: (1) / Professional Master’s: (4).
Members: Patricia de Almeida Rodrigues - Member / Renata Antunes Esteves - Coordinator / Camila Ribeiro Ribeiro - Member / Larissa Dias Alexandrino - Member / Carlos Augusto de Carvalho - Member / Mariana Vieira Balla - Member.
2015 - 2018
Integrity of Marginal Sealing Provided by Bioactive Endodontic Cements.
Description:
The insertion of dental materials with bioactive capacity has been highlighted by allowing a favorable biological response. However, to perform their functions they must possess adequate physicochemical properties. This study will have the main objective of evaluating the interface endodontic cement and dentin, and correlate with the quality of marginal sealing.
Status: Completed;
Nature: Research.
Students involved: Academic Master’s: (1).
Members: Patricia de Almeida Rodrigues - Coordinator / Oscar Faciola Pessoa - Member / Eliza Burlamaqui Klautau - Member / claudia pires rothbarth - Member / juliana melo da silva - Member / Lorena CAssia Oliveira Athaide da Silva – Member
2015 - 2018
Influence of Irrigation Solutions on Resistance to Radicular Fracture and Dental Permeability
Description:
The vertical root fracture (FRV – Portuguese acronym) is a longitudinally oriented fracture of the root originating from the apex and propagating to the coronal part? (1). FRV is an important clinical problem in endodontics because they are associated with poor prognosis for the affected tooth, leading to tooth extraction. In the root canal system there are spaces that cannot be cleaned mechanically, so the chemical disinfection of these spaces is responsible. This is an essential process in the treatment of canals because it has many purposes, to lubricate the walls of the root canal, reducing the mechanical stress on the endodontic instruments and preventing the separation of the instrument, antimicrobial action and removal of debris formed by the instrumentation. Despite the fact that these solutions facilitate the instrumentation of the canal, they can cause a possible reduction of the microhardness, since when the solutions are in contact with the chemical structure of the dentin, they modify the relation that exists between calcium / phosphorus of the surface. Sodium hypochlorite (NaOCl) solutions are the most common irrigant agents used in the biomechanics of the preparation based on their excellent microbicidal activity and tissue dissolving ability; there are studies that indicate that NaOCl decreases dentin microhardness at all concentrations, thus having a detrimental effect on dentin, elasticity and flexural strength; due to the proteolytic action of the hypochlorite concentration in the dentin collagen matrix. With regard to chelating agents, the effects of decalcification depend on the amount of the irrigant used, the time of application, and pH concentration. Although several studies have evaluated the effect of irrigating solutions on radicular dentin microhardness, FRV studies are necessary to analyze the effect of different irrigating solutions, whether isolated or associated with different chelating agents; since the irrigant should facilitate the disinfection and removal of the dentin, but not weaken the structure of the tooth. Therefore, the objective of this study will be to evaluate the influence of irrigation solutions on root fracture of endodontically treated teeth.
Status: Completed
Nature: Research
Students involved: Academic Master’s: (1)
Members: Patrícia de Almeida Rodrigues - Coordinator / Mario Honorato da Silva e Souza Junior - Member / Oscar Facíola Pessoa - Member / Melissa Lantigua Domingues – Member
Number of counselings: 1
2015 - 2017
Is Etidronic Acid an Inhibitory Potential for Radicular Dentine Metalloproteinases?
Description:
MMPs are a group of endopeptidases capable of degrading collagen, one of the organic constituents of dentin. Several materials have been proposed to inhibit MMPs, such as CHX 2% and EDTA 17%, but to date there are no reports of the relationship of HEBP to the enzymes observed in dentin of the root canal. Thus, this in vitro study aims to investigate the action of HEBP on the functional activities of the MMPs and cathepsin of cysteine bound to the root dentin matrix of the cervical, middle and apical thirds by means of the in situ zymography technique, mass loss of dentin and quantification of ICTP and CTX.
Status: Completed
Nature: Research.
Students involved: Undergraduate Courses: (1) / Academic Master’s: (1)
Members: Patrícia de Almeida Rodrigues - Coordinator / Juliana Melo da Silva - Member / Sandro C Loretto - Member /, Oscar Facíola Pessoa - Member / Brenna Magdalena Lima Nogueira – Member
Number of counselings: 1
2015 - Current
Epidemiological Profile of Tooth Injuries in Riveside Populations
Project certified by Coordinator Juliana Melo da Silva Brandão on 10/26/2017.
Description:
The dental trauma has importance in the reality of the population because it is among the problems of public health, being alongside caries disease and oral cancer. In Brazil, according to the latest epidemiological survey, dental trauma is considered a public health problem, which must be prevented and, when it occurs, treated systematically (SB-Brasil 2010). With a theme of such social responsibility and without great focus on the relations between the Ministry of Health and Education, the Project of Injury Trauma arises, with the purpose of informing the population about preventive measures and solving problems involving the dental element trauma in community rivers that do not have the public dental care service. The objective of this work is to carry out an epidemiological survey of the current oral health situation of the riverside population living in the islands located near the Metropolitan Region of Belém, with emphasis on dental traumatism. The sample will consist of riverside children and adolescents, living in the islands located on the banks of the Guamá rivers. Guidance will also be given on the measures to be taken in certain trauma cases, from coronary fracture involving only enamel to dental avulsion, time between trauma and relief to obtain a good prognosis, cleaning and storage of the fragment or dental element and forms of prevention. It is also intended the dissemination of dental care services at the Federal University of Pará, in order to integrate the community to UFPA and provide preventive and curative dental care to this population. The information obtained will be tabulated in gender, age group, etiological factor, type of lesion, affected teeth, stage of root development and time of control of the case. After obtaining the data, these will be tabulated and the results analyzed statistically by frequency, average and percentage estimates. Statistical treatment and significance assessment will be performed using the Chi-square test with a significance level of 5%. With the results obtained it is expected to draw up prevention strategies in order to minimize the occurrence of this accident in the riverside community.
Status: In progress
Nature: Research.
Students involved: Academic Master’s: (1)
Members: Patrícia de Almeida Rodrigues - Member / Oscar Faciola Pessoa - Member / Juliana Melo da Silva - Coordinator / Gustavo Antônio Martins Brandão – Member
2014 - 2015
Analysis of the Surface Roughness of an Experimental Material Based on Mineral Trioxide plus Acrylic Resin
Description:
The objective of this study was to evaluate the surface roughness of three restorative materials, Zinc-Eugenol Oxide (ex: IRM®); glass ionomer cements (e.g.: Maxxion R - FGM), resinous compounds (Filtek - Z350 XT 3M ESPE) and roughness (ME) based on mineral trioxide plus acrylic resin). 40 specimens were prepared from a 5-hole stainless steel plate. Each material has been manipulated according to the orientation of its respective manufacturers and accommodated in the holes. The 40 specimens were divided into 4 groups and each group containing 10 specimens. The surface roughness (Ra) was analyzed by a rugosimeter SV-3000 (Mitutoyo, Suzano, Brazil). Three measurements were performed for statistical calculation purposes. The collected data were analyzed by the Kruskal-Wallis test and Dunn's post-test to evaluate the differences between the groups. There was a statistically significant difference between the analyzed groups.
Status: Completed
Nature: Research
Students involved: Undergraduate Courses: (1) / Academic Master’s: (1)
Members: Patrícia de Almeida Rodrigues - Coordinator / Brenna Magdalena Lima Nogueira - Member / Debora Souza da Luz – Member
2014 - 2015
Cervical Barrier Conformation with a Mineral Trioxide-Based Material plus Acrylic Resin: preliminary study
Description: The preparation of the cervical barrier is indicated as one of the most relevant clinical procedures in the prevention of the occurrence of external cervical resorption in teeth that have been bleached. For research, we will use roots of unirradicular teeth of humans. The sample size, necessary for the accomplishment of the experiments of stages I and II, will be defined after the pilot studies. After the coronary access, instrumentation and radicular obturation, the teeth will be divided into four groups, according to the material used as cervical barrier: Group I (control), teeth without cervical protection; Group II, chemically activated glass ionomer (Vidrion R, SS White); Group III, MTA (Angelus, Londrina, PR, Brazil), and group IV: experimental material (ME). All barriers will have a thickness of 2mm. Next, a bleaching paste composed of 35% hydrogen peroxide associated with sodium perborate will be applied to the pulp chamber and coronary access sealed with glass ionomer. The teeth will be completely waterproofed with two layers of nail polish, except for the amelo-cement joint region. In the first step, the pH change will be evaluated.
The specimens will be submerged in 20mL of bidistilled water and the pH will be measured at its initial time, 2, 4 and 7 days. For each time will be taken 3 measures, obtaining the average for comparison between the groups. After 7 days, in the second stage of the experiment, the coronary sealing and whitening paste will be removed and the pulp chamber immediately filled with 2% methylene blue dye in aqueous solution. After 24 hours, the specimens will be sectioned longitudinally with diamond disk mounted in low rotation. The samples will be observed through a Zeiss stereomicroscope at a magnitude of 50X and recorded photographically with a digital camera (Sony Cyber-Shot DSC-W730 16.1 MP). The analysis of the penetration depth of the dye in the dentin will be performed by means of scores where: 0 (without infiltration); 1 (infiltration in 1/3 of the dentin thickness); 2 (half the dentin thickness); 3 (complete infiltration of the dye reaching the outer portion). The determination of the differences between the groups will be performed by analysis of variance ANOVA and the comparison of means by the Tukey test if the data distribution is normal, or by the Kruskal-Wallis test and the comparisons of means by the Dunn test, if the distribution is abnormal. All assumptions will be tested with a confidence level of 95%.
Status: Completed
Nature: Research
Students involved: Undergraduate Courses: (1) / Academic Master’s: (1)
Members: Patricia de Almeida Rodrigues - Coordinator / Daiane Claydes Baia da Silva - Member / Brenna Magdalena Lima Nogueira – Member
2014 - 2015
Analysis of the Radiopacity of an Experimental Material Based on Mineral Trioxide plus Acrylic Resin, Comparing it to Four Restorative Materials
Description:
This work aimed to analyze the radiopacity of an Experimental Material, comparing it to four restorative materials, using a digital radiography system. For this, the materials were divided into 5 groups, 5 specimens measuring 1x10mm were prepared for each group, stored at 37 °C for 24 hours. The specimens and an aluminum scale, used as a parameter of radiopacity, were positioned in the central region of the sensor of the digital system (Digora), the cylinder of the radiographic apparatus was positioned perpendicularly, with focal length of 20cm and exposure time of 0 , 4 sec. From the digital image, the values of the optical density were obtained in pixels of the central region and 2 equidistant points. Data were analyzed by ANOVA and Tukey's test (α = 0.05). There was a statistical difference between all materials, except between zinc oxide and eugenol and Coltosol. It was concluded that the experimental material has adequate radiativity.
Status: Completed
Nature: Research
Students involved: Undergraduate Courses: (1) / Academic Master’s: (1)
Members: Patrícia de Almeida Rodrigues - Coordinator / Brenna Magdalena Lima Nogueira - Member / Maiza Cristina Preira Soares – Member
2014 - 2015
Evaluation of Wettability of Root Dentin Treated with Different Chemical Substances
Description:
Knowing that irrigation solutions cause changes in the surface of the human dentine 35,36, that the endodontic cement must be in close contact with the substrate to allow chemical adhesion or surface penetration for micromechanical fixation 37, and that the wettability is influenced by the chemical composition, roughness and density and orientation of dentinal tubules 38, it is important to evaluate the wettability of endodontic cements in contact with treated dentin with irrigation protocols already accepted in the literature and recent proposals, such as the use of weak chelants and added substances of surfactants, in the different thirds radicular.
Status: Completed
Nature: Research
Students involved: Undergraduate Courses: (1) / Academic Master’s: (1)
Members: Patricia de Almeida Rodrigues - Coordinator / Oscar Faciola Pessoa - Member / Kawane Ferreira Moraes Medeiros - Member / Marcela Magno – Member
2013 - 2014
Evaluation of the Proximity of the Root Apices with the Sinusal Membrane of Endodontically Treated Teeth with and Without Periapical Injury
Description: Considering the proximity between the maxillary sinus and the root apex of the second premolars and the first and second maxillary molars, this work, using cone-beam computed tomography (CT) images, has the objective of classifying the relation of the roots of 696 teeth with the the maxillary sinus floor and to evaluate the correlation between sinus membrane thickening and apex distance, endodontic treatment and periradicular lesion.
Status: Completed
Nature: Research
Students involved: Undergraduate Courses: (1) / Academic Master’s: (1)
Members: Patricia de Almeida Rodrigues - Coordinator / claudia pires rothbarth - Member / Luciana Moraes - Member / Fabricio de Mesquita Tuji - Member / Vitor Vilhena Pinheiro - Member / Daiane Claydes Baia da Silva - Member.
Number of counselings: 1
2013 - 2014
Evaluation of the influence of Apical Enlargement on the Transportation of Prepared Radiary Channels with Wave On System
Description:
The objective of this study was to evaluate the influence of apical debridement on root canal transport, as well as to compare the influence of apical debridement performed with PathFile (Denstisply, Maillefer, Ballaigues, Switzerland) and with manual instruments on channels instrumented by the rotational wave system One (Dentsply-Maillefer, Ballaigues, Switzerland).
Status: Completed
Nature: Research
Students involved: Undergraduate Courses: (2) / Academic Master’s: (1)
Members: Patricia de Almeida Rodrigues - Coordinator / Vitor Vilhena Pinheiro - Member / Daiane Claydes Baia da Silva - Member / Mona KAila Carvalho dos Santos – Member
2012 - 2013
Effect of Dexamethasone on Endodontic Pain Control in Single or Multiple Sessions
Status: Completed
Nature: Research
Students involved: Academic Master’s: (1)
Members: Patricia de Almeida Rodrigues - Coordinator / Oscar Faciola Pessoa - Member / Talita Tartari – Member
2010 - 2012
Removal of Smear Layer and Its Influence on Side Canal Obstruction
Description:
The success of endodontic treatment depends on three fundamental factors: sanification, modeling and hermetic filling of the root canal system. In this last one, the function is to fill the voids created during the enlargement and modeling of the canal and that previously was occupied by the dental pulp. During the phase that precedes root canal filling, there is the smear layer produced by the action of the cutting instruments applied during the biomechanical preparation of the root canal. These smears can form the smear plugs, which arise as a consequence of the displacement of the smear layer into the dentinal tubules, forming a kind of tampon. The presence of this layer means an obstacle to the flow of the sealant, which may interfere with its penetration into root canal systems, especially lateral canals and adhesion to the dentin, with a negative impact on the quality of the obturation. Considering the aspects related above, the objective is to compare whether or not the smear layer removal method provides a better filling of lateral channels and which filling technique fills the lateral channels more adequately.
Status: Completed
Nature: Research
Students involved: Undergraduate Courses: (1) / Academic Master’s: (1)
Members: Patrícia de Almeida Rodrigues - Coordinator / M. H. Silva e Souza - Member / Marcela Araújo Rodrigues – Member
Funding: Paraense Amazon Foundation for Research Support - Scholarship
2008 - 2010
Evaluation of Bacterial Infiltration in Radicular Canals Obturated by Single Cone
Status: Completed
Nature: Research.
Students involved: Undergraduate Courses: (2) / Academic Master’s: (1)
Members: Patrícia de Almeida Rodrigues - Coordinator / Eliza Burlamaqui Klautau - Member / Antônio Maneschy Faria – Member
Funding: Paraense Amazon Foundation for Research Support - Financial Aid
2445539 - Rafael Rodrigues Lima (649.591.042-87)
2016 - Current
Investigation of the Activity of Metalloproteinases (MMP-2 and MMP-9) in Coronary and Radicular Dentin: a segmental study in human teeth
Description:
Project funded by Public Notice No. 006/2016 Research Aid and Grant of Academic Master’s Scholarships for Research Aid and Grants of FAPESPA’s Academic Master’s Scholarship. In this Public Notice, funding was obtained for master's dissertations and masters scholarships for the Dentistry Graduate Program of UFPA – PPGO – UFPA.
Status: In progress
Nature: Research
Students involved: Academic Master’s: (2)
Members: Rafael Rodrigues Lima - Coordinator.
Funding: Federal University of Pará - Financial Aid
2015 - Current
Analysis of the Biochemical, Genotoxic Effects and Proteomic Profile of the Peripheral Blood, Central Nervous System and Salivary Glands of Rodents Chronically and Systemically Exposed to Fluorides
Description:
This project aims to investigate the effects of chronic and systemic exposure to fluoride, in concentrations similar to that of fluoridated water supply, as well as water from endemic regions of fluorosis, simulating those reported in rodent exposures. Although the effects of fluoride on reducing the incidence and activity of caries disease are unquestionable, much remains to be elucidated about the risks and effects on biological systems when exposed to fluorides for long periods and in a systemic way. For this, this project proposes to map several anatomical regions, through several parameters, such as: the peripheral blood antioxidant system; in the antioxidant system, genotoxic analysis and proteomic profile of salivary glands as well as on the cognitive and motor functions, biochemical and proteomic modulations of several brain regions.
Status: In progress
Nature: Research
Students involved: Undergraduate Courses: (4) / Doctorate: (1)
Members: Rafael Rodrigues Lima - Coordinator / Marta Chagas Monteiro - Member / Giza Hellen Nonato Miranda - Member / Roberta Souza D'Almeida Couto - Member / Géssica de Oliveira Lopes - Member / Leonardo de Oliveira Bittencourt - Member / Walessa Alana Bragança Aragão - Member / Leidiane Alencar de Oliveira Lima – Member
2015 - Current
Changes in the Periodontium and Salivary Glands of Rats Treated with Ethanol
Description:
To investigate, through tissue / immunohistochemical analysis and oxidative biochemistry, the changes promoted by chronic or intermittent exposure to ethanol at different doses, time and age for the beginning of exposure in rats.
Status: In progress
Nature: Research.
Members: Rafael Rodrigues Lima - Coordinator / Sérgio M Alves Jr - Member / João de Jesus Viana Pinheiro – Member
2015 - Current
Evaluation of Tissue, Biochemistry and Proteomics of Salivary Glands in Animal Models of Metal Exposure
Description: This project aims to evaluate the effects of systemic exposure to potentially toxic metals such as mercury, aluminum, lead and cadmium, common in mining or mineral exploration areas in the Amazon. The mining activity, legal and clandestine, together with the waste dumped in the rivers from the large mining companies make the Amazon region a region where toxiciological damages from exposure to metals can be considered endemic, being one of the greatest challenges in public health. The objective is to simulate the toxicological damages caused by environmental exposure to rodent salivary glands. For this purpose, a variety of toxicological evaluation parameters will be used, from the measurement of metal deposits in the glandular parenchyma, analysis of oxidative biochemistry, proteomic evaluation and tissue / immunohistochemical characterization of the reaction and / or tissue components damage in salivary glands.
Status: In progress
Nature: Research
Members: Rafael Rodrigues Lima - Coordinator / Sérgio M Alves Jr - Member / Railson de Oliveira Ferreira - Member / João de Jesus Viana Pinheiro - Member / Priscila Cunha Nascimento - Member / Nathália Carolina Fernandes Fagundes - Member / Géssica de Oliveira Lopes - Member / Leonardo de Oliveira Bittencourt - Member / Walessa Alana Bragança Aragão - Member / Bruna Puty - Member / Leidiane Alencar de Oliveira Lima - Member / Mario Teixeira Maneschy – Member
2014 - 2016
Moderate Aerobic Physical Training as a Neuroprotective Strategy for Ischemic Vascular Accident in Motor Cortex of rats: motor, tissue and oxidative stress markers evaluation
Description:
This project aims to investigate the effects of moderate aerobic physical training on the possible neuroprotection / minimization of damage after ischemic stroke in Wistar rats. For this, the animals will be submitted to a treadmill running protocol for 10 weeks, and at the end of this period, focal ischemia will be induced in the motor cortex. On the third, seventh and thirtieth day after ischemia, the animals will be submitted to a series of behavioral tests that evaluate the motor performance, being then sacrificed and the brains evaluated the damage by tissue immunohistochemistry as well as the volume of injury or by oxidative stress markers. Data will be tabulated and compared with data from animals not submitted to training, whether they have ischemia or not. Project approved and finalized by Public Noitce 007/2013 – First Projects Program - PPP - FAPESPA / CNPq.
Status: Completed
Nature: Research
Students involved: Undergraduate Courses: (3) / Academic Master’s: (3) / Doctorate: (2)
Members: Rafael Rodrigues Lima - Coordinator / Luanna de Melo Pereira Fernandes - Member / Luana de Nazaré Silva Santana - Member / Francisco Bruno Teixeira - Member / Cristiane do Socorro Ferraz Maia - Member / Marcio Antonio Raiol dos Santos - Member / Marta Chagas Monteiro - Member / Paulo Mecenas Farias-Júnior – Member
Funding: UFPA - Financial Aid
2014 - 2016
Characterization of Injury in the Motor Cortex of Mice after Ischemic Vascular Accident in a Model of Chronic Intoxication with Methylmercury: histopathological, motor, electrophysiological and oxidative stress markers
Description:
Exposure to mercury is related to changes in the central nervous system. This study will develop an experimental model of chronic exposure to low doses of methylmercury prior to a stroke, so that injuries can be studied in the motor cortex of rats after ischemic stroke in chronically exposed animals. For this, the 60 animals will be used, of which some will be intoxicated with methylmercury, orally, by gavage, at the dose of 0.04mg / kg / day for 60 days. They will then undergo focal ischemia with endothelin in the motor cortex. After 7 days, they will be submitted to behavioral tests that evaluate motor performance, evaluated for their electrophysiological parameters through registers and then their collected brain for histopathological / immunohistochemical analysis, oxidative stress and mercury deposits in this brain region. The data will be evaluated seeking the understanding of the comorbidity of these injuries. Project approved and financed by Public Notice No. 006/2014 Program of Research Assistance – Thematic Projects of FAPESPA: Health.
Status: Completed
Nature: Research
Students involved: Undergraduate Courses: (4) / Academic Master’s: (2) / Doctorate: (1)
Members: Rafael Rodrigues Lima - Coordinator / Márcia Cristina Freitas da Silva - Member / Cristiane do Socorro Ferraz Maia - Member / Marta Chagas Monteiro - Member
Funding: Paraense Amazon Foundation for Research Support – Financial Aid
2014 - 2016
Neurobehavioral Effects and Oxidative Biochemistry of Chronic Exposure to Ethanol and Methylmercury in the Period of Adolescence in Female Rats
Description:
Mercury is a heavy metal responsible for acute intoxication, and in the most significant form, chronic intoxication in man resulting in damage to the central nervous system (CNS). The use of mercury in the artisanal mining activity is necessary for the formation of the amalgam in the extraction of the gold, facilitating the identification of the metal, which is then subjected to heating, the mercury being eliminated as vapor to the atmosphere, depositing itself in the rivers, and by bacteria action becomes organic (methylmercury-MeHg) or remains in the biological cycle contaminating fish and crustaceans, resulting in chronic intoxication for the population exposed to steam or consuming contaminated food. Besides mercurial intoxication, there is another associated risk, the high consumption of alcohol in the mining regions, which has been studied due to the neurological damages caused by the abuse of its consumption. Epidemiological studies have shown that alcohol consumption has increased, especially among adolescents and women, but the effects resulting from the association of these two toxicants in adolescence are obscure. The objective of this study will be to investigate the cognitive and behavioral changes resulting from the simultaneous exposure of alcohol and MeHg. Wistar rats (n = 40) will receive water, ethanol (EtOH), MeHg and EtOH + MeHg for 30 days, from 30 to 60 days of age (adolescence) by gavage. After treatment (24 Credit Hours), the animals will be submitted to tests of locomotor activity, labyrinth in raised cross, forced swimming and inhibitory avoidance to evaluate the behaviors related to anxiety, depression and mnemonics. After the behavioral tests, blood samples will be collected for analyzes of oxidative biochemistry. Project approved and financed by Public Notice Nº 006/2014 Program of Research Assistance – Thematic Projects of FAPESPA: Health.
Status: Completed
Nature: Research
Students involved: Undergraduate Courses: (2) / Academic Master’s: (2)
Members: Rafael Rodrigues Lima - Member / Enéas de Andrade Fontes - Member / Cristiane do Socorro Ferraz Maia - Coordinator / Marta Chagas Monteiro - Member.
Funding: Paraense Amazon Foundation for Research Support - Financial Aid
2014 - Current
Investigation of the Effects of Physical Training of Moderate Intensity on the Motor and Cognitive Parameters of Rats Chronically Exposed, in an Intense or Episodic Ways (Binge), to Ethanol, from Adolescence to Adulthood
Description: In this project, we aim to investigate the effects of moderate intensity physical training on the motor and cognitive, tissue and biochemical parameters of rats chronically exposed to ethanol, in an intense and episodic way (binge), from adolescence to adulthood. Project approved by the CNPq Universal Notice, called MCTI / CNPQ / Universal 14/2014 – Banner A 2014.
Status: In progress
Nature: Research
Students involved: Undergraduate Courses: (2) / Academic Master’s: (1) / Doctorate: (2)
Members: Rafael Rodrigues Lima - Coordinator / Luanna de Melo Pereira Fernandes - Member / Luana de Nazaré Silva Santana - Member / Cristiane do Socorro Ferraz Maia - Member / Marta Chagas Monteiro - Member / Francisco Bruno Teixeira - Member / Russell Santiago Correa - Member / Paulo Mecenas Farias-Júnior – Member
2014 - Current
Characterization of the Alterations in the Motor, Hippocampus and Cerebellum Cortex of Adult Rats Submitted to Chronic Intoxication with Inorganic and Organic Mercury
Description:
This project aims to evaluate the effects of chronic and low dose exposure to methylmercury and mercuric chloride on behavioral aspects of cognition and motor performance, tissue, apoptotic profile, viability and cell death, proteomics and biochemical on the central nervous system, comparing the susceptibility of three different areas: motor cortex, hippocampus and cerebellum of rats. For this, rats will be poisoned for 45 days at a dose of 0.375 mg / kg / animal with Mercury Chloride (HgCl2) and for 60 days at a dose of 0.04 mg / kg / animal with Methylmercury (MeHg), doses reported in the literature as capable of promoting mercury deposits in the neural parenchyma. At the end of these periods, the animals will be sacrificed and the central nervous system regions evaluated for the amount of total mercury deposited in the nervous tissue in these areas, possible changes in neuronal and glial density, as well as changes in viability / apoptosis / cell death, proteomic, biochemical and neurochemical.
Status: In progress
Nature: Research
Students involved: Undergraduate Courses: (2) / Academic Master’s: (1) / Doctorate: (1)
Members: Rafael Rodrigues Lima - Coordinator / Márcia Cristina Freitas da Silva - Member / Luana de Nazaré Silva Santana - Member / Francisco Bruno Teixeira - Member / Cristiane Socorro Ferraz Maia - Member / Paulo Mecenas Farias-Júnior - Member / Leonardo de Oliveira Bittencourt – Member
Number of S, T & A Productions: 1
2013 - 2015
Morphometric, Immunohistochemical and Oxidative Stress Markers in Salivary Gland of Mice after Chronic Poisoning with Mercury and Methylmercury Chloride
Description:
This project aims to evaluate the effects of chronic exposure and low dosage of methylmercury chloride and mercury chloride in rats' salivary glands. For this, the rats will be intoxicated for 45 days at a dose of 375mg / kg / animal with either Metimerium Chloride (MeHgCl) or Mercury Chloride (HgCl2). At the end of this period, the animals will be evaluated for the amount of mercury deposited in the glandular tissue, possible histopathological changes, immunohistochemical changes and changes in markers of oxidative stress. Project approved and financed by the PROPESP Public Notice 06/2013 – Researcher Support Program; Pro-doctor 2013 / Subprogram Support to the New Doctor; PARD.
Status: Completed
Nature: Research
Students involved: Undergraduate Courses: (2)
Members: Rafael Rodrigues Lima - Coordinator / Márcia Cristina Freitas da Silva - Member / João de Jesus Viana Pinheiro - Member / Marta Chagas Monteiro - Member
Funding: Federal University of Pará – Scholarship
2013 - 2015
Extracts from Amazonian Plants and their Active Principles as Anti-Inflammatory and Neuroprotective Agents in Experimental Models of Stroke and Cranioencephalic and Spinal Cord Trauma
Project certified by Coordinator Walace Gomes Leal on 05/05/2014.
Description:
Acute and chronic neurodegenerative diseases remain untreated. In the case of stroke, which together with trauma to the spinal cord and brain, is the third leading cause of death in the world, and the first in Brazil, there are no effective pharmacological or cellular therapies, with the exception of the enhancer activator. recombinant tissue plasminogen (alteplase) that benefits few people because of its limited therapeutic window and risk of hemorrhagic transformation. For trauma, high doses of methylprednisolone are used in humans, but also with little efficacy. The inefficacy of available therapies legitimizes the search for alternative neuroprotective agents. Considering that the Amazon is an unexplored barn of phytotherapics with potential neuroprotective and / or anti-inflammatory effects, in this project we will investigate the effects of extracts of four plants considered by popular medicine as beneficial for diseases of the central nervous system (CNS): copaiba ( Copaifera reticulata Ducke), black sesame (Sesamum indicum L), jucá (Caesalpinia ferrea Martius) and cipó puçá (Cissus sicyoides L). Animals (adult rats) will be submitted to ischemia in different regions of the CNS or trauma to the spinal cord or cranioencephalic. They will be treated with the plant extracts mentioned in different concentrations and will be perfused at different survival times for the first month after the induction of the lesion. Control animals will be treated with 5% tween, the diluent of the extracts. The extracts will be isolated by supercritical fluid extraction. After histological processing, the sections will be stained for cresyl violet and immunolabelled for neurons, microglia, astrocytes, oligodendrocytes, lymphocytes and pathological axons. The effects of treatments on the histopathological patterns mentioned, including volume of infarction, will be quantitatively analyzed. Specific behavioral tests will be used to evaluate functional recovery. The difference between the groups will be evaluated by ANOVA-Tukey. The possible active principles will be isolated by high performance chromatography. With this study, we intend the isolation of phytotherapics with anti-inflammatory and or neuroprotective action for acute CNS diseases. We intend to place on the market these new neuroprotective and / or anti-inflammatory agents. Project approved and financed by Public Notice 001/2013 – Support to the development of a network of research in health – herbal and phytotherapeutic plants – FAPESPA.
Status: Completed
Nature: Research
Members: Rafael Rodrigues Lima - Member / Wallace Gomes-Leal - Coordinator / Antonio Pereira Júnior – Member
Funding: UFPA - Financial Aid
2013 - 2015
Changes in the Central Nervous System and Stomatognathic Apparatus of Chronically Treated Mice with Adult Ethanol of Adolescence
Description: Investigate through tissue analysis, behavioral and enzymatic antioxidant system changes in the Central Nervous System and stomatognathic apparatus of rats chronically treated with ethanol from adolescence to adulthood.
Status: Completed
Nature: Research
Students involved: Academic Master’s: (2) Doctorate: (2)
Members: Rafael Rodrigues Lima - Coordinator / Luanna de Melo Pereira Fernandes - Member / Luana de Nazaré Silva Santana - Member / Cristiane do Socorro Ferraz Maia - Member / Rafael Monteiro Fernandes - Member / Rui Daniel Prediger - Member / Francisco Bruno Teixeira - Member / Paulo Mecenas Farias-Júnior - Member / Maria Helena Crespo-López - Member.
Number of S, T & A Productions: 3
2013 - Current
Characterization of the Ultrastructure and Physical Properties of Dental Tissues of Wild Animals and Productive Chain Animals as Possible Replacements to Human Teeth with Applicability in Laboratory Research
Description:
This project seeks to find animal alternatives to replace human teeth in laboratory researchs. This research is based on different methodological approaches, from ultra - structural studies to scanning microscopy (qualitative and quantitative), as well as the study of physical properties such as strength, microhardness and roughness.
Status: In progress
Nature: Research
Students involved: Undergraduate Courses: (1) / Academic Master’s: (2) / Doctorate: (2)
Members: Rafael Rodrigues Lima - Coordinator / Barbara Catharina Lima Nogueira - Member / Natália Carolina Fernandes Fagundes - Member / Francisco Bruno Teixeira - Member / Micaele Maria Lopes Castro - Member / Raira Brito Silva – Member
Number of S, T & A Productions: 2
2012 - 2015
Preclinical and Clinical Studies on Stroke (Cerebrovascular accident) in the Paraense Amazon: epidemiology, neuroprotective agents and indicators of neurological prognosis in patients with ischemic and hemorrhagic stroke
Project certified by Coordinator Walace Gomes Leal on 05/14/2013.
Description: This project aims to investigate the epidemiological profile of the Stroke in the main cities of Pará, allied to a clinical study with the objective of developing a methodology for the establishment of neurological prognostic schemes obtained through peripheral markers in the blood stream and that can be applied to SUS and also a preclinical study in primates submitted to experimental stroke and treated with minocycline, in order to implement, in Brazil, a possible neuroprotective agent for human stroke that can be used, in the same way, in SUS. Project approved and financed by the FAPESPA Public Notice 003/2012 PPSUS, Research Program for SUS: Shared Health Management FAPESPA / MS-DECIT / CNPq / SESPA - PPSUS – 2011 Network / PA.
Status: Completed
Nature: Research
Students involved: Undergraduate Courses: (2) / Doctorate: (1)
Members: Rafael Rodrigues Lima - Member / Rosália Mendes Otero - Member / Wallace Gomes-Leal - Coordinator / Antonio Pereira Júnior - Member.
Funding: Paraense Amazon Foundation for Research Support - Financial Aid
2011 - 2013
Microglial Phenotypes, Neuroprotection and Endogenous Neurogenesis after Striatic Ischemia Induced by Cerebral Artery Experimental Occlusion
Description:
Funded by CNPq Universal Notice (2011). This project aims to investigate the patterns of activation of microglial cells presenting beneficial and harmful phenotypes and its relation with endogenous neurogenesis after focal striatal ischemia, evaluating that microglial populations are inhibited by tetracycline minocycline and by deletion of cells Mac1 + with saporin-conjugated antibodies after experimental middle cerebral artery occlusion (MCAO).
Status: Completed
Nature: Research
Members: Rafael Rodrigues Lima - Member / Wallace Gomes-Leal – Coordinator
2009 - 2012
Neurobehavioral Effects, Neuroprotectants, and Functional Recovery Following Blockade of Microglial Activation with Minocycline in Rats Undergoing Focal Ischemia in the Motor Cortex When Chronically Treated with Etoh from Adolescence to Adulthood
Description: Project funded by PPP Public Notice – FAPESPA 2009. The consumption of ethanol is one of the risk factors in the occurrence of cerebral ischemias and the therapy in the reversal or minimization of these effects is still obscure. This study aims to evaluate the effects of minocycline as a possible therapeutic option, through morphological, behavioral and antioxidant analyzes. Mice will undergo chronic treatment with ethanol (from puberty to adulthood), and then ischemiated in the motor area of the cerebral cortex. After ischemia, the animals will be treated with minocycline and subsequently submitted to behavioral motor tests and to histological and biochemical analyzes.
Status: Completed
Nature: Research
Members: Rafael Rodrigues Lima - Member / Cristiane do Socorro Ferraz Maia – Coordinator
Number of S, T & A Productions: 1
2009 - 2012
Consolidating Research with Cell Therapy in the State of Pará: translational studies for the development of stem cell therapies in the brain and spinal cord of rodents and Amazonian primates submitted to acute neural disorders
Description:
Funded by the PRONEX / FAPESPA / CNPq Public Notice – 2009. It aims to investigate the neuroprotection and functional recovery patterns of ischemic animals or with transplanted acute medullary lesions, through two routes of administration with bone marrow stem cells and, at the same time, submitted blockade of microglial activation by minocycline. To evaluate if the inflammatory block creates a more pleasant environment to the neuroprotective actions of transplanted stem cells.
Status: Completed
Nature: Research
Members: Rafael Rodrigues Lima - Member / Wallace Gomes-Leal – Coordinator
2008 - 2010
Endogenous Neurogenesis Induced by Experimental Vascular Brain Accident after Selective Blockade of Microglial / Macrophagic Activation with a Poly (ADP-Ribose) Polymerase Inhibitor (PJ34)
Description: To investigate whether selective blocking of hyperactivated microglial / macrophage aggregate formation is beneficial for experimental AVENC-induced striatal neurogenesis in the adult brain.
Status: Completed
Nature: Research
Students involved: Academic Master’s: (1) Doctorate: (5)
Members: Rafael Rodrigues Lima - Member / Wallace Gomes Leal - Coordinator / Edna Cristina Franco – Member
Funding: National Council for Scientific and Technological Development - Financial Aid
2008 - 2010
Blockade of Microglial Activation for the Maximization of Neuroprotective Effects of Bone Marrow Mesenchymal Stem Cells Transplanted Endovenously after Experimental Cerebrovascular accident
Description:
Funded by the Universal Public Notice – CNPq – 2008. One of the main hypotheses proposed for the beneficial effects of bone marrow stem cell transplantation is that this procedure would amplify endogenous repair mechanisms. On the other hand, it is known that microglial activation during experimental ischemia impairs neurogenesis in regions of the CNS, such as the hippocampus. In addition, recent studies suggest that the inflammatory environment, including microglial activation, influences the therapeutic properties of transplanted stem cells. An important issue is the right moment to perform the transplantation after the occurrence of stroke. The same should be done in the acute phase of the disease, when the inflammatory process is more intense, or in the more chronic phase, when the inflammatory response is more lenient. It is possible that the therapeutic actions of the transplanted stem cells are influenced by the inflammatory process. There are no studies that have investigated the influence of the inflammatory response on the therapeutic actions of the transplanted cells. The present project intends to investigate whether the blockade of the microglial response in transplanted animals with bone marrow stem cells induces more effective neuroprotection, neurogenesis and motor recovery, as evaluated by histological techniques and behavioral analysis. Based on the above, this project will investigate the hypothesis that the treatment of ischemic animals with bone marrow stem cells in concomitance with the blockade of microglial activation may induce tissue protection, neurogenesis and functional recovery more effective than simple therapy without blocking the inflammatory response. Our preliminary studies suggest that microglial blockade favors the therapeutic actions of bone marrow stem cells transplanted endovenously into the ischemic motor cortex, creating a less inflammatory environment that favors neuroprotection.
Status: Completed
Nature: Research
Members: Rafael Rodrigues Lima - Member / Cristovam Wanderley Picanço-Diniz - Member / Rosália Mendes Otero - Member / Carlos Alexandre Netto - Member / Edna Cristina Franco - Member / Wallace Gomes-Leal – Coordinator
Funding: National Council for Scientific and Technological Development - Financial Aid
2005 - 2007
Systematic Study of Neuropathological, Glial, Axonal and Inflammatory Neuropathological Changes in the Central Nervous System of Adult Mice Submitted to Cerebral Vascular Accident and Experimental Excitotoxic Injury
Description:
In this project, we investigated neuropathological patterns after induction of focal ischemia and excitotoxic injury in different areas of the white and gray matter of the central nervous system of adult rats. We are especially interested in the patterns of glial injury and activation, inflammatory reaction, myelin degeneration and axonal injury.
Status: Completed
Nature: Research
Students involved: Doctorate: (6)
Members: Rafael Rodrigues Lima - Member / Renata Duarte de Souza - Member / Walace Gomes-Leal - Coordinator / Cláudia Dourado dos Santos - Member / Vânia Castro Correa - Member / Enio Maurício - Member / Joanilson Guimarães Silva - Member.
Funding: Universidade da Amazônia - Financial Aid
2005 - 2007
Plasticity and Inflammatory Phenomena in the Treatment of Ischemic Brain Injury and Spinal Cord Injury with Stem Cells
Project certified by Coordinator Walace Gomes Leal on 03/22/2013.
Description:
In this project, we will investigate the possible neuroprotective and / or regenerative effects of bone marrow stem cells in adult rats submitted to experimental focal ischemia through the injection of endothelin-1. The project is carried out in collaboration with Dr. Rosália Mendes Otero of the Institute of Biophysics of UFRJ and with Dr. Carlos Alexandre Neto of UFRGS.
Status: Completed
Nature: Research.
Members: Rafael Rodrigues Lima - Member / Rosália Mendes Otero - Member / Carlos Alexandre Netto - Member / Wallace GOMES-LEAL - Coordinator.
Funding: National Council for Scientific and Technological Development - Financial Aid
2004 - 2006
Experimental Neuropathology of Amazonian Arboviruses
Description:
The present project has the general objective of establishing the model of viral infection by arbovirus of the family Rabdoviridae in adult mice and to study the neuropathological effects underlying this infection. As specific objectives, we intend to establish the temporal evolution of the lesion patterns induced by the experimental infection of adult mice with the following arboviruses: Itacaiúnas, Marabá, Curionópolis, Cocal, Carajás, Jurona and Piri, to map the distribution of arboviruses mentioned in the central nervous system of mice to describe histopathological changes underlying viral infection, with emphasis on necrotic, apoptotic, inflammatory and glial mechanisms as well as ultra-structural changes in the central nervous system of arboviruses infected animals.
Status: In progress
Nature: Research
Students involved: Undergraduate Courses: (8) / Academic Master’s: (1)
Members: Rafael Rodrigues Lima - Member / Walace Gomes-Leal - Coordinator.
Funding: Federal University of Pará – Cooperação
Number of S, T & A Productions: 1
2004 - 2006
Production of Didactic-Pedagogical Material for the Teaching of Morphology and Pathology in Dentistry: an interdisciplinary approach to the clinic
Description: Project funded by Federal University of Pará via PROINT 2004-2005. The project aims to create new teaching materials for the teaching of morphology and pathology in the Dentistry course. To prepare this material, Cebus apella monkeys will be used, which after induced periapical lesion, some animals will be sacrificed at different survival times to evaluate the lesion progression and inflammatory response. Others, after injury, will be treated endodontically, where new endodontic drugs recently launched in the market will be tested. In this project, the material obtained with the research will be later adopted as didactic material.
Status: In progress
Nature: Research
Members: Rafael Rodrigues Lima - Coordinator / Lucídia Fonseca Santiago - Member / Suely Maria Santos Lamarão - Member / Leila Marques Araújo - Member / Priscila Affonso - Member / Luciane Ribeiro Silva – Member
2003 - 2004
Production of Didactic Material for the Teaching of Morphology and Endodontics: an interdisciplinary look
Description:
Project approved by PROINT / 2003, which aims to implement an integration between the teaching of morphology and endodontics through the elaboration of several teaching resources, such as new histological slides and diaphanized teeth.
Status: In progress
Nature: Research.
Students involved: Undergraduate Courses: (2) / Specialization: (0) / Academic Master’s: (0) / Professional Master’s: (0) / Doctorate: (0)
Members: Rafael Rodrigues Lima - Member / Ana Paula Neves Gilho - Member / Lucídia Fonseca Santiago - Member / Suely Maria Santos Lamarão - Coordinator / Roberta Souza Dalmeida Couto – Member
Funding: Federal University of Pará - Financial Aid
2449380 - Roberta Souza D Almeida Couto (613.700.702-25)
2018 - Current
Prevalence of Non-Carious Cervical Lesions in Bodybuilders and Their Associated Risk Factors
Description:
LCNCs (Portuguese acronym) are frequent diseases, common in the clinical routine and have high incidence and prevalence rates independent of age, gender, and socioeconomic-cultural status of the patient, and the pathology is dependent on the lifestyle of the human being. The present study aims to estimate the prevalence of LCNC among bodybuilders and to relate their lifestyle as predisposing cofactors to the appearance of this dental pathology.
Status: In progress
Nature: Research
Students involved: Undergraduate Courses: (1) / Academic Master’s: (1)
Members: Roberta Souza D'Almeida Couto - Coordinator / Tamea Lacerda Monteiro Medeiros - Member / Ana Carolina Castilho Alves – Member
2017 - Current
Evaluation of Properties of Biodentine Compared to Other Biomaterials Used as Pulps
Description: The evolution of materials for pulp capping (CP – Portuguese acronym) has enabled treatments with higher success rates and less unwanted effects, being of great relevance to study the properties of the new Biomaterials available for CP. The objective of this study was to evaluate the dimensional stability, pH, calcium ion release, cell proliferation and biodentine (BD) mineralization in comparison with mineral trioxide aggregate (MTA) and calcium hydroxide cement (CHC).
Status: In progress
Nature: Research
Students involved: Undergraduate Courses: (1) / Academic Master’s: (1) / Doctorate: (1)
Members: Roberta Souza D'almeida Couto - Coordinator / Maria Stella Moreira - Member / Marques, Márcia Martins - Member / Thais de Mendonça Petta - Member / Daniela Feitosa Saavedra - Member / Ana Clara Pedroni - Member.
2010 - 2016
Effect of Substances Released by Pulp Capping Materials [Calcium Hydroxide, MTA and Copaiba Oil (Copaifera Sp)] on Proliferation, Differentiation, and Migration of Human Dental Pulp Stem Cells
Description:
Direct pulp capping materials are widely used in dental practice to maintain the vitality of the dental pulp. They should be primarily biocompatible and biostimulating, that is, capable of modulating the response of pulp tissue to aggression. These materials are mainly those based on calcium hydroxide [Ca (OH) 2] and, more recently, the mineral trioxide aggregate (MTA). However, other materials could also be used for direct pulp capping, among them the copaiba oil (Copaifera sp) due to its anti-inflammatory properties. The objective of this study was to analyze in vitro the proliferation, differentiation and migration of human dental pulp stem cells in response to the substances released by Ca (OH) 2 , MTA and copaiba oil (Copaifera sp).
Status: Completed
Nature: Research.
Students involved: Doctorate: (1)
Members: Roberta Souza D'Almeida Couto - Member / Roberta Souza D'Almeida Couto Santiago - Member / Márcia Martins Marques - Coordinator / Roberta Souza D`Almeida Couto – Member
Number of S, T & A Productions: 4
2009 - 2010
Anthropometric Profile and Level of Physical Activity of High School Students in the Municipality of Castanhal-Pará
Description:
To study habits and behaviors related to health, especially in the phase of childhood and adolescence, critical periods for the establishment of various habits of life and that tend to be maintained in adult life, becoming more difficult to be changed. Thus, the project aims to identify adolescents with health risk factors in the municipality of Castanhal-Pará by assessing levels of physical activity and anthropometric profile in schoolchildren, in order to contribute to the development of public policies and programs to promote activities physical education in a population dimension, mainly school, in order to encourage children and adolescents to adopt an active lifestyle, which is an action of great importance, since it may contribute not only to the acquisition of health benefits, but also to favor acquisition of habits and positive attitudes regarding the practice of physical activity that can be maintained in adult life.
Status: Desativado
Nature: Research
Students involved: Undergraduate Courses: (2)
Members: Roberta Souza D'Almeida Couto - Coordinator / Elton Vinícius Oliveira de Sousa - Member / Josafa G. Barreto – Member
Number of S, T & A Productions: 1
2007 - 2009
Clinical-Epidemiological Profile of Oral Health in Patients with Hansen's Disease
Description:
Leprosy (Hansen’s Disease) is a global disease, being distributed on all continents. In the Americas, Brazil emerges with the highest endemicity and ranks second in the world scenario, whose area of greatest commitment is the Legal Amazon. It is a disease of great importance to public health, results in physical disabilities and affects the skin, peripheral nerves and mucous membranes, especially the respiratory tract, affecting other organs such as the liver, testicles, eyes and oral cavity. The objective of this study was to evaluate the clinical and epidemiological profile of oral health in patients with leprosy in a polychemotherapeutic treatment (MDT) through clinical evaluation of oral mucosa, epidemiological indicators IPV (visible plaque index), ISG (gingival bleeding index), ICPOD (index of decayed, missing and filled teeth) and salivary flow measurement by the ISS (salivary secretion index), as well as the buffering capacity for the salivary pH.
Status: Completed
Nature: Research
Students involved: Academic Master’s: (1)
Members: Roberta Souza D'Almeida Couto - Member / Roberta Souza D'Almeida Couto Santiago - Coordinator / Regina Fátima Feio Barroso - Member / Cláudio Guedes Salgado - Member / Roberta Souza D`Almeida Couto – Member
Number of S, T & A Productions: 3
1640012 - Sandro Cordeiro Loretto (604.094.012-34)
2017 - Current
Does the Presence of the Glass Fiber Pin Influence the Clinical Longevity of Endodontically Treated and Restored Teeth with Composite Resin? Systematic review
Status: In progress
Nature: Research
Students involved: Undergraduate Courses: (1) / Academic Master’s: (2) / Doctorate: (1) .
Members: Sandro Cordeiro Loretto - Coordinator / Mara Eliane Soares Ribeiro - Member / Marcela Baraúna Magno - Member / Lucianne Cople Maia - Member / Juliana Costa Pereira Baía - Member / Pedro Lucas Bezerra Pinto – Member
2016 - 2016
Are the Presence and Intensity of Adult Dental Sensitivity Influenced by the Presence of Calcium in Bleaching Agents? A Systematic Review
Description:
A systematic review of randomized controlled clinical studies that show the presence and / or severity of dental bleaching sensitivity after treatment with peroxides containing calcium in its composition will be performed. To perform the review, the following question will be asked: are the presence and intensity of tooth sensitivity in adults affected by the incorporation of calcium in bleaching agents? There will be a review of the literature in the following electronic databases: Pubmed, Scopus, Web of science, Lilacs, Open Gray, Clinical trials, The Cochrane Library and EMBASE. No restrictions shall be imposed on the date of publication and the language used in the primary studies. All relevant studies will be translated and reviewed. The search strategy will be appropriately adapted to each database. The studies will be independently selected by two reviewers. Disagreements shall be resolved by consensus or, in the absence of consensus, by decision of a third reviewer. Each selected study will be read and its methodology will be evaluated in relation to the risk of bias. There will be a summary of the included study data, covering a description of the intervention protocol, characteristics of the study participants and the results.
Status: Completed
Nature: Research
Students involved: Undergraduate Courses: (2) / Academic Master’s: (1)
Members: Sandro Cordeiro Loretto - Coordinator / Patrícia Timbó Soares - Member / Verena Eiras da Silva - Member / Jéssica Lorrayne Gorayeb Pinheiro - Member
Number of counselings: 1
2015 - Current
O Substrato Bubalino é um Substituto Adequado ao Humano nos Testes de Resistência de União dos Materiais Odontológicos
Description: Since the adhesion of restorative materials to the tooth structure was achieved, where the first step was the enamel acid conditioning introduced by Buonocore (1955), this factor has represented a great achievement in the development of restorative dentistry, allowing for more conservative clinical treatments. Garcia (2007) describes that such technique makes possible the accomplishment of aesthetic, conservative and preventive restorative procedures. Since then, several generations of adhesive systems have been presented and all with the same purpose: to provide greater bond strength between the adhesive material and the dental remnant. Therefore, the need for evaluation and comparison of the adhesive capacity of the materials offered for clinical applicability is clear, given the wide range of these materials (Kadam et al., 2013; Grassi et al., 2012). According to Yanssen et al. (2013), the difficulty of obtaining sample size with quality and the impossibility of standardization (control of the source and age of the human teeth), the risks of infection and the ethical aspects involved, has made the teeth of mammalian animals used as alternatives to human teeth in in vitro and in situ studies. What had previously been recommended (Rueggeberg et al., 1991; Mellberg et al., 1992; Zero et al., 1995; Skene et al., 2002; Camargo et al., 2007). As substitutes, bovine teeth are widely used in the most diverse specialties, due to their micro and macroscopic similarity to human teeth, ease of acquisition, and low caries index. According to Schilke et al. (2000), the permanent inferior bovine incisors are frequently used due to the similarities of size and curvature with superior central incisors. The teeth of other mammalian animals (sheep, horses and pigs) have also been used as substitutes for human teeth in dental research. Nogueira et al., (2014) compared the ultrastructure and physical properties of bovine, bubaline and human enamel, concluding that the morphology, microhardness and mineral composition of the dental enamel of the animals analyzed were similar to human dental tissue. In this way, it made the bubaline substrate - enamel level - a reference for later studies. In this sense, the literature is still limited comparing the buffalo dental substrates to the human, and totally non-existent as to its use as another alternative for bond strength tests. Other clarifications must also be made regarding the morphological characteristics of bubaline dentin and to evaluate if there is similarity of this one with human dentin. According to Barbosa (2005) and Santana et al. (2011), it is mainly in the Northern region of Brazil where the buffalo herd can be found (about 50%). Thus, it is evident that, by offering the bubaline substrate as another viable and efficient alternative to the human substrate in mechanical tests, this will entail more subsidies for the accomplishment of several tests within the research in Dentistry. This research will focus on understanding the evolution of dental materials and can be performed to evaluate the bond strength of these dental substrates.
Status: In progress
Nature: Research
Students involved: Undergraduate Courses: (2) / Academic Master’s: (1)
Members: Sandro Cordeiro Loretto - Coordinator / Mário Honorato Silva e Souza Júnior - Member / Bárbara Catarina Lima Nogueira - Member / Rafael Rodrigues Lima - Member / Juliana Costa Pereira Baía - Member / Jackelyne Noriko Kikuchi de Freitas - Member.
Number of counselings: 2
2014 - Current
Effect of Excessive Dental Bleaching on Properties of Dental Substrates and Restorative Materials
Efeito do Clareamento Dental Excessivo nas Propriedades dos Substratos Dentais e Materiais Restauradores
Project certified by Coordinator Mário Honorato da Silva e Souza Junior on 07/20/2017.
Description:
The search for a harmonic smile, coupled with a lack of proper professional guidance, may expose patients to excessive bleaching treatments, which can be detrimental to the integrity of the dental elements. Thus, the present project aims to evaluate the effects of excessive exposure to different modalities of bleaching treatment on the morphology and mechanical properties of dental substrates and restorative materials.
Status: In progress
Nature: Research
Students involved: Undergraduate Courses: (4) / Academic Master’s: (6)
Members: Sandro Cordeiro Loretto - Member / Mário Honorato Silva e Souza Júnior - Coordinator / Mara Eliane Soares Ribeiro - Member / Bárbara Catarina Lima Nogueira - Member / Kelly Fernanda Barbosa Vilhena - Member / Rafael Rodrigues Lima - Member / Juliana Costa Pereira Baía - Member / Taíse Sousa Pamplona da Silva - Member / Patrícia Timbó Soares - Member / Roberta Pimentel de Oliveira - Member / Arianne Alexandre de Moraes - Member / Luciara Rocha de Melo - Member / Heitor Sales de Barros Santos – Member
Number of counselings: 5
2013 - 2015
Effect of Sodium Ascorbate on the Activity of Metalloproteinases in Bleached Dentin
Status: Completed
Nature: Research
Students involved: Undergraduate Courses: (1) / Academic Master’s: (1)
Members: Sandro Cordeiro Loretto - Coordinator / Mário Honorato Silva e Souza Júnior - Member / Mara Eliane Soares Ribeiro - Member / Gláucia Cristina Rodrigues Nascimento - Member / João de Jesus Viana Pinheiro - Member / Sérgio Melo Alves - Member.
Funding: Coordination of Improvement of Higher Level Personnel - Scholarship / Paraense Amazon Foundation for Research Support - Scholarship
2013 - 2014
Solutions for Dentinous Tube Disobliteration: methodological contribution to the in vitro studies of dentin hypersensitivity
Description:
Dentin hypersensitivity (HD – Portuguese acronym) is a clinical condition that causes dental discomfort in an increasing number of people. The incidence can range from 4% to 74% in the population and affects most individuals between the ages of 20 and 40 years (BARTOLD, 2006). Pain is episodic, short-lived and acute in nature (WEST, 2008). Two conditions must occur for the onset of HD: the dentin must be exposed and the dentinal tubule system open (PAROLIA, KUNDABALA, MOHAN, 2011). A number of investigations have determined characteristics of how the structure and surface of the sensitive dentin differs from the non-sensitive. One of these aspects is related to the greater permeability of the sensitive dentin (MARKOWITZ, PASHLEY, 2008). The literature describes a variety of chemical compounds with various concentrations and various forms of use to remove smear layer and to simulate a hypersensitive dentin in vitro. Some solutions such as EDTA and citric acid are known to be used for this purpose. However, despite extensive documentation of the effect of these agents on dentin tissue, there are few studies with similar methodologies, concentrations and comparable time intervals, which limits the ability to make valid inferences between these treatments (PEREZ-HEREDIA et al., 2008 (Roche et al., 2009). Recently, there has been a development of a technique through which it has been observed that it is possible to remove smear layer and open dentinal tubules by reasonably rapid exposure to an acid solution (pIHA 56), which is unsaturated compared to dentin (SHELLIS, CUTIS, 2010). The treatment of dentin with chelating solutions can be relatively aggressive, which may lead to significant demineralization, resulting in the formation of a layer of demineralized collagen matrix and removal of peritubular dentin (REIS et al., 2008; SHELLIS, CUTIS, 2010). Moreover, in the literature there is.
Status: Completed
Nature: Research
Students involved: Undergraduate Courses: (1) / Academic Master’s: (2)
Members: Sandro Cordeiro Loretto - Coordinator / Mário Honorato Silva e Souza Júnior - Member / Narjara Condurú Fernandes da Silva - Member / Aline Cristina Silva de Amorim - Member / Juliana Dias de Aguiar – Member
2012 - 2013
Intra-Chamber Temperature Variation of Restored Teeth with Compound Resins During the Office Bleaching Procedures
Description:
It is important to evaluate the temperature variation in the pulp chamber when photoactivated bleaching was performed on restorative teeth with methacrylate based composites with different inorganic filler content.
Status: Completed
Nature: Research
Students involved: Undergraduate Courses: (1) / Academic Master’s: (1)
Members: Sandro Cordeiro Loretto - Coordinator / Mário Honorato Silva e Souza Júnior - Member / Igor Studart Medeiros - Member / Esther Marina França Braga - Member / Mara Eliane Soares Ribeiro – Member
Number of counselings: 1
2012 - 2013
Influence of Desensitizing Dental Creams Based on Arginine, Strontium Acetate and Novamin on the Resistance of the Adhesive Union of Self-etching Systems to Dentin
Description: Conservative therapies, such as the application of desensitizing agents, should be preferentially indicated in order to reduce dentin hypersensitivity, in detriment of less conservative ones. However, in some situations, these techniques may be insufficient, requiring intervention through more invasive procedures, such as restorative procedures. Therefore, it is of fundamental importance to verify if the previous obliteration of the dentinal tubules obtained by these substances, would be able to interfere in the adhesion of restorative systems to the dentin surface.
Status: Completed
Nature: Research
Students involved: Undergraduate Courses: (1) / Academic Master’s: (1)
Members: Sandro Cordeiro Loretto - Coordinator / Mário Honorato Silva e Souza Júnior - Member / Aline Cristina Silva de Amorim - Member / Juliana Dias de Aguiar - Member / Igor Studart Medeiros - Member.
Funding: Federal University of Pará – Scholarship
Number of counselings: 1
2011 - 2014
Clinical Study of Restorations Made in Cervical, Non-Carious, Saturated Cavities with Ethanol Prior to Application of a Hydrophobic Adhesive Agent
Description: The aim of this clinical study will be to perform a clinical, longitudinal, prospective, randomized, blind and paired study of composite resin restorations in non-carious cervical cavities, performing dentin saturation with 100% ethanol prior to the application of a hydrophobic adhesive solvated in 10% of ethanol in relation to the following aspects: 1 - Postoperative sensitivity 2 - Marginal adaptation 3 - Retention.
Status: Completed
Nature: Research.
Students involved: Undergraduate Courses: (1) / Academic Master’s: (2)
Members: Sandro Cordeiro Loretto - Member / Mário Honorato Silva e Souza Júnior - Coordinator / Patrícia de Almeida Rodrigues Silva e Souza - Member / Joyce Figueira de Araújo - Member / Railson de Oliveira Ferreira - Member / Thaís Andrade de Figueiredo – Member
Funding: Coordination of Improvement of Higher Level Personnel - Financial Aid / Paraense Amazon Foundation for Research Support – Scholarship
2011 - 2012
Influence of Professional Desensitizers Based on Calcium and Arginine Phosphate on Dentin Adhesion Resistance
Description:
Conservative therapies, such as the application of desensitizing agents, should be preferentially indicated in order to reduce dentin hypersensitivity, in detriment of less conservative ones. However, in some situations, these techniques may be insufficient, requiring intervention through more invasive procedures, such as restorative procedures. Therefore, it is of fundamental importance to verify if the previous obliteration of the dentinal tubules obtained by these substances, would be able to interfere in the adhesion of restorative systems to the dentin surface.
Status: Completed
Nature: Research
Students involved: Undergraduate Courses: (1) / Academic Master’s: (1)
Members: Sandro Cordeiro Loretto - Coordinator / Tamea Lacerda Monteiro - Member / Narjara Condurú Fernandes da Silva – Member
2011 - 2012
Evaluation of Microhardness and Scanning Electron Microscopy of the Dentinary Surface after Brushing with Different Desensitizing Dental Creams and Erosive Challenge with Amazonian Fruit
Description:
Considering the prevalence of HD in the adult population and the specific diet of the Amazonian population, rich in acid fruit consumption such as cupuaçu and taperebá, the new bases for the control of HD (arginine, strontium acetate and biosilicate / Novamin) available under the form of toothpaste, the importance of desensitizing treatments to withstand the acidic challenges of the daily diet; it becomes relevant to evaluate the efficacy of the desensitizing dentifrices based on arginine, strontium acetate and biosilicate, Colgate Pro-Relief, Sensodyne Rapid Relief and Repair and Protect, respectively, on the dentin surface and dentinal tubular sealing after acid challenge with fruits Amazon, cupuaçu and taperebá.
Status: Completed
Nature: Research.
Students involved: Doctorate: (1)
Members: Sandro Cordeiro Loretto - Coordinator / Max Pinto da Costa da Rocha - Member / Ana Cecília Corrêa Aranha – Member
2009 - 2012
Influence of Dental Bleaching on Roughness Composite Low Contraction Surface Resin
Description: To evaluate the influence of different tooth bleaching techniques on the surface roughness of the new composite resin formulations known as low polymerization contraction.
Status: Completed
Nature: Research
Students involved: Undergraduate Courses: (2)
Members: Sandro Cordeiro Loretto - Coordinator / Mário Honorato Silva e Souza Júnior - Member / Cecy Martins Silva - Member / Breno Suano de Farias - Member / Nayara Cristina Monteiro Carneiro – Member
Funding: Coordination of Improvement of Higher Level Personnel - Financial Aid / Paraense Amazon Foundation for Research Support – Scholarship
2009 - Current
Evaluation of the Flexural Strength of Glass Fiber Pines and Their Relation to Chemical Composition and Quantity of Fibers Determined by SEM, EDS and X-Ray Diffraction
Description:
Increasingly fiber-reinforced polymer pins are employed in dentistry. There are numerous brands and types, and the greater interest in this type of pin is related to its characteristics of resilience and aesthetics. However, the literature has shown differences in clinical behavior and, in some situations, it is perceived a relatively high incidence of fractures of these pins. Thus, this work has as main objective to perform a characterization study of different brands of pins with glass fiber reinforcement, aiming to evaluate fiber density and composition by means of scanning electron microscopy (SEM), scattered energy spectroscopy (EDS) and X-ray diffraction, tests that will be carried out with the collaboration of the Institute of Geosciences of the UPFA. Mechanical tests will also be performed to determine the flexural strength of these pins and attempt to correlate these values of mechanical strength with the characteristics of the reinforcing fibers. This will require equipment to perform the flexural strength test. In this specific study (Evaluation of the flexural strength of glass vibrating pins and its relation with the chemical composition and quantity of fibers determined by SEM, EDS and X-ray diffraction), the requested equipment will be employed to determine the flexural resistance of pins of glass fiber reinforced polymers. This type of test is necessary because within the buccal cavity the teeth reconstructed with this anchoring feature are submitted to different types and directions of forces, in this case, the transverse forces, which lead to the folding and flexing of the pins, the most and, over time, lead to the failure of restorative procedures. It is important to stress that a large number of tests can be performed with this type of equipment, such as compression, traction, shear, torsion, etc. This study mentioned serves only as an example of i.
Status: In progress
Nature: Research
Students involved: Academic Master’s: (2)
Members: Sandro Cordeiro Loretto - Member / Elisa Burlamaqui Klautau - Member / Karina Gama Kato Carneiro - Member / Mário Honorato Silva e Souza Júnior - Coordinator / Cecy Martins Silva - Member / Marcelo Figueiredo Lobato - Member / Bruno Pereira Alves – Member
Funding: Coordination of Improvement of Higher Level Personnel - Financial Aid
1640241 - Sergio de Melo Alves Junior (561.467.012-20)
2015 - Current
Evaluation of Tissue, Biochemistry and Proteomics of Salivary Glands in Animal Models of Metal Exposure
Description:
This project aims to evaluate the effects of systemic exposure to potentially toxic metals such as mercury, aluminum, lead and cadmium, common in mining or mineral exploration areas in the Amazon. The mining activity, legal and clandestine, together with the waste dumped in the rivers from the large mining companies make the Amazon region a region where toxiciological damages from exposure to metals can be considered endemic, being one of the greatest challenges in public health. The objective is to simulate the toxicological damages caused by environmental exposure to rodent salivary glands. For this purpose, a variety of toxicological evaluation parameters will be used, from the measurement of metal deposits in the glandular parenchyma, analysis of oxidative biochemistry, proteomic evaluation and tissue / immunohistochemical characterization of the reaction and / or tissue components damage in salivary glands.
Status: In progress
Nature: Research
Members: Sérgio de Melo Alves Junior - Member / Rafael Rodrigues Lima - Coordinator / De Jesus Viana Pinheiro, João – Member
2014 - Current
Immunohistochemical Expression of the Notch-1, ADAM-12, HIF-1 and HB-EGF Proteins in Benign and Malignant Neoplasms of the Head and Neck Region
Description: It is known that in neoplastic proliferation of solid tumors there is an intratumoral hypoxia environment, from which the HIF-1, NOTCH-1, ADAM-12 and HB-EGF proteins can be activated which, through their signaling pathway, have a prominent role in invasiveness and in the process of tumor metastasis. Thus, the objective of this project is the immunoexpression of the HIF-1, NOTCH-1, ADAM-12 and HB-EGF proteins in cases of benign and malignant neoplasms of the head and neck region. The immunoperoxidase technique will be employed using the anti-HIF-1, anti-NOTCH1, anti-ADAM-12 and anti-HB-EGF antibodies. The immunoexpression will be evaluated by the percentage of marked area of images acquired in the AxioScope microscope equipped with AxioCamHRc camera, with a 40x objective.
Status: In progress
Nature: Research
Members: Sérgio de Melo Alves Junior - Member / João de Jesus Pinheiro - Coordinator / Fabrício Mesquita Tuji – Member
2013 - Current
Isolation and Characterization of Cells Derived from Epidermoid Carcinoma and Mucoepidermoid Carcinoma. In Vitro Study of the Relation between Metallothionein Expression, Matrix Metalloproteinases, Growth Factors and Cell Proliferation Events
Description:
Recently, studies have reported that the expression of some metallothionein (MT) isoforms correlates with cell proliferation in some malignancies, especially the MT2A isoform (THIRUMOORTHY et al., 2011; KIM et al., 2010). MMP-1 expression has been correlated with the expression of MMPs in some studies, which reported a direct correlation of the overexpression of MT2A and increased expression of MMP-9 (KIM et al., 2010; RIBEIRO et al., 2011). In addition, researchers have pointed out that growth factors play an important role in signaling for MT expression (LLORENS et al., 2011). In addition, the project proposes the characterization of cell line derived from mucoepidermoid carcinoma and human squamous cell carcinoma by transcriptomic analysis. This analysis is one of the main tools to isolate and characterize genes, as well as to study the interactions between them. In this context, the mechanisms involved in the expression and interaction of genes, as well as the understanding of functional networks established by proteins, make the transcriptomic analysis in evidence in the current scientific scenario.
Status: In progress
Nature: Research
Students involved: Undergraduate Courses: (1) / Academic Master’s: (2)
Members: Sérgio de Melo Alves Junior - Coordinator / Fabrício Mesquita Tuji - Member / João de Jesus Viana Pinheiro - Member / EDUARDO CRUZ - Member / Dimitra Castelo Branco - Member / Aline Marques Dias - Member.
Funding: National Council for Scientific and Technological Development - Financial Aid
2012 - 2016
In Vitro Study of the Relation between Metallothionein Expression, Matrix Metalloproteinases, Growth Factors and Events of Cell Proliferation in Cystic Adenoid Carcinoma
Description:
Cystic adenoid carcinoma (CAC) is a malignant neoplasm, especially affecting the small salivary glands, accounting for about 10% to 15% of all tumors in the head and neck region (NEVILLE 2009, KHAN AJ et al., 2001, NAMAZIE et al., 2001). This neoplasm often gives rise to slow, painless but highly invasive lesions with high recurrence rates, distant metastasis and mortality (KHAFIF et al., 2005). Histologically, it is characterized by showing epithelial and myoepithelial cells arranged in histological patterns called cribiform, tubular and solid (PEREZ et al., 2006). Many studies suggest that each of the histological subtypes of CAC is related to different clinical behaviors (SZANTO et al., 1984, ALVES et al., 2007, WG I et al., 2005). Tumor invasion inherent to CAC is a complex process mediated, among other factors, by cell proliferation and localized proteolysis of the extracellular matrix, mainly performed by enzymes called matrix metalloproteinases (MMPs). Among these proteases, MMP-2 and -9 have a prominent role in the invasiveness of tumors, since they degrade several components of the matrix, such as collagen (STERNLICHT, WERB, 2001; ZHANG et al., 2009). It is well known that MMPs are zinc dependent endopeptidases to exert their enzymatic function. (Birkedal-Hansen et al., 2003). Thus, the phenomena related to MMPs can occur with the help of metallothionein, a protein that functions as an intracellular reservoir of zinc ions for the activation of these proteases, as well as certain transcription factors. A study of the expression of metallothionein in ameloblastoma suggests not only its pro-mitotic and antiapoptotic role, but also its function as a reservoir of zinc ions for important proteases (RIBEIRO et al., 2011, Cing, 2011). In addition, Alves et al. (2007) showed that, when the cell is at rest, metallothionein is located in the cytoplasm.
Status: Completed
Nature: Research
Students involved: Undergraduate Courses: (2) / Academic Master’s: (2) / Doctorate: (1)
Members: Sérgio de Melo Alves Junior - Coordinator / João de Jesus Pinheiro - Member / Maria Sueli da Silva Kataoka - Member / José Antônio Picanço Diniz Junior - Member / Adriane Sousa de Siqueira - Member / Aline Semblano Dias Carreira - Member / Marina Rolo Pinheiro da Rosa - Member / Jaeger, Ruy G. - Member / Lara Pinto - Member / João Aquime – Member
Funding: National Council for Scientific and Technological Development - Financial Aid
2012 - Current
Application of Amazonian Herbal Medicine in Oncology
Description: One of the most frequent oral complications of chemotherapy is mucositis, a change in inflammatory character for which there is no definite treatment yet. This oral complication causes severe discomfort and pain, makes feeding difficult, and may result in discontinuation of the antineoplastic treatment. Considering the clinical relevance of mucositis, it is important to find ways to treat it. Carapa Guianensis Aubl, popularly known as “Andiroba” has active principles that gives this phytotherapeutic potential anti-inflammatory, analgesic and antiallergenic. This set of characteristics aroused the attention to study the action of Andiroba in the treatment of oral mucositis (OM) symptomatology in patients on cancer treatment. This Research Line aims to evaluate the potential action potential of Andiroba (Carapa Guianensis Aubl) on two fronts. In the healing of oral mucositis induced by 5-fluorouracil (5-FU) in hamsters, through clinical, histopathological and immunohistochemical evaluation and in cancer patients submitted to chemotherapy.
Status: In progress
Nature: Research
Members: Sérgio de Melo Alves Junior - Member / Fabrício Mesquita Tuji - Coordinator / João de Jesus Viana PINHEIRO – Member
2010 - 2012
In Vitro Study of the Interrelation between Expression of Growth Factors and Expression of Matrix Metalloproteinases in Pleomorphic Adenoma
Estudo In Vitro da Inter-Relação entre a Expressão de Fatores de Crescimento e a Expressão de Metaloproteinases da Matriz no Adenoma Pleomórfico
Description: Among the neoplasias that affect the salivary glands, the most frequent is the pleomorphic adenoma. Despite its slow growth and benign behavior, there is evidence that the neoplastic and stromal cells of this tumor secrete MMPs, possibly indicating the participation of these enzymes in their process of local invasiveness. However, the expression of MMPs and their regulatory mechanisms in pleomorphic adenoma have been poorly documented. The development of tumor cell lines is a procedure that has been used by several researchers in order to better understand the biological behavior of the most varied neoplasms in vitro, as well as to contribute to the advances in the prognosis of the lesion, diagnosis and therapeutics. The previous establishment and characterization of a cell line derived from human pleomorphic adenoma will allow us to carry out more mechanistic studies using gene silencing (siRNA). The methods that will be used will allow correlating the expression of growth factors with the expression of MMPs. According to the established correlation, it is expected to elucidate the way in which growth factors can modulate the expression of MMPs and their participation in local remodeling and invasiveness mechanisms of this neoplasm. Proof of the correlation of these molecules may be of great importance for the treatment of pleomorphic adenoma.
Status: In progress
Nature: Research
Members: Sérgio de Melo Alves Junior - Member / João de Jesus Pinheiro - Coordinator / Maria Sueli da Silva Kataoka - Member / José Antônio Picanço Diniz Junior - Member / Ruy Gastaldoni Jaeger - Member / Adriane Sousa de Siqueira - Member / Aline Semblano Dias Carreira - Member / Nayara Picanço Kataoka - Member / Nelson Antonio Bailão Ribeiro - Member / Maria Clara Rodrigues Pinheiro – Member
Funding: National Council for Scientific and Technological Development - Financial Aid
2010 - 2012
Isolation and Characterization of Ameloblastoma-Derived Cells. In Vitro Study of Modulation of Matrix Metalloproteinases by Growth Factors
Description:
This project will enable the implementation of new technologies in the Dentistry undergraduate and graduate courses at the Federal University of Pará, resulting, therefore, in the fulfillment of what is recommended in the recent initiatives of the research development agencies of the country, regarding the greater integration of educational institutions, in terms of Research and graduate studies, see the need to consolidate Research in emerging institutions and consequently their Graduate Programs, which depends on the qualification of their professors and technicians. The development of tumor cell lines is a procedure that has been used by several researchers in order to better understand the biological behavior of the most varied neoplasms in vitro, as well as to contribute to the advances in the prognosis of the lesion, diagnosis and therapeutics. The methods used will allow the characterization of recent isolation of tumor parenchyma and stroma cells, which is believed to be a primary cell line originated from an Ameloblastoma. This method, therefore, will allow the execution of more mechanistic studies where, using genetic silencing, we hope to correlate the growth factors with the synthesis of MMPs. According to the established correlation, an important step has been taken in elucidating the way in which growth factors can modulate the release of MMPs, their possible interactions with TIMPs and the participation of these growth factors with remodeling and invasion site of this neoplasm. On the other hand, several factors can alter normal cell regulation affecting the function of the genes that control cell division and repair, apoptosis, and angiogenesis. Mutations in DNA may include large rearrangements such as translocations, inversions, amplifications and deletions of genes, as well as nucleotide mutations with insertions and deletions of bases.
Status: In progress
Nature: Research
Students involved: Undergraduate Courses: (3) / Academic Master’s: (1)
Members: Sérgio de Melo Alves Junior - Coordinator / João de Jesus Pinheiro - Member / Ruy Gastaldoni Jaeger - Member / Maria Sueli da Silva Kataoka - Member / José Antônio Picanço Diniz Junior - Member / Aline Semblano Dias CARREIRA - Member / Nayara Picanço Kataoka - Member / Nelson Antonio Bailão Ribeiro - Member / Fabrício Mesquita Tuji - Member / Alann Thaffarell Portilho de Souza - Member / Marina Rolo Pinheiro da Rosa – Member
Funding: National Council for Scientific and Technological Development - Financial Aid
2009 - 2011
Oral Health in the Island of Marajó: diagnosis of the problems and proposal of the implementation of public policies of dentistry
Description:
The Marajó archipelago, given the size of the social demands, due to the structural lack of basic services and its environmental characteristics, presents high levels of poverty, resulting in low levels of education, high rates of infant mortality, morbidity due to endemic diseases and, consequently, scarce health services, resulting in one of the lowest levels of quality of life in the country. Oral diseases are part of this overwhelming picture, culminating in a process of social exclusion and low human development. The epidemiology of oral diseases in Brazil in recent years shows a more encouraging picture of the knowledge of the diseases and the possibility of planning new services. This breath is not the same in all regions of the country and the Northern region still needs knowledge of the epidemiology of diseases and better access to services, since the surveys of oral health conditions in the State of Pará are scarce, making it difficult to plan actions that cover the treatment needs of the population. Most of the epidemiological data available refer to the metropolitan area of Belém. This project proposes to collect epidemiological data and to know the profile of oral health in municipalities located in Marajó Island, in the State of Pará. The sample will consist of 1,171 individuals, distributed according to the size of each selected municipality, observing the age groups or ages indexes of 5 years, 12 years, 15 to 19 years, 35 to 44 years and 64 to 74 years. The oral health conditions related to dental caries and periodontal disease, the most prevalent diseases of the oral cavity, will be raised; use and need of dental prosthesis, aiming at the adequate functionality of the stomatognathic system; and oral neoplasms, which is one of the primary neoplastic sites most incident to proportional cancer mortality. All withdrawals will be made through the indices.
Status: In progress
Nature: Research
Students involved: Undergraduate Courses: (2) / Academic Master’s: (2)
Members: Sérgio de Melo Alves Junior - Member / João de Jesus Pinheiro - Member / Maria das Graças Rodrigues Pinheiro - Member / Maria Sueli da Silva Kataoka - Member / Regina Fátima Feio Barroso - Coordinator / Marizeli Viana de Aragão Araújo - Member / Alda França Costa - Member / Ceci Baker de Melo - Member.
Funding: Paraense Amazon Foundation for Research Support - Financial Aid
2008 - 2011
Epidemiological Survey of Oral Health in the Municipalities of Curuçá, Maracanã, Salinópolis and São João de Pirabas Salgado Region of the State of Pará
Description:
This project is a consortium between the Dentistry Graduate Programs of both UFPA and UFMG, aiming at the interaction of epidemiological surveys of caries and oral cancer, the use of phytotherapeutic drugs for popular use and its anti-neoplastic action in Cell Cuture, bringing to this study an epidemiological and biotechnological profile.
Status: In progress
Nature: Research.
Students involved: Undergraduate Courses: (2) / Academic Master’s: (1)
Members: Sérgio de Melo Alves Junior - Member / João de Jesus Pinheiro - Coordinator / Maria das Graças Rodrigues Pinheiro - Member / Maria Sueli da Silva Kataoka - Member / José Antônio Picanço Diniz Junior - Member / Ricardo Alves de Mesquita - Member / Regina Fátima Feio Barroso - Member / Erick Nelo Pedreira - Member / Maria Cássia Ferreira Aguiar - Member / Marizeli Viana de Aragão Araújo – Member
Funding: Paraense Amazon Foundation for Research Support – Financial Aid
2004 - 2007
Analysis of the Expression and Mechanisms of Action of the COX-2 Protein in Cultured Cells of Human Oral Epidermoid Carcinoma
Status: Completed
Nature: Research
Students involved: Doctorate: (1)
Members: Sérgio de Melo Alves Junior - Coordinator / Décio dos Santos Pinto Junior – Member